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Expression of ICOS in the salivary glands of patients with primary Sjogren's syndrome and its molecular mechanism

  • Authors:
    • Ping Li
    • Yi Jin
    • Rui Zhao
    • Zhonghui Xue
    • Juan Ji
  • View Affiliations / Copyright

    Affiliations: Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China, Department of Rheumatology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 348
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    Published online on: September 28, 2022
       https://doi.org/10.3892/mmr.2022.12864
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Abstract

The present study aimed to explore latent markers for identifying primary Sjogren's syndrome (pSS), as well as their expression and molecular mechanism. Hub inducible T cell co‑stimulator genes were retrieved from the Gene Expression Omnibus. A total of 95 patients with pSS and 68 healthy individuals from Affiliated Hospital of Nantong University (Nantong, China) were included in the study. The expression of inducible T cell co‑stimulator (ICOS) in whole blood and saliva was evaluated using ELISA. Western blotting was performed to investigate aquaporin 5 (AQP5) protein expression, as well as the inflammatory effects of ICOS in patients with pSS compared with healthy individuals. Differentially expressed mRNAs were analyzed in whole blood (GSE84844) and salivary gland (GSE40611) of patients with pSS. In total, 15 hub genes were identified, among which ICOS was indicated to serve a role in the most pivotal immunity pathways. pSS was markedly associated with inflammatory pathways. Results from the present study found that ICOS was upregulated in the salivary gland, whole blood and saliva of patients with pSS. Salivary weight was negatively correlated with the levels of ICOS in the saliva of patients with pSS. The expression of AQP5 was markedly lower in patients with pSS. The expression of AQP5 was negatively associated with ICOS. Compared with that of healthy individuals, the expression of ICOS and inflammatory factors was higher and the expression of AQP5 was lower in pSS patients as assessed by western blotting. These data demonstrated that ICOS may affect AQP5 expression by promoting inflammation in the salivary glands of patients with pSS.
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Copy and paste a formatted citation
Spandidos Publications style
Li P, Jin Y, Zhao R, Xue Z and Ji J: Expression of ICOS in the salivary glands of patients with primary Sjogren's syndrome and its molecular mechanism. Mol Med Rep 26: 348, 2022.
APA
Li, P., Jin, Y., Zhao, R., Xue, Z., & Ji, J. (2022). Expression of ICOS in the salivary glands of patients with primary Sjogren's syndrome and its molecular mechanism. Molecular Medicine Reports, 26, 348. https://doi.org/10.3892/mmr.2022.12864
MLA
Li, P., Jin, Y., Zhao, R., Xue, Z., Ji, J."Expression of ICOS in the salivary glands of patients with primary Sjogren's syndrome and its molecular mechanism". Molecular Medicine Reports 26.5 (2022): 348.
Chicago
Li, P., Jin, Y., Zhao, R., Xue, Z., Ji, J."Expression of ICOS in the salivary glands of patients with primary Sjogren's syndrome and its molecular mechanism". Molecular Medicine Reports 26, no. 5 (2022): 348. https://doi.org/10.3892/mmr.2022.12864
Copy and paste a formatted citation
x
Spandidos Publications style
Li P, Jin Y, Zhao R, Xue Z and Ji J: Expression of ICOS in the salivary glands of patients with primary Sjogren's syndrome and its molecular mechanism. Mol Med Rep 26: 348, 2022.
APA
Li, P., Jin, Y., Zhao, R., Xue, Z., & Ji, J. (2022). Expression of ICOS in the salivary glands of patients with primary Sjogren's syndrome and its molecular mechanism. Molecular Medicine Reports, 26, 348. https://doi.org/10.3892/mmr.2022.12864
MLA
Li, P., Jin, Y., Zhao, R., Xue, Z., Ji, J."Expression of ICOS in the salivary glands of patients with primary Sjogren's syndrome and its molecular mechanism". Molecular Medicine Reports 26.5 (2022): 348.
Chicago
Li, P., Jin, Y., Zhao, R., Xue, Z., Ji, J."Expression of ICOS in the salivary glands of patients with primary Sjogren's syndrome and its molecular mechanism". Molecular Medicine Reports 26, no. 5 (2022): 348. https://doi.org/10.3892/mmr.2022.12864
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