Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
December-2022 Volume 26 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2022 Volume 26 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Calcium‑dependent activation of PHEX, MEPE and DMP1 in osteocytes

  • Authors:
    • Baris Ozgur Donmez
    • Ege Riza Karagur
    • Aysegul Cort Donmez
    • Jaeyoung Choi
    • Ozan Akkus
  • View Affiliations / Copyright

    Affiliations: Department of Anatomy, School of Medicine, Pamukkale University, Denizli 20160, Turkey, Department of Medical Genetics, School of Medicine, Pamukkale University, Denizli 20160, Turkey, Department of Medical Biochemistry, School of Medicine, Pamukkale University, Denizli 20160, Turkey, School of Dental Medicine, Case Western Reserve University, Cleveland, OH 44106, USA, Department of Mechanical and Aerospace Engineering, Case Western Reserve University, Cleveland, OH 10900, USA
    Copyright: © Donmez et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 359
    |
    Published online on: October 20, 2022
       https://doi.org/10.3892/mmr.2022.12876
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Calcium (Ca2+) signaling is the first messenger signal exhibited by osteocytes. The present study aimed to better understand the link between Ca2+ concentration, and the levels of bone mineralization regulator proteins [phosphate‑regulating neutral endopeptidase on chromosome X (PHEX), matrix extracellular phosphoglycoprotein (MEPE) and dentin matrix protein 1 (DMP1)] and the levels of oxidative stress in osteocytes. The viability of MLO‑Y4 cells was determined using the live/dead assay following treatment with various Ca2+ concentrations (1.8, 6, 12, 18, 24 and 50 mM) for different durations (15 and 60 min, and 24 h). Superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and NADPH oxidase (NOX) enzymes were analyzed using a colorimetric method. Apoptosis was detected by caspase‑3 analysis. Furthermore, the protein expression levels of PHEX, MEPE and DMP1 were analyzed using immunoblotting, and oxidative stress was examined using the total antioxidant and total oxidant status (TOS) assay. Notably, after 15 min, there were more live cells than dead cells; however, after 60 min, the number of dead cells was increased following treatment with 24 and 50 mM Ca2+. After 24 h, there were more dead cells than live cells following treatment with 50 mM Ca2+. After 24 h of Ca2+ treatment, the highest protein expression levels of PHEX, MEPE and DMP1 were measured in cells treated with 24 mM Ca2+. In addition, as Ca2+ concentration increased, the TOS and the oxidative stress index values were also increased. In conclusion, these results suggested that 24 mM Ca2+ may trigger bone mineralization proteins, such as PHEX, MEPE and DMP1, and could be considered an applicable dosage for the treatment of bone damage in the future.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

View References

1 

Palumbo C and Ferretti M: The osteocyte: From ‘Prisoner’ to ‘Orchestrator’. J Funct Morphol Kinesiol. 6:282021. View Article : Google Scholar : PubMed/NCBI

2 

Florencio-Silva R, da Silva Sasso GR, Sasso-Cerri E, Simões J and Cerri PS: Biology of bone tissue: Structure, function, and factors that influence bone cells. Biomed Res Int. 2015:4217462015. View Article : Google Scholar : PubMed/NCBI

3 

Kato Y, Windle JJ, Koop BA, Mundy GR and Bonewald LF: Establishment of an osteocyte-like cell line, MLO-Y4. J Bone Miner Res. 12:2014–2023. 1997. View Article : Google Scholar : PubMed/NCBI

4 

Robling AG and LF, . Bonewald the osteocyte: New insights. Annu Rev Physiol. 82:485–506. 2020. View Article : Google Scholar : PubMed/NCBI

5 

Kao RS, Abbott MJ, Louie A, O'Carroll D, Lu W and Nissenson R: Constitutive protein kinase A activity in osteocytes and late osteoblasts produces an anabolic effect on bone. Bone. 55:277–287. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Fisher LW and Fedarko NS: Six genes expressed in bones and teeth encode the current members of the SIBLING family of proteins. Connect Tissue Res. 44 (Suppl 1):S33–S40. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Guo D, Keightley A, Guthrie J, Veno PA, Harris SE and Bonewald LF: Identification of osteocyte-selective proteins. Proteomics. 10:3688–3698. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Lu Y, Yuan B, Qin C, Cao Z, Xie Y, Dallas SL, McKee MD, Drezner MK, Bonewald LF and Feng JQ: The biological function of DMP-1 in osteocyte maturation is mediated by its 57-kDa C-terminal fragment. J Bone Miner Res. 26:331–340. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Siyam A, Wang S, Qin C, Mues G, Stevens R, D'Souza RN and Lu Y: Nuclear localization of DMP1 proteins suggests a role in intracellular signaling. Biochem Biophys Res Commun. 424:641–646. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Ling Y, Rios HF, Myers ER, Lu Y, Feng JQ and Boskey AL: DMP1 depletion decreases bone mineralization in vivo: An FTIR imaging analysis. J Bone Miner Res. 20:2169–2177. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Rowe PS, de Zoysa PA, Dong R, Wang HR, White KE, Econs MJ and Oudet CL: MEPE, a new gene expressed in bone marrow and tumors causing osteomalacia. Genomics. 67:54–68. 2000. View Article : Google Scholar : PubMed/NCBI

12 

David V, Martin A, Hedge AM and Rowe PSN: Matrix extracellular phosphoglycoprotein (MEPE) is a new bone renal hormone and vascularization modulator. Endocrinology. 150:4012–4023. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Lu C, Huang S, Miclau T, Helms JA and Colnot C: Mepe is expressed during skeletal development and regeneration. Histochem Cell Biol. 121:493–499. 2004. View Article : Google Scholar : PubMed/NCBI

14 

Jung H, Mbimba T, Unal M and Akkus O: Repetitive short-span application of extracellular calcium is osteopromotive to osteoprogenitor cells. J Tissue Eng Regen Med. 12:e1349–e1359. 2018. View Article : Google Scholar : PubMed/NCBI

15 

Jung H, Best M and Akkus O: Microdamage induced calcium efflux from bone matrix activates intracellular calcium signaling in osteoblasts via L-type and T-type voltage-gated calcium channels. Bone. 76:88–96. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Yamauchi M, Yamaguchi T, Kaji H, Sugimoto T and Chihara K: Involvement of calcium-sensing receptor in osteoblastic differentiation of mouse MC3T3-E1 cells. Am J Physiol Endocrinol Metab. 288:E608–E616. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Boskey AL and Roy R: Cell culture systems for studies of bone and tooth mineralization. Chem Rev. 108:4716–4733. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Bonewald LF, Harris SE, Rosser J, Dallas MR, Dallas SL, Camacho NP, Boyan B and Boskey A: Von Kossa staining alone is not sufficient to confirm that mineralization in vitro represents bone formation. Calcif Tissue Int. 72:537–547. 2003. View Article : Google Scholar : PubMed/NCBI

19 

Zhou H, Wu T, Dong X, Wang Q and Shen J: Adsorption mechanism of BMP-7 on hydroxyapatite (001) surfaces. Biochem Biophys Res Commun. 361:91–96. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Magne D, Bluteau G, Lopez-Cazaux S, Weiss P, Pilet P, Ritchie HH, Daculsi G and Guicheux J: Development of an odontoblast in vitro model to study dentin mineralization. Connect Tissue Res. 45:101–108. 2004. View Article : Google Scholar : PubMed/NCBI

21 

Price PA, June HH, Hamlin NJ and Williamson MK: Evidence for a serum factor that initiates the re-calcification of demineralized bone. J Biol Chem. 279:19169–19180. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Erel O: A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 38:1103–1111. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Sheweita SA and Khoshhal KI: Calcium metabolism and oxidative stress in bone fractures: Role of antioxidants. Curr Drug Metab. 8:519–525. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Shuai C, Liu G, Yang Y, Qi F, Peng S, Yang W, He C, Wang G and Qian G: A strawberry-like Ag-decorated barium titanate enhances piezoelectric and antibacterial activities of polymer scaffold. Nano Energy. 74:1048252000. View Article : Google Scholar

25 

Shuaia C, Xu Y, Feng P, Wang G, Xiong S and Peng S: Antibacterial polymer scaffold based on mesoporous bioactive glass loaded with in situ grown silver. Chemical Engineering J. 374:304–345. 2019. View Article : Google Scholar

26 

Maeno S, Niki Y, Matsumoto H, Morioka H, Yatabe T, Funayama A, Toyama Y, Taguchi T and Tanaka J: The effect of calcium ion concentration on osteoblast viability, proliferation and differentiation in monolayer and 3D culture. Biomaterials. 26:4847–4855. 2005. View Article : Google Scholar : PubMed/NCBI

27 

Welldon KJ, Findlay DM, Evdokiou A, Ormsby RT and Atkins GJ: Calcium induces pro-anabolic effects on human primary osteoblasts associated with acquisition of mature osteocyte markers. Mol Cell Endocrinol. 376:85–92. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Sugimoto T, Kanatani M, Kano J, Kaji H, Tsukamoto T, Yamaguchi T, Fukase M and Chihara K: Effects of high calcium concentration on the functions and interactions of osteoblastic cells and monocytes and on the formation of osteoclast-like cells. J Bone Miner Res. 8:1445–1452. 1993. View Article : Google Scholar : PubMed/NCBI

29 

Dvorak MM, Siddiqua A, Ward DT, Carter DH, Dallas SL, Nemeth EF and Riccardi D: Physiological changes in extracellular calcium concentration directly control osteoblast function in the absence of calciotropic hormones. Proc Natl Acad Sci USA. 101:5140–5145. 2004. View Article : Google Scholar : PubMed/NCBI

30 

Mullen CA, Haugh MG, Schaffler MB, Majeska RG and McNamara LM: Osteocyte differentiation is regulated by extracellular matrix stiffness and intercellular separation. J Mech Behav Biomed Mater. 28:183–194. 2013. View Article : Google Scholar : PubMed/NCBI

31 

Fulzele K, Lai F, Dedic C, Saini V, Uda Y, Shi C, Tuck P, Aronson JL, Liu X, Spatz JM, et al: Osteocyte-Secreted Wnt Signaling Inhibitor Sclerostin Contributes to Beige Adipogenesis in Peripheral Fat Depots. J Bone Miner Res. 32:373–384. 2017. View Article : Google Scholar : PubMed/NCBI

32 

Uda Y, Azab E, Sun N, Shi C and Pajevic PD: Osteocyte mechanobiology. Curr Osteoporos Rep. 15:318–325. 2017. View Article : Google Scholar : PubMed/NCBI

33 

Sarban S, Kocyigit A, Yazar M and Isikan UE: Plasma total antioxidant capacity, lipid peroxidation, and erythrocyte antioxidant enzyme activities in patients with rheumatoid arthritis and osteoarthritis. Clin Biochem. 38:981–986. 2005. View Article : Google Scholar : PubMed/NCBI

34 

Schroder K: NADPH oxidases in bone homeostasis and osteoporosis. Free Radic Biol Med. 132:67–72. 2019. View Article : Google Scholar : PubMed/NCBI

35 

Ermak G and Davies KJ: Calcium and oxidative stress: From cell signaling to cell death. Mol Immunol. 38:713–721. 2002. View Article : Google Scholar : PubMed/NCBI

36 

Ostman B, Michaëlsson K, Helmersson J, Byberg L, Gedeborg R, Melhus H and Basu S: Oxidative stress and bone mineral density in elderly men: Antioxidant activity of alpha-tocopherol. Free Radic Biol Med. 47:668–673. 2009. View Article : Google Scholar : PubMed/NCBI

37 

Banfi G, Iorio EL and Corsi MM: Oxidative stress, free radicals and bone remodeling. Clin Chem Lab Med. 46:1550–1555. 2008. View Article : Google Scholar : PubMed/NCBI

38 

Domazetovic V, Marcucci G, Iantomasi T, Brandi ML and Vincenzini MT: Oxidative stress in bone remodeling: Role of antioxidants. Clin Cases Miner Bone Metab. 14:209–216. 2017. View Article : Google Scholar : PubMed/NCBI

39 

Jilka RL, Noble B and Weinstein RS: Osteocyte apoptosis. Bone. 54:264–271. 2013. View Article : Google Scholar : PubMed/NCBI

40 

Zhong ZM, Bai L and Chen JT: Advanced oxidation protein products inhibit proliferation and differentiation of rat osteoblast-like cells via NF-kappaB pathway. Cell Physiol Biochem. 24:105–114. 2009. View Article : Google Scholar : PubMed/NCBI

41 

Nakashima T, Hayashi M, Fukunaga T, Kurata K, Oh-Hora M, Feng JQ, Bonewald LF, Kodama T, Wutz A, Wagner EF, et al: Evidence for osteocyte regulation of bone homeostasis through RANKL expression. Nat Med. 17:1231–1234. 2011. View Article : Google Scholar : PubMed/NCBI

42 

Yu C, Huang D, Wang K, Lin B, Liu Y, Liu S, Wu W and Zhang H: Advanced oxidation protein products induce apoptosis, and upregulate sclerostin and RANKL expression, in osteocytic MLO-Y4 cells via JNK/p38 MAPK activation. Mol Med Rep. 15:543–550. 2017. View Article : Google Scholar : PubMed/NCBI

43 

Wang Y, Branicky R, Noë A and Hekimi S: Superoxide dismutases: Dual roles in controlling ROS damage and regulating ROS signaling. J Cell Biol. 217:1915–1928. 2018. View Article : Google Scholar : PubMed/NCBI

44 

Kobayashi K, Nojiri H, Saita Y, Morikawa D, Ozawa Y, Watanabe K, Koike M, Asou Y, Shirasawa T, Yokote K, et al: Mitochondrial superoxide in osteocytes perturbs canalicular networks in the setting of age-related osteoporosis. Sci Rep. 5:91482015. View Article : Google Scholar : PubMed/NCBI

45 

Lushchak VI: Glutathione homeostasis and functions: Potential targets for medical interventions. J Amino Acids. 2012:7368372012. View Article : Google Scholar : PubMed/NCBI

46 

Nandi A, Yan LY, Jana CK and Das N: Role of catalase in oxidative stress- and age-associated degenerative diseases. Oxid Med Cell Longev. 2019:96130902019. View Article : Google Scholar : PubMed/NCBI

47 

Miyagawa K, Yamazaki M, Kawai M, Nishino J, Koshimizu T, Ohata Y, Tachikawa K, Mikuni-Takagaki Y, Kogo M, Ozono K and Michigami T: Dysregulated gene expression in the primary osteoblasts and osteocytes isolated from hypophosphatemic Hyp mice. PLoS One. 9:e938402014. View Article : Google Scholar : PubMed/NCBI

48 

Wang X, Wang S, Li C, Gao T, Liu Y, Rangiani A, Sun Y, Hao J, George A, Lu Y, et al: Inactivation of a novel FGF23 regulator, FAM20C, leads to hypophosphatemic rickets in mice. PLoS Genet. 8:e10027082012. View Article : Google Scholar : PubMed/NCBI

49 

Bellido T: Osteocyte-driven bone remodeling. Calcif Tissue Int. 94:25–34. 2014. View Article : Google Scholar : PubMed/NCBI

50 

Martin A, Liu S, David V, Li H, Karydis A, Feng JQ and Quarles LD: Bone proteins PHEX and DMP1 regulate fibroblastic growth factor Fgf23 expression in osteocytes through a common pathway involving FGF receptor (FGFR) signaling. FASEB J. 25:2551–2562. 2011. View Article : Google Scholar : PubMed/NCBI

51 

Lavi-Moshayoff V, Wasserman G, Meir T, Silver J and Naveh-Many T: PTH increases FGF23 gene expression and mediates the high-FGF23 levels of experimental kidney failure: A bone parathyroid feedback loop. Am J Physiol Renal Physiol. 299:F882–F889. 2010. View Article : Google Scholar : PubMed/NCBI

52 

Gluhak-Heinrich J, Pavlin D, Yang W, MacDougall M and Harris SE: MEPE expression in osteocytes during orthodontic tooth movement. Arch Oral Biol. 52:684–690. 2007. View Article : Google Scholar : PubMed/NCBI

53 

Yang W, Lu Y, Kalajzic I, Guo D, Harris MA, Gluhak-Heinrich J, Kotha S, Bonewald LF, Feng JQ, Rowe DW, et al: Dentin matrix protein 1 gene cis-regulation: use in osteocytes to characterize local responses to mechanical loading in vitro and in vivo. J Biol Chem. 280:20680–20690. 2005. View Article : Google Scholar : PubMed/NCBI

54 

Gluhak-Heinrich J, Ye L, Bonewald LF, Feng JQ, MacDougall M, Harris SE and Pavlin D: Mechanical loading stimulates dentin matrix protein 1 (DMP1) expression in osteocytes in vivo. J Bone Miner Res. 18:807–817. 2003. View Article : Google Scholar : PubMed/NCBI

55 

Bonewald LF: The role of the osteocyte in bone and nonbone disease. Endocrinol Metab Clin North Am. 46:1–18. 2017. View Article : Google Scholar : PubMed/NCBI

56 

Kulkarni RN, Bakker AD, Everts V and Klein-Nulend J: Inhibition of osteoclastogenesis by mechanically loaded osteocytes: involvement of MEPE. Calcif Tissue Int. 87:461–468. 2010. View Article : Google Scholar : PubMed/NCBI

57 

Xiong J, Onal M, Jilka RL, Weinstein RS, Manolagas SC and O'Brien CA: Matrix-embedded cells control osteoclast formation. Nat Med. 17:1235–1241. 2011. View Article : Google Scholar : PubMed/NCBI

58 

Zhang K, Barragan-Adjemian C, Ye L, Kotha S, Dallas M, Lu Y, Zhao S, Harris M, Harris SE, Feng JQ and Bonewald LF: E11/gp38 selective expression in osteocytes: Regulation by mechanical strain and role in dendrite elongation. Mol Cell Biol. 26:4539–4552. 2006. View Article : Google Scholar : PubMed/NCBI

59 

Zakhary I, Alotibi F, Lewis J, ElSalanty M, Wenger K, Sharawy M and Messer RLW: Inherent physical characteristics and gene expression differences between alveolar and basal bones. Oral Surg Oral Med Oral Pathol Oral Radiol. 122:35–42. 2016. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Donmez BO, Karagur ER, Donmez AC, Choi J and Akkus O: Calcium‑dependent activation of PHEX, MEPE and DMP1 in osteocytes. Mol Med Rep 26: 359, 2022.
APA
Donmez, B.O., Karagur, E.R., Donmez, A.C., Choi, J., & Akkus, O. (2022). Calcium‑dependent activation of PHEX, MEPE and DMP1 in osteocytes. Molecular Medicine Reports, 26, 359. https://doi.org/10.3892/mmr.2022.12876
MLA
Donmez, B. O., Karagur, E. R., Donmez, A. C., Choi, J., Akkus, O."Calcium‑dependent activation of PHEX, MEPE and DMP1 in osteocytes". Molecular Medicine Reports 26.6 (2022): 359.
Chicago
Donmez, B. O., Karagur, E. R., Donmez, A. C., Choi, J., Akkus, O."Calcium‑dependent activation of PHEX, MEPE and DMP1 in osteocytes". Molecular Medicine Reports 26, no. 6 (2022): 359. https://doi.org/10.3892/mmr.2022.12876
Copy and paste a formatted citation
x
Spandidos Publications style
Donmez BO, Karagur ER, Donmez AC, Choi J and Akkus O: Calcium‑dependent activation of PHEX, MEPE and DMP1 in osteocytes. Mol Med Rep 26: 359, 2022.
APA
Donmez, B.O., Karagur, E.R., Donmez, A.C., Choi, J., & Akkus, O. (2022). Calcium‑dependent activation of PHEX, MEPE and DMP1 in osteocytes. Molecular Medicine Reports, 26, 359. https://doi.org/10.3892/mmr.2022.12876
MLA
Donmez, B. O., Karagur, E. R., Donmez, A. C., Choi, J., Akkus, O."Calcium‑dependent activation of PHEX, MEPE and DMP1 in osteocytes". Molecular Medicine Reports 26.6 (2022): 359.
Chicago
Donmez, B. O., Karagur, E. R., Donmez, A. C., Choi, J., Akkus, O."Calcium‑dependent activation of PHEX, MEPE and DMP1 in osteocytes". Molecular Medicine Reports 26, no. 6 (2022): 359. https://doi.org/10.3892/mmr.2022.12876
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team