Coptisine attenuates post‑infectious IBS via Nrf2‑dependent inhibition of the NLPR3 inflammasome

  • Authors:
    • Ying Xiong
    • Hong Wei
    • Chong Chen
    • Lu Jiao
    • Juan Zhang
    • Yonggang Tan
    • Li Zeng
  • View Affiliations

  • Published online on: October 21, 2022     https://doi.org/10.3892/mmr.2022.12879
  • Article Number: 362
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Abstract

Inhibition of the activation of the NLR family pyrin domain‑containing 3 (NLRP3) inflammasome has previously been reported to confer protection against post‑infectious irritable bowel syndrome (PI‑IBS). Coptisine, the second most abundant isoquinoline alkaloid in Coptis chinensis, can inhibit NLRP3 inflammasome activation; however, whether coptisine exhibits protective effects against PI‑IBS remains unclear. In the present study, coptisine significantly reduced gastrointestinal motility and abdominal withdrawal reflex scores in a PI‑IBS rat model that was induced using intragastric administration of Trichinella spiralis larvae. Coptisine treatment significantly decreased the protein levels of oxidative stress markers, 4‑hydroxynonenal, protein carbonyl and 8‑hydroxy‑2'deoxyguanosine, and proinflammatory cytokines, TNF‑α, IL‑1β and IL‑18 in the colon of PI‑IBS rats. Moreover, coptisine treatment significantly increased nuclear factor erythroid 2‑related factor 2 (Nrf2) nuclear translocation and heme oxygenase‑1 protein expression levels, while significantly downregulating the protein expression levels of NLRP3, apoptosis‑associated speck‑like protein containing a CARD and caspase‑1 in the colons of PI‑IBS rats. It is important to note that the anti‑inflammatory effects of coptisine were blocked by the Nrf2 inhibitor ML385. In summary, the present study indicated that coptisine potentially attenuated PI‑IBS in rats via Nrf2‑dependent inhibition of the NLPR3 inflammasome.
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December-2022
Volume 26 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Xiong Y, Wei H, Chen C, Jiao L, Zhang J, Tan Y and Zeng L: Coptisine attenuates post‑infectious IBS via Nrf2‑dependent inhibition of the NLPR3 inflammasome. Mol Med Rep 26: 362, 2022
APA
Xiong, Y., Wei, H., Chen, C., Jiao, L., Zhang, J., Tan, Y., & Zeng, L. (2022). Coptisine attenuates post‑infectious IBS via Nrf2‑dependent inhibition of the NLPR3 inflammasome. Molecular Medicine Reports, 26, 362. https://doi.org/10.3892/mmr.2022.12879
MLA
Xiong, Y., Wei, H., Chen, C., Jiao, L., Zhang, J., Tan, Y., Zeng, L."Coptisine attenuates post‑infectious IBS via Nrf2‑dependent inhibition of the NLPR3 inflammasome". Molecular Medicine Reports 26.6 (2022): 362.
Chicago
Xiong, Y., Wei, H., Chen, C., Jiao, L., Zhang, J., Tan, Y., Zeng, L."Coptisine attenuates post‑infectious IBS via Nrf2‑dependent inhibition of the NLPR3 inflammasome". Molecular Medicine Reports 26, no. 6 (2022): 362. https://doi.org/10.3892/mmr.2022.12879