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Polysaccharides from Annona muricata leaves protect against cisplatin‑induced cytotoxicity in macrophages by alleviating mitochondrial dysfunction

  • Authors:
    • Jeong Moo Han
    • Ha-Yeon Song
    • Kwang-Il Kim
    • Woo Yong Park
    • Sang-Hyun Park
    • Eui-Baek Byun
    • Eui-Hong Byun
  • View Affiliations / Copyright

    Affiliations: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeollabuk‑do 56212, Republic of Korea, Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea, Department of Food Science and Technology, Kongju National University, Yesan‑eup, Chungcheongnam‑do 340‑800, Republic of Korea
    Copyright: © Han et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 16
    |
    Published online on: November 30, 2022
       https://doi.org/10.3892/mmr.2022.12903
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Abstract

Cisplatin is a prominent chemotherapeutic agent that can induce significant damage to normal cells. Therefore, it is important to develop agents that protect normal cells without influencing the chemotherapeutic effect of cisplatin. The present study was conducted to explore the protective effects of Annona muricata leaf polysaccharides (ALPS) against cisplatin‑induced toxicity in macrophages. Apoptosis was assessed in macrophages and lung cancer cells to investigate the cytoprotective effect of ALPS, their effect on the production of cisplatin‑induced reactive oxygen species (ROS) and the loss of the mitochondrial transmembrane potential (MTP). Cisplatin, when used alone or in combination with ALPS, showed significant toxicity against A549 and H460 lung cancer cells. However, cisplatin‑induced cytotoxicity was suppressed by cotreatment of RAW 264.7 macrophages with ALPS. ALPS significantly inhibited the upregulation of Bax, cytosolic cytochrome c and caspases‑3, ‑8 and ‑9. Moreover, ALPS resulted in the cleavage of PARP and downregulation of Bcl‑2 levels in a concentration‑dependent manner, which ultimately led to a reduction in the apoptotic and necrotic populations of cisplatin‑treated RAW 264.7 macrophages. The suppression of the apoptotic signaling pathways was mediated through the reduction of ROS and MTP loss in cisplatin‑treated RAW 264.7 macrophages. In addition, ALPS alleviated cell damage by suppressing the mitochondrial apoptotic pathways in cisplatin‑treated bone marrow‑derived macrophages. Together, these findings suggested that ALPS may alleviate the toxic side effects of chemotherapeutic agents and act as a potential candidate for use as an effective adjuvant therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Han JM, Song H, Kim K, Park WY, Park S, Byun E and Byun E: Polysaccharides from <em>Annona muricata</em> leaves protect against cisplatin‑induced cytotoxicity in macrophages by alleviating mitochondrial dysfunction. Mol Med Rep 27: 16, 2023.
APA
Han, J.M., Song, H., Kim, K., Park, W.Y., Park, S., Byun, E., & Byun, E. (2023). Polysaccharides from <em>Annona muricata</em> leaves protect against cisplatin‑induced cytotoxicity in macrophages by alleviating mitochondrial dysfunction. Molecular Medicine Reports, 27, 16. https://doi.org/10.3892/mmr.2022.12903
MLA
Han, J. M., Song, H., Kim, K., Park, W. Y., Park, S., Byun, E., Byun, E."Polysaccharides from <em>Annona muricata</em> leaves protect against cisplatin‑induced cytotoxicity in macrophages by alleviating mitochondrial dysfunction". Molecular Medicine Reports 27.1 (2023): 16.
Chicago
Han, J. M., Song, H., Kim, K., Park, W. Y., Park, S., Byun, E., Byun, E."Polysaccharides from <em>Annona muricata</em> leaves protect against cisplatin‑induced cytotoxicity in macrophages by alleviating mitochondrial dysfunction". Molecular Medicine Reports 27, no. 1 (2023): 16. https://doi.org/10.3892/mmr.2022.12903
Copy and paste a formatted citation
x
Spandidos Publications style
Han JM, Song H, Kim K, Park WY, Park S, Byun E and Byun E: Polysaccharides from <em>Annona muricata</em> leaves protect against cisplatin‑induced cytotoxicity in macrophages by alleviating mitochondrial dysfunction. Mol Med Rep 27: 16, 2023.
APA
Han, J.M., Song, H., Kim, K., Park, W.Y., Park, S., Byun, E., & Byun, E. (2023). Polysaccharides from <em>Annona muricata</em> leaves protect against cisplatin‑induced cytotoxicity in macrophages by alleviating mitochondrial dysfunction. Molecular Medicine Reports, 27, 16. https://doi.org/10.3892/mmr.2022.12903
MLA
Han, J. M., Song, H., Kim, K., Park, W. Y., Park, S., Byun, E., Byun, E."Polysaccharides from <em>Annona muricata</em> leaves protect against cisplatin‑induced cytotoxicity in macrophages by alleviating mitochondrial dysfunction". Molecular Medicine Reports 27.1 (2023): 16.
Chicago
Han, J. M., Song, H., Kim, K., Park, W. Y., Park, S., Byun, E., Byun, E."Polysaccharides from <em>Annona muricata</em> leaves protect against cisplatin‑induced cytotoxicity in macrophages by alleviating mitochondrial dysfunction". Molecular Medicine Reports 27, no. 1 (2023): 16. https://doi.org/10.3892/mmr.2022.12903
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