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Pueraria protein extract inhibits melanogenesis and promotes melanoma cell apoptosis through the regulation of MITF and mitochondrial‑related pathways

  • Authors:
    • Yuchu Zhao
    • Shiting Yu
    • Yue Wang
    • Yanyan Chen
    • Jingjing Chen
    • Jiawen Wang
    • Meichen Liu
    • Siming Wang
  • View Affiliations / Copyright

    Affiliations: Jilin Provincial Key Laboratory of Biomacromolecules of Chinese Medicine, Northeast Asian Research Institute of Traditional Chinese Medicine, Changchun University of Chinese Medicine, Changchun, Jilin 130117, P.R. China, College of Pharmacy, Changchun University of Chinese Medicine, Changchun, Jilin 130117, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 64
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    Published online on: February 2, 2023
       https://doi.org/10.3892/mmr.2023.12951
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Abstract

Pueraria Lobata Radix (P. Lobata Radix) is an edible traditional Chinese medicine that contains various active compounds. Proteins from P. Lobata Radix have become the subject of increased interest in recent years. In evaluating the whitening effect on the skin, the present study found that the P. Lobata Radix water‑soluble total protein extract (PLP) had the strongest inhibitory effect on tyrosinase activity. In the present study, the anti‑melanogenic effect of PLP and the inhibitory effect on B16 melanoma cells were investigated. PLP significantly reduced the tyrosinase activity and melanin content in B16 melanoma cells. Mechanistically, PLP inhibited melanogenesis by decreasing the expression of tyrosinase, tyrosinase‑related protein (TRP)‑1 and TRP‑2 through downregulation of the microphthalmia‑associated transcription factor (MITF) gene, which was mediated by inhibition of p38 mitogen‑activated protein kinase signaling. In addition, PLP inhibited cell viability and triggered apoptosis of B16 cells in a dose‑dependent manner. Exposure to PLP reduced the mitochondrial membrane potential (MMP) and decreased ATP generation, leading to mitochondria‑related apoptosis of B16 melanoma cells. The expression levels of succinate dehydrogenase (SDH) and its two related subunits (SDHA and SDHB) were downregulated significantly by PLP, which may be associated with the regulation of mitochondrial energy metabolism by PLP. These results may explain why MMP collapse and reduced ATP generation were observed in B16 melanoma cells treated with PLP. Finally, the present study demonstrated that the inhibition of melanin synthesis by PLP was correlated with the regulation of antioxidant enzymes to reduce reactive oxygen species levels. These results suggested that PLP inhibits melanogenesis by downregulating the expression of MITF‑related melanogenic enzymes and triggering apoptosis through mitochondria‑related pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao Y, Yu S, Wang Y, Chen Y, Chen J, Wang J, Liu M and Wang S: Pueraria protein extract inhibits melanogenesis and promotes melanoma cell apoptosis through the regulation of MITF and mitochondrial‑related pathways. Mol Med Rep 27: 64, 2023.
APA
Zhao, Y., Yu, S., Wang, Y., Chen, Y., Chen, J., Wang, J. ... Wang, S. (2023). Pueraria protein extract inhibits melanogenesis and promotes melanoma cell apoptosis through the regulation of MITF and mitochondrial‑related pathways. Molecular Medicine Reports, 27, 64. https://doi.org/10.3892/mmr.2023.12951
MLA
Zhao, Y., Yu, S., Wang, Y., Chen, Y., Chen, J., Wang, J., Liu, M., Wang, S."Pueraria protein extract inhibits melanogenesis and promotes melanoma cell apoptosis through the regulation of MITF and mitochondrial‑related pathways". Molecular Medicine Reports 27.3 (2023): 64.
Chicago
Zhao, Y., Yu, S., Wang, Y., Chen, Y., Chen, J., Wang, J., Liu, M., Wang, S."Pueraria protein extract inhibits melanogenesis and promotes melanoma cell apoptosis through the regulation of MITF and mitochondrial‑related pathways". Molecular Medicine Reports 27, no. 3 (2023): 64. https://doi.org/10.3892/mmr.2023.12951
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao Y, Yu S, Wang Y, Chen Y, Chen J, Wang J, Liu M and Wang S: Pueraria protein extract inhibits melanogenesis and promotes melanoma cell apoptosis through the regulation of MITF and mitochondrial‑related pathways. Mol Med Rep 27: 64, 2023.
APA
Zhao, Y., Yu, S., Wang, Y., Chen, Y., Chen, J., Wang, J. ... Wang, S. (2023). Pueraria protein extract inhibits melanogenesis and promotes melanoma cell apoptosis through the regulation of MITF and mitochondrial‑related pathways. Molecular Medicine Reports, 27, 64. https://doi.org/10.3892/mmr.2023.12951
MLA
Zhao, Y., Yu, S., Wang, Y., Chen, Y., Chen, J., Wang, J., Liu, M., Wang, S."Pueraria protein extract inhibits melanogenesis and promotes melanoma cell apoptosis through the regulation of MITF and mitochondrial‑related pathways". Molecular Medicine Reports 27.3 (2023): 64.
Chicago
Zhao, Y., Yu, S., Wang, Y., Chen, Y., Chen, J., Wang, J., Liu, M., Wang, S."Pueraria protein extract inhibits melanogenesis and promotes melanoma cell apoptosis through the regulation of MITF and mitochondrial‑related pathways". Molecular Medicine Reports 27, no. 3 (2023): 64. https://doi.org/10.3892/mmr.2023.12951
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