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Mechanism of dioscin ameliorating renal fibrosis through NF-κB signaling pathway-mediated inflammatory response

  • Authors:
    • Yang Wang
    • Peng Liu
    • Guijie Ma
    • Chenguang Wu
    • Wenhui Zhu
    • Pengce Sun
    • Wenya Lu
    • Xinyu Yang
    • Yilun Zhang
    • Na Liu
    • Ping Li
  • View Affiliations / Copyright

    Affiliations: Renal Division, Department of Medicine, Heilongjiang Academy of Chinese Medicine Sciences, Harbin, Heilongjiang 150036, P.R. China, Shunyi Hospital, Beijing Hospital of Traditional Chinese Medicine, Shunyi, Beijing 101300, P.R. China, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150036, P.R. China, Beijing Key Lab for Immune‑Mediated Inflammatory Diseases, China‑Japan Friendship Hospital, Beijing 100029, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 93
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    Published online on: March 22, 2023
       https://doi.org/10.3892/mmr.2023.12980
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Abstract

Dioscin (DIS) is a natural compound derived from Chinese herbal medicine. In recent years, multiple studies have reported that DIS has immunoregulation, anti‑fibrosis, anti‑inflammation, anti‑viral and anti‑tumor effects. However, the mechanism by which DIS ameliorates renal fibrosis and inflammation remains to be elucidated. The aim of the present study was to investigate the role of DIS in renal fibrosis and inflammation and to explore its underlying mechanism. It used network pharmacology to predict the targets of DIS for the treatment of renal interstitial fibrosis. The present study was performed using unilateral ureteral obstruction mice and HK‑2 cells in vivo and in vitro. The mice were treated with different doses of DIS. Kidney tissues were collected for histopathology staining, western blotting, immunohistochemistry staining and reverse transcription‑quantitative (RT‑q) PCR. TGF‑β1 (2 ng/ml) was used to induce renal fibrosis in the cells. Then, cells were respectively treated with DIS (3.125, 6.25, 12.5 µM) and Bay11‑7082 (an inhibitor of NF‑κB p65 nuclear transcription, 1 µM) for another 24 h. The expressions of inflammatory factors and NF‑κB pathway proteins were detected by immunofluorescence, ELISA, western blotting and RT‑qPCR. DIS alleviated renal injury in the UUO mice. Mechanistically, DIS not only decreased the expressions of inflammatory factors including IL‑1β, NOD‑like receptor thermal protein domain associated protein 3, monocyte chemotactic protein 1, IL‑6, TNF‑α and IL‑18 but also reduced the level of phosphorylation of NF‑κB p65 in vivo and in vitro, which was similar to the impact of Bay11‑7082. DIS ameliorated renal fibrosis by inhibiting the NF‑κB signaling pathway‑mediated inflammatory response, which may be a therapeutic pathway for delaying chronic kidney disease.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Liu P, Ma G, Wu C, Zhu W, Sun P, Lu W, Yang X, Zhang Y, Liu N, Liu N, et al: Mechanism of dioscin ameliorating renal fibrosis through NF-κB signaling pathway-mediated inflammatory response. Mol Med Rep 27: 93, 2023.
APA
Wang, Y., Liu, P., Ma, G., Wu, C., Zhu, W., Sun, P. ... Li, P. (2023). Mechanism of dioscin ameliorating renal fibrosis through NF-κB signaling pathway-mediated inflammatory response. Molecular Medicine Reports, 27, 93. https://doi.org/10.3892/mmr.2023.12980
MLA
Wang, Y., Liu, P., Ma, G., Wu, C., Zhu, W., Sun, P., Lu, W., Yang, X., Zhang, Y., Liu, N., Li, P."Mechanism of dioscin ameliorating renal fibrosis through NF-κB signaling pathway-mediated inflammatory response". Molecular Medicine Reports 27.4 (2023): 93.
Chicago
Wang, Y., Liu, P., Ma, G., Wu, C., Zhu, W., Sun, P., Lu, W., Yang, X., Zhang, Y., Liu, N., Li, P."Mechanism of dioscin ameliorating renal fibrosis through NF-κB signaling pathway-mediated inflammatory response". Molecular Medicine Reports 27, no. 4 (2023): 93. https://doi.org/10.3892/mmr.2023.12980
Copy and paste a formatted citation
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Spandidos Publications style
Wang Y, Liu P, Ma G, Wu C, Zhu W, Sun P, Lu W, Yang X, Zhang Y, Liu N, Liu N, et al: Mechanism of dioscin ameliorating renal fibrosis through NF-κB signaling pathway-mediated inflammatory response. Mol Med Rep 27: 93, 2023.
APA
Wang, Y., Liu, P., Ma, G., Wu, C., Zhu, W., Sun, P. ... Li, P. (2023). Mechanism of dioscin ameliorating renal fibrosis through NF-κB signaling pathway-mediated inflammatory response. Molecular Medicine Reports, 27, 93. https://doi.org/10.3892/mmr.2023.12980
MLA
Wang, Y., Liu, P., Ma, G., Wu, C., Zhu, W., Sun, P., Lu, W., Yang, X., Zhang, Y., Liu, N., Li, P."Mechanism of dioscin ameliorating renal fibrosis through NF-κB signaling pathway-mediated inflammatory response". Molecular Medicine Reports 27.4 (2023): 93.
Chicago
Wang, Y., Liu, P., Ma, G., Wu, C., Zhu, W., Sun, P., Lu, W., Yang, X., Zhang, Y., Liu, N., Li, P."Mechanism of dioscin ameliorating renal fibrosis through NF-κB signaling pathway-mediated inflammatory response". Molecular Medicine Reports 27, no. 4 (2023): 93. https://doi.org/10.3892/mmr.2023.12980
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