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Article Open Access

Thioredoxin1 is a target to attenuate diabetes‑induced RPE cell dysfunction in human ARPE19 cells by alleviating oxidative stress

  • Authors:
    • Hui Qi
    • Tianhe Liu
    • Jiasu Liu
    • Qiufeng Teng
    • Zhongping Ma
    • Shengnan Wang
    • Shihui Wen
    • Chenghong Zhang
    • Xiang Ren
    • Hui Kong
    • Li Kong

  • View Affiliations / Copyright

    Affiliations: Department of Histology and Embryology, College of Basic Medicine, Dalian Medical University, Dalian, Liaoning 116044, P.R. China, Department of Otorhinolaryngology, The Second Hospital, Dalian Medical University, Dalian, Liaoning 116023, P.R. China
    Copyright: © Qi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 134
    |
    Published online on: May 23, 2023
       https://doi.org/10.3892/mmr.2023.13021
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Abstract

Diabetes‑induced cell dysfunction of the retinal pigment epithelium (RPE) contributes to the initiation and progression of diabetic retinopathy (DR). Thioredoxin 1 (Trx1) plays a key role in DR. However, the effect and mechanism of Trx1 on diabetes‑induced cell dysfunction of the RPE is not fully understood during DR. In the present study, the effect of Trx1 on this process and its related mechanism were investigated. A Trx1 overexpression cell line, ARPE19‑Trx1/LacZ, was constructed and treated with or without high glucose (HG). Flow cytometry was used to analyze apoptosis of these cells and the mitochondrial membrane potential was analyzed using JC‑1 staining solution. A DCFH‑DA probe was also used to detect the reactive oxygen species (ROS) generation. Western blotting was used to examine the expression of related proteins in ARPE‑19 cells after HG treatment. The results demonstrated that the RPE layer was damaged in clinical samples. ROS formation and RPE cell dysfunction increased after HG treatment in vitro. Besides, the expression of mitochondrial‑mediated apoptosis related proteins (Bax, apoptosis‑inducing factor, cytochrome C, Caspase3 and Caspase9) also increased; however, overexpression of Trx1 attenuated these changes and improved the function of ARPE19 cells. These results indicated that overexpression of Trx1 alleviated diabetes‑induced RPE cell dysfunction in DR by attenuating oxidative stress.
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Copy and paste a formatted citation
Spandidos Publications style
Qi H, Liu T, Liu J, Teng Q, Ma Z, Wang S, Wen S, Zhang C, Ren X, Kong H, Kong H, et al: Thioredoxin1 is a target to attenuate diabetes‑induced RPE cell dysfunction in human ARPE19 cells by alleviating oxidative stress. Mol Med Rep 28: 134, 2023.
APA
Qi, H., Liu, T., Liu, J., Teng, Q., Ma, Z., Wang, S. ... Kong, L. (2023). Thioredoxin1 is a target to attenuate diabetes‑induced RPE cell dysfunction in human ARPE19 cells by alleviating oxidative stress. Molecular Medicine Reports, 28, 134. https://doi.org/10.3892/mmr.2023.13021
MLA
Qi, H., Liu, T., Liu, J., Teng, Q., Ma, Z., Wang, S., Wen, S., Zhang, C., Ren, X., Kong, H., Kong, L."Thioredoxin1 is a target to attenuate diabetes‑induced RPE cell dysfunction in human ARPE19 cells by alleviating oxidative stress". Molecular Medicine Reports 28.1 (2023): 134.
Chicago
Qi, H., Liu, T., Liu, J., Teng, Q., Ma, Z., Wang, S., Wen, S., Zhang, C., Ren, X., Kong, H., Kong, L."Thioredoxin1 is a target to attenuate diabetes‑induced RPE cell dysfunction in human ARPE19 cells by alleviating oxidative stress". Molecular Medicine Reports 28, no. 1 (2023): 134. https://doi.org/10.3892/mmr.2023.13021
Copy and paste a formatted citation
x
Spandidos Publications style
Qi H, Liu T, Liu J, Teng Q, Ma Z, Wang S, Wen S, Zhang C, Ren X, Kong H, Kong H, et al: Thioredoxin1 is a target to attenuate diabetes‑induced RPE cell dysfunction in human ARPE19 cells by alleviating oxidative stress. Mol Med Rep 28: 134, 2023.
APA
Qi, H., Liu, T., Liu, J., Teng, Q., Ma, Z., Wang, S. ... Kong, L. (2023). Thioredoxin1 is a target to attenuate diabetes‑induced RPE cell dysfunction in human ARPE19 cells by alleviating oxidative stress. Molecular Medicine Reports, 28, 134. https://doi.org/10.3892/mmr.2023.13021
MLA
Qi, H., Liu, T., Liu, J., Teng, Q., Ma, Z., Wang, S., Wen, S., Zhang, C., Ren, X., Kong, H., Kong, L."Thioredoxin1 is a target to attenuate diabetes‑induced RPE cell dysfunction in human ARPE19 cells by alleviating oxidative stress". Molecular Medicine Reports 28.1 (2023): 134.
Chicago
Qi, H., Liu, T., Liu, J., Teng, Q., Ma, Z., Wang, S., Wen, S., Zhang, C., Ren, X., Kong, H., Kong, L."Thioredoxin1 is a target to attenuate diabetes‑induced RPE cell dysfunction in human ARPE19 cells by alleviating oxidative stress". Molecular Medicine Reports 28, no. 1 (2023): 134. https://doi.org/10.3892/mmr.2023.13021
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