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Proteomics analysis of lung tissue reveals protein makers for the lung injury of adjuvant arthritis rats

  • Authors:
    • Ping-Heng Zhang
    • Dan-Bin Wu
    • Jian Liu
    • Jian-Ting Wen
    • En-Sheng Chen
    • Chang-Hong Xiao
  • View Affiliations / Copyright

    Affiliations: Rheumatology and Immunology Department, Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510315, P.R. China, Department of Traditional Chinese Medicine, School of Medicine, First Affiliated Hospital, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China, Department of Rheumatology and Immunology, First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei, Anhui 230038, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 163
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    Published online on: July 11, 2023
       https://doi.org/10.3892/mmr.2023.13051
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Abstract

Lung injury is one of the common extra‑articular lesions in rheumatoid arthritis (RA). Due to its insidious onset and no obvious clinical symptoms, it can be easily dismissed in the early stage of diagnosis, which is one of the reasons that leads to a decline of the quality of life and subsequent death of patients with RA. However, its pathogenesis is still unclear and there is a lack of effective therapeutic targets. In the present study, tandem mass tag‑labeled proteomics was used to research the lung tissue proteins in RA model (adjuvant arthritis, AA) rats that had secondary lung injury. The aim of the present study was to identify the differentially expressed proteins related to RA‑lung injury, determine their potential role in the pathogenesis of RA‑lung injury and provide potential targets for clinical treatment. Lung tissue samples were collected from AA‑lung injury and normal rats. The differentially expressed proteins (DEPs) were identified by tandem mass spectrometry. Bioinformatic analysis was used to assess the biological processes and signaling pathways associated with these DEPs. A total of 310 DEPs were found, of which 244 were upregulated and 66 were downregulated. KEGG anlysis showed that ‘fatty acid degradation’, ‘fatty acid metabolism’, ‘fatty acid elongation’, ‘complement and coagulation cascades’, ‘peroxisome proliferator‑activated receptor signaling pathway’ and ‘hypoxia‑inducible factor signaling pathway’ were significantly upregulated in the lung tissues of AA‑lung injury. Immunofluorescence staining confirmed the increased expression of clusterin, serine protease inhibitors and complement 1qc in lung tissue of rats with AA lung injury. In the present study, the results revealed the significance of certain DEPs (for example, C9, C1qc and Clu) in the occurrence and development of RA‑lung injury and provided support through experiments to identify potential biomarkers for the early diagnosis and prevention of RA‑lung injury.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang P, Wu D, Liu J, Wen J, Chen E and Xiao C: Proteomics analysis of lung tissue reveals protein makers for the lung injury of adjuvant arthritis rats. Mol Med Rep 28: 163, 2023.
APA
Zhang, P., Wu, D., Liu, J., Wen, J., Chen, E., & Xiao, C. (2023). Proteomics analysis of lung tissue reveals protein makers for the lung injury of adjuvant arthritis rats. Molecular Medicine Reports, 28, 163. https://doi.org/10.3892/mmr.2023.13051
MLA
Zhang, P., Wu, D., Liu, J., Wen, J., Chen, E., Xiao, C."Proteomics analysis of lung tissue reveals protein makers for the lung injury of adjuvant arthritis rats". Molecular Medicine Reports 28.3 (2023): 163.
Chicago
Zhang, P., Wu, D., Liu, J., Wen, J., Chen, E., Xiao, C."Proteomics analysis of lung tissue reveals protein makers for the lung injury of adjuvant arthritis rats". Molecular Medicine Reports 28, no. 3 (2023): 163. https://doi.org/10.3892/mmr.2023.13051
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang P, Wu D, Liu J, Wen J, Chen E and Xiao C: Proteomics analysis of lung tissue reveals protein makers for the lung injury of adjuvant arthritis rats. Mol Med Rep 28: 163, 2023.
APA
Zhang, P., Wu, D., Liu, J., Wen, J., Chen, E., & Xiao, C. (2023). Proteomics analysis of lung tissue reveals protein makers for the lung injury of adjuvant arthritis rats. Molecular Medicine Reports, 28, 163. https://doi.org/10.3892/mmr.2023.13051
MLA
Zhang, P., Wu, D., Liu, J., Wen, J., Chen, E., Xiao, C."Proteomics analysis of lung tissue reveals protein makers for the lung injury of adjuvant arthritis rats". Molecular Medicine Reports 28.3 (2023): 163.
Chicago
Zhang, P., Wu, D., Liu, J., Wen, J., Chen, E., Xiao, C."Proteomics analysis of lung tissue reveals protein makers for the lung injury of adjuvant arthritis rats". Molecular Medicine Reports 28, no. 3 (2023): 163. https://doi.org/10.3892/mmr.2023.13051
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