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Preventive and ameliorative effects of potato exosomes on UVB‑induced photodamage in keratinocyte HaCaT cells

  • Authors:
    • Yeji Lee
    • Da-Young Jeong
    • Yong Chull Jeun
    • Han Choe
    • Sanghwa Yang
  • View Affiliations / Copyright

    Affiliations: Research & Development Team, Nextab, Inc., Seoul 03908, Republic of Korea, Sustainable Agriculture Research Institute, Jeju National University, Jeju 63234, Republic of Korea, Department of Physiology, Biomedical Institute of Technology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea
    Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 167
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    Published online on: July 13, 2023
       https://doi.org/10.3892/mmr.2023.13054
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Abstract

Exosomes isolated from potato (Solanum tuberosum) exhibit the biophysical characteristics of exosomes observed in mammalian cells and microorganisms, as determined by dynamic light scattering analysis and transmission electron microscopy. In the present study, it was shown that potato exosomes (ExoPs) can penetrate keratinocyte HaCaT cells, as determined by confocal microscopy and flow cytometry. In addition, ExoPs can suppress the expression of the collagen‑destroying enzymes MMP1, 2 and 9, and the inflammatory cytokines IL6 and TNF‑α, while inducing the expression of glutathione S‑transferase α 4, a cellular detoxifying enzyme, as revealed by reverse transcription‑quantitative PCR. Furthermore, ExoPs promote HaCaT cell proliferation, exhibit in vitro antioxidant activity against the free radical 2,2‑diphenyl‑β‑picrylhydrazyl, and protect cells from hydrogen peroxide‑induced cytotoxicity. ExoPs can also minimize the induction of photodamage initiated by ultraviolet B (UVB) irradiation, and have the tendency to cure the photodamage already incurred on cells by UVB irradiation. ExoPs also prevent collagen degradation as observed in the culture media of UVB‑irradiated HaCaT cells. Collectively, ExoPs may protect and ameliorate photodamage in keratinocyte HaCaT cells.
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Copy and paste a formatted citation
Spandidos Publications style
Lee Y, Jeong D, Jeun YC, Choe H and Yang S: Preventive and ameliorative effects of potato exosomes on UVB‑induced photodamage in keratinocyte HaCaT cells. Mol Med Rep 28: 167, 2023.
APA
Lee, Y., Jeong, D., Jeun, Y.C., Choe, H., & Yang, S. (2023). Preventive and ameliorative effects of potato exosomes on UVB‑induced photodamage in keratinocyte HaCaT cells. Molecular Medicine Reports, 28, 167. https://doi.org/10.3892/mmr.2023.13054
MLA
Lee, Y., Jeong, D., Jeun, Y. C., Choe, H., Yang, S."Preventive and ameliorative effects of potato exosomes on UVB‑induced photodamage in keratinocyte HaCaT cells". Molecular Medicine Reports 28.3 (2023): 167.
Chicago
Lee, Y., Jeong, D., Jeun, Y. C., Choe, H., Yang, S."Preventive and ameliorative effects of potato exosomes on UVB‑induced photodamage in keratinocyte HaCaT cells". Molecular Medicine Reports 28, no. 3 (2023): 167. https://doi.org/10.3892/mmr.2023.13054
Copy and paste a formatted citation
x
Spandidos Publications style
Lee Y, Jeong D, Jeun YC, Choe H and Yang S: Preventive and ameliorative effects of potato exosomes on UVB‑induced photodamage in keratinocyte HaCaT cells. Mol Med Rep 28: 167, 2023.
APA
Lee, Y., Jeong, D., Jeun, Y.C., Choe, H., & Yang, S. (2023). Preventive and ameliorative effects of potato exosomes on UVB‑induced photodamage in keratinocyte HaCaT cells. Molecular Medicine Reports, 28, 167. https://doi.org/10.3892/mmr.2023.13054
MLA
Lee, Y., Jeong, D., Jeun, Y. C., Choe, H., Yang, S."Preventive and ameliorative effects of potato exosomes on UVB‑induced photodamage in keratinocyte HaCaT cells". Molecular Medicine Reports 28.3 (2023): 167.
Chicago
Lee, Y., Jeong, D., Jeun, Y. C., Choe, H., Yang, S."Preventive and ameliorative effects of potato exosomes on UVB‑induced photodamage in keratinocyte HaCaT cells". Molecular Medicine Reports 28, no. 3 (2023): 167. https://doi.org/10.3892/mmr.2023.13054
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