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Tripartite motif‑containing 14 may aggravate cardiac hypertrophy via the AKT signalling pathway in neonatal rat cardiomyocytes and transgenic mice

  • Authors:
    • Hongwei Hou
    • Yan Chen
    • Xiuyuan Feng
    • Guang Xu
    • Min Yan
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, The Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310000, P.R. China, Department of Cardiology, Ezhou Central Hospital, Ezhou, Hubei 436000, P.R. China, Department of General Practice, The Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310000, P.R. China
    Copyright: © Hou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 173
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    Published online on: July 27, 2023
       https://doi.org/10.3892/mmr.2023.13060
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Abstract

Tripartite motif‑containing 14 (TRIM14) is an E3 ubiquitin ligase that primarily participates in the natural immune response and in tumour development via ubiquitination. However, the role of TRIM14 in cardiac hypertrophy is not currently clear. The present study examined the role of TRIM14 in cardiac hypertrophy and its potential molecular mechanism. TRIM14 was overexpressed in neonatal rat cardiomyocytes using adenovirus and cardiomyocyte hypertrophy was induced using phenylephrine (PE). Cardiomyocyte hypertrophy was assessed by measuring cardiomyocyte surface area and markers of hypertrophy. In addition, TRIM14‑transgenic (TRIM14‑TG) mice were created and cardiac hypertrophy was induced using transverse aortic constriction (TAC). Cardiac function, heart weight‑to‑body weight ratio (HW/BW), cardiomyocyte cross‑sectional area, cardiac fibrosis and hypertrophic markers were further examined. The expression of AKT signalling pathway‑related proteins was detected. TRIM14 overexpression in cardiomyocytes promoted PE‑induced increases in cardiomyocyte surface area and hypertrophic markers. TRIM14‑TG mice developed worse cardiac function, greater HW/BW, cross‑sectional area and cardiac fibrosis, and higher levels of hypertrophic markers in response to TAC. TRIM14 overexpression also increased the phosphorylation levels of AKT, GSK‑3β, mTOR and p70S6K in vivo and in vitro. To the best our knowledge, the present study was the first to reveal that overexpression of TRIM14 aggravated cardiac hypertrophy in vivo and in vitro, which may be related to activation of the AKT signalling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Hou H, Chen Y, Feng X, Xu G and Yan M: Tripartite motif‑containing 14 may aggravate cardiac hypertrophy via the AKT signalling pathway in neonatal rat cardiomyocytes and transgenic mice. Mol Med Rep 28: 173, 2023.
APA
Hou, H., Chen, Y., Feng, X., Xu, G., & Yan, M. (2023). Tripartite motif‑containing 14 may aggravate cardiac hypertrophy via the AKT signalling pathway in neonatal rat cardiomyocytes and transgenic mice. Molecular Medicine Reports, 28, 173. https://doi.org/10.3892/mmr.2023.13060
MLA
Hou, H., Chen, Y., Feng, X., Xu, G., Yan, M."Tripartite motif‑containing 14 may aggravate cardiac hypertrophy via the AKT signalling pathway in neonatal rat cardiomyocytes and transgenic mice". Molecular Medicine Reports 28.3 (2023): 173.
Chicago
Hou, H., Chen, Y., Feng, X., Xu, G., Yan, M."Tripartite motif‑containing 14 may aggravate cardiac hypertrophy via the AKT signalling pathway in neonatal rat cardiomyocytes and transgenic mice". Molecular Medicine Reports 28, no. 3 (2023): 173. https://doi.org/10.3892/mmr.2023.13060
Copy and paste a formatted citation
x
Spandidos Publications style
Hou H, Chen Y, Feng X, Xu G and Yan M: Tripartite motif‑containing 14 may aggravate cardiac hypertrophy via the AKT signalling pathway in neonatal rat cardiomyocytes and transgenic mice. Mol Med Rep 28: 173, 2023.
APA
Hou, H., Chen, Y., Feng, X., Xu, G., & Yan, M. (2023). Tripartite motif‑containing 14 may aggravate cardiac hypertrophy via the AKT signalling pathway in neonatal rat cardiomyocytes and transgenic mice. Molecular Medicine Reports, 28, 173. https://doi.org/10.3892/mmr.2023.13060
MLA
Hou, H., Chen, Y., Feng, X., Xu, G., Yan, M."Tripartite motif‑containing 14 may aggravate cardiac hypertrophy via the AKT signalling pathway in neonatal rat cardiomyocytes and transgenic mice". Molecular Medicine Reports 28.3 (2023): 173.
Chicago
Hou, H., Chen, Y., Feng, X., Xu, G., Yan, M."Tripartite motif‑containing 14 may aggravate cardiac hypertrophy via the AKT signalling pathway in neonatal rat cardiomyocytes and transgenic mice". Molecular Medicine Reports 28, no. 3 (2023): 173. https://doi.org/10.3892/mmr.2023.13060
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