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Article

Novel anti‑hepatitis B virus flavonoids sakuranetin and velutin from Rhus retinorrhoea

  • Authors:
    • Sarfaraz Ahmed
    • Mohammad K. Parvez
    • Mohammed S. Al-Dosari
    • Mazin A.S. Abdelwahid
    • Tawfeq A. Alhowiriny
    • Adnan J. Al-Rehaily
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia, Department of Organic Chemistry, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980‑8678, Japan
  • Article Number: 176
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    Published online on: August 1, 2023
       https://doi.org/10.3892/mmr.2023.13063
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Abstract

Drug‑resistance in hepatitis B virus (HBV), especially due to prolonged treatment with nucleoside analogs, such as lamivudine (LAM), remains a clinical challenge. Alternatively, several plant products and isolated phytochemicals have been used as promising anti‑HBV therapeutics with no sign of resistance. Among all known Rhus species, R. coriaria, R. succedanea and R. tripartite have been widely studied for their anti‑HBV efficacy, however, the effects of R. retinorrhoea have not been previously investigated. The current study reported the isolation of two flavonoids, namely sakuranetin (SEK) and velutin (VEL), from the dichloromethane fraction of R. retinorrhoea aerial parts using chromatography and spectral analyses. The two flavonoids (6.25‑50 µg/ml) were pre‑tested for non‑hepatocytotoxicity using an MTT assay and their dose‑ and time‑dependent inhibitory activities against HBV [hepatitis B surface antigen (HBsAg) and hepatitis B ‘e’ antigen (HBeAg)] in cultured HepG2.2.15 cells were assessed by ELISA. SEK and VEL at the selected doses (12.5 µg/ml) significantly inhibited HBsAg by ~58.8 and ~56.4%, respectively, and HBeAg by ~55.5 and ~52.4%, respectively, on day 5. The reference drugs LAM and quercetin (anti‑HBV flavonoids), suppressed the production of HBsAg/HBeAg by ~86.4/~64 and ~84.5/~62%, respectively. Furthermore, molecular docking of the flavonoids with HBV polymerase and capsid proteins revealed the formation of stable complexes with good docking energies, thus supporting their structure‑based antiviral mechanism. In conclusion, the present study was the first to demonstrate the anti‑HBV therapeutic activities of SEK and VEL isolated from R. retinorrhoea.
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Copy and paste a formatted citation
Spandidos Publications style
Ahmed S, Parvez MK, Al-Dosari MS, Abdelwahid MA, Alhowiriny TA and Al-Rehaily AJ: Novel anti‑hepatitis B virus flavonoids sakuranetin and velutin from<em> Rhus retinorrhoea</em>. Mol Med Rep 28: 176, 2023.
APA
Ahmed, S., Parvez, M.K., Al-Dosari, M.S., Abdelwahid, M.A., Alhowiriny, T.A., & Al-Rehaily, A.J. (2023). Novel anti‑hepatitis B virus flavonoids sakuranetin and velutin from<em> Rhus retinorrhoea</em>. Molecular Medicine Reports, 28, 176. https://doi.org/10.3892/mmr.2023.13063
MLA
Ahmed, S., Parvez, M. K., Al-Dosari, M. S., Abdelwahid, M. A., Alhowiriny, T. A., Al-Rehaily, A. J."Novel anti‑hepatitis B virus flavonoids sakuranetin and velutin from<em> Rhus retinorrhoea</em>". Molecular Medicine Reports 28.3 (2023): 176.
Chicago
Ahmed, S., Parvez, M. K., Al-Dosari, M. S., Abdelwahid, M. A., Alhowiriny, T. A., Al-Rehaily, A. J."Novel anti‑hepatitis B virus flavonoids sakuranetin and velutin from<em> Rhus retinorrhoea</em>". Molecular Medicine Reports 28, no. 3 (2023): 176. https://doi.org/10.3892/mmr.2023.13063
Copy and paste a formatted citation
x
Spandidos Publications style
Ahmed S, Parvez MK, Al-Dosari MS, Abdelwahid MA, Alhowiriny TA and Al-Rehaily AJ: Novel anti‑hepatitis B virus flavonoids sakuranetin and velutin from<em> Rhus retinorrhoea</em>. Mol Med Rep 28: 176, 2023.
APA
Ahmed, S., Parvez, M.K., Al-Dosari, M.S., Abdelwahid, M.A., Alhowiriny, T.A., & Al-Rehaily, A.J. (2023). Novel anti‑hepatitis B virus flavonoids sakuranetin and velutin from<em> Rhus retinorrhoea</em>. Molecular Medicine Reports, 28, 176. https://doi.org/10.3892/mmr.2023.13063
MLA
Ahmed, S., Parvez, M. K., Al-Dosari, M. S., Abdelwahid, M. A., Alhowiriny, T. A., Al-Rehaily, A. J."Novel anti‑hepatitis B virus flavonoids sakuranetin and velutin from<em> Rhus retinorrhoea</em>". Molecular Medicine Reports 28.3 (2023): 176.
Chicago
Ahmed, S., Parvez, M. K., Al-Dosari, M. S., Abdelwahid, M. A., Alhowiriny, T. A., Al-Rehaily, A. J."Novel anti‑hepatitis B virus flavonoids sakuranetin and velutin from<em> Rhus retinorrhoea</em>". Molecular Medicine Reports 28, no. 3 (2023): 176. https://doi.org/10.3892/mmr.2023.13063
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