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Knockdown of ANLN inhibits the progression of lung adenocarcinoma via pyroptosis activation

  • Authors:
    • Li Sheng
    • Yanhai Kang
    • Denglin Chen
    • Linyang Shi
  • View Affiliations / Copyright

    Affiliations: Department of Medical Oncology, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, Hainan 570311, P.R. China, Department of Psychology, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, Hainan 570311, P.R. China
    Copyright: © Sheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 177
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    Published online on: August 3, 2023
       https://doi.org/10.3892/mmr.2023.13064
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Abstract

Significant advancements have been achieved in the area of molecular targeted therapy for lung adenocarcinoma (LUAD). However, the complex molecular patterns and high heterogeneity of LUAD confine the efficacy of these therapies to a specific subset of patients; therefore, it is necessary to explore novel targets for LUAD treatment. The expression levels of anillin (ANLN) in LUAD were analyzed using the Gene Expression Profiling Interactive Analysis database. Furthermore, the association between ANLN gene expression and patient survival outcomes was evaluated using the Kaplan‑Meier Plotter. Subsequently, small interfering RNA (siRNA) transfection was performed to knock down ANLN in A549 and H1299 cell lines, after which, TUNEL, colony formation and Transwell assays were conducted to assess cell death, colony formation and migration, respectively. Additionally, western blot analysis was performed to analyze the expression levels of caspase‑1, interleukin (IL)‑18 (IL‑18), IL‑1β, NLR family pyrin domain‑containing 3 (NLRP3), apoptosis‑associated speck‑like protein containing a CARD domain (ASC) and cleaved gasdermin D (GSDMD) following ANLN knockdown. The results revealed that ANLN mRNA expression was significantly increased in LUAD tissues compared with adjacent normal samples. Furthermore, the expression levels of ANLN displayed an increasing trend with advancing clinical stage. Furthermore, patients with high ANLN expression levels exhibited poor overall survival rates compared with those with low ANLN expression levels. Subsequent ANLN knockdown experiments indicated elevated cell death rate, and reduced colony formation and migration in both A549 and H1299 cells. Additionally, ANLN knockdown resulted in increased protein expression levels of pyroptosis‑associated molecules, including caspase‑1, NLRP3, cleaved‑GSDMD, IL‑1β, ASC and IL‑18 in both A549 and H1299 cells. In conclusion, ANLN represents an important gene and a promising therapeutic target for LUAD. Its potential as a therapeutic target makes it an interesting candidate for further exploration in the development of novel treatment strategies for LUAD.
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Spandidos Publications style
Sheng L, Kang Y, Chen D and Shi L: Knockdown of ANLN inhibits the progression of lung adenocarcinoma via pyroptosis activation. Mol Med Rep 28: 177, 2023.
APA
Sheng, L., Kang, Y., Chen, D., & Shi, L. (2023). Knockdown of ANLN inhibits the progression of lung adenocarcinoma via pyroptosis activation. Molecular Medicine Reports, 28, 177. https://doi.org/10.3892/mmr.2023.13064
MLA
Sheng, L., Kang, Y., Chen, D., Shi, L."Knockdown of ANLN inhibits the progression of lung adenocarcinoma via pyroptosis activation". Molecular Medicine Reports 28.3 (2023): 177.
Chicago
Sheng, L., Kang, Y., Chen, D., Shi, L."Knockdown of ANLN inhibits the progression of lung adenocarcinoma via pyroptosis activation". Molecular Medicine Reports 28, no. 3 (2023): 177. https://doi.org/10.3892/mmr.2023.13064
Copy and paste a formatted citation
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Spandidos Publications style
Sheng L, Kang Y, Chen D and Shi L: Knockdown of ANLN inhibits the progression of lung adenocarcinoma via pyroptosis activation. Mol Med Rep 28: 177, 2023.
APA
Sheng, L., Kang, Y., Chen, D., & Shi, L. (2023). Knockdown of ANLN inhibits the progression of lung adenocarcinoma via pyroptosis activation. Molecular Medicine Reports, 28, 177. https://doi.org/10.3892/mmr.2023.13064
MLA
Sheng, L., Kang, Y., Chen, D., Shi, L."Knockdown of ANLN inhibits the progression of lung adenocarcinoma via pyroptosis activation". Molecular Medicine Reports 28.3 (2023): 177.
Chicago
Sheng, L., Kang, Y., Chen, D., Shi, L."Knockdown of ANLN inhibits the progression of lung adenocarcinoma via pyroptosis activation". Molecular Medicine Reports 28, no. 3 (2023): 177. https://doi.org/10.3892/mmr.2023.13064
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