Open Access

Metabolomic profiling of a neurodegenerative retina following optic nerve transection

  • Authors:
    • Jun-Ya Zhu
    • Xi-Sen Ni
    • Xiao-Yan Han
    • Sha Liu
    • Yu-Ke Ji
    • Jin Yao
    • Biao Yan
  • View Affiliations

  • Published online on: August 3, 2023     https://doi.org/10.3892/mmr.2023.13065
  • Article Number: 178
  • Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The degeneration of retinal ganglion cells (RGCs) often causes irreversible vision impairment. Prevention of RGC degeneration can prevent or delay the deterioration of visual function. The present study aimed to investigate retinal metabolic profiles following optic nerve transection (ONT) injury and identify the potential metabolic targets for the prevention of RGC degeneration. Retinal samples were dissected from ONT group and non‑ONT group. The untargeted metabolomics were carried out using liquid chromatography‑tandem mass spectrometry. The involved pathways and biomarkers were analyzed using Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and MetaboAnalyst 5.0. In the ONT group, 689 disparate metabolites were detected, including lipids and lipid‑like molecules. A total of 122 metabolites were successfully annotated and enriched in 50 KEGG pathways. Among them, ‘sphingolipid metabolism’ and ‘primary bile acid biosynthesis’ were identified involved in RGC degeneration. A total of five metabolites were selected as the candidate biomarkers for detecting RGC degeneration with an AUC value of 1. The present study revealed that lipid‑related metabolism was involved in the pathogenesis of retinal neurodegeneration. Taurine, taurochenodesoxycholic acid, taurocholic acid (TCA), sphingosine, and galabiosylceramide are shown as the promising biomarkers for the diagnosis of RGC degeneration.
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September-2023
Volume 28 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhu J, Ni X, Han X, Liu S, Ji Y, Yao J and Yan B: Metabolomic profiling of a neurodegenerative retina following optic nerve transection. Mol Med Rep 28: 178, 2023
APA
Zhu, J., Ni, X., Han, X., Liu, S., Ji, Y., Yao, J., & Yan, B. (2023). Metabolomic profiling of a neurodegenerative retina following optic nerve transection. Molecular Medicine Reports, 28, 178. https://doi.org/10.3892/mmr.2023.13065
MLA
Zhu, J., Ni, X., Han, X., Liu, S., Ji, Y., Yao, J., Yan, B."Metabolomic profiling of a neurodegenerative retina following optic nerve transection". Molecular Medicine Reports 28.3 (2023): 178.
Chicago
Zhu, J., Ni, X., Han, X., Liu, S., Ji, Y., Yao, J., Yan, B."Metabolomic profiling of a neurodegenerative retina following optic nerve transection". Molecular Medicine Reports 28, no. 3 (2023): 178. https://doi.org/10.3892/mmr.2023.13065