Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
October-2023 Volume 28 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2023 Volume 28 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Serum levels of vault RNA significantly varied in patients with haematological malignancies

  • Authors:
    • Haruka Kato
    • Yuki Hashimoto
    • Yuki Hatayama
    • Hisashi Shimohiro
    • Toru Motokura
  • View Affiliations / Copyright

    Affiliations: Department of Pathobiological Science and Technology, School of Health Science, Tottori University Faculty of Medicine, Yonago, Tottori 683‑8503, Japan, Division of Clinical Laboratory, Tottori University Hospital, Yonago, Tottori 683‑8504, Japan, Division of Clinical Laboratory Medicine, Department of Multidisciplinary Internal Medicine, Tottori University Faculty of Medicine, Yonago, Tottori 683‑8503, Japan
  • Article Number: 190
    |
    Published online on: August 28, 2023
       https://doi.org/10.3892/mmr.2023.13077
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Among extracellular non‑coding RNAs, serum levels of microRNAs have been extensively investigated in cancers. In contrast, the serum levels of vault RNAs (vtRNAs) in relation to various disease conditions remain poorly understood. The present study evaluated the clinical significance of serum vtRNA1‑1 levels in patients with blood diseases. The stability and sub‑localisation of serum vtRNA1‑1 was assessed and a reverse transcription‑quantitative PCR method using spiked RNA to quantify serum vtRNA1‑1 was developed. Serum vtRNA1‑1 levels were assessed in 102 individuals with blood diseases. Serum vtRNA1‑1 was demonstrated to be stable for three weeks at 4˚C and was not confined to the exosome fractions. Spiking RNA was used to correct for the inconsistency in RNA extraction. The serum vtRNA1‑1 levels ranged between 7.28 and 8.76 log10 cps/ml (median 8.05) in control individuals (n=46). Serum vtRNA1‑1 levels correlated with leukocyte counts and increased to a maximum of 10.01 log10 cps/ml in patients with bulky leukaemia and lymphoma and decreased to 6.52 log10 cps/ml during intensive chemotherapy. The serum vtRNA1‑1 levels varied significantly in patients with haematological malignancies. Serum vtRNA1‑1 may originate from haematological cells and are a potential biomarker of normal and malignant haematological activities.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Kedersha NL and Rome LH: Isolation and characterization of a novel ribonucleoprotein particle: Large structures contain a single species of small RNA. J Cell Biol. 103:699–709. 1986. View Article : Google Scholar : PubMed/NCBI

2 

Stadler PF, Chen JJ, Hackermüller J, Hoffmann S, Horn F, Khaitovich P, Kretzschmar AK, Mosig A, Prohaska SJ, Qi X, et al: Evolution of vault RNAs. Mol Biol Evol. 26:1975–1991. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Kickhoefer VA, Searles RP, Kedersha NL, Garber ME, Johnson DL and Rome LH: Vault ribonucleoprotein particles from rat and bullfrog contain a related small RNA that is transcribed by RNA polymerase III. J Biol Chem. 268:7868–7873. 1993. View Article : Google Scholar : PubMed/NCBI

4 

Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, et al: A mammalian microRNA expression atlas based on small RNA library sequencing. Cell. 129:1401–1414. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Lee K, Kunkeaw N, Jeon SH, Lee I, Johnson BH, Kang GY, Bang JY, Park HS, Leelayuwat C and Lee YS: Precursor miR-886, a novel noncoding RNA repressed in cancer, associates with PKR and modulates its activity. RNA. 17:1076–1089. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Nandy C, Mrázek J, Stoiber H, Grässer FA, Hüttenhofer A and Polacek N: Epstein-Barr virus-induced expression of a novel human vault RNA. J Mol Biol. 388:776–784. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Li F, Chen Y, Zhang Z, Ouyang J, Wang Y, Yan R, Huang S, Gao GF, Guo G and Chen JL: Robust expression of vault RNAs induced by influenza A virus plays a critical role in suppression of PKR-mediated innate immunity. Nucleic Acids Res. 43:10321–10337. 2015.PubMed/NCBI

8 

Amort M, Nachbauer B, Tuzlak S, Kieser A, Schepers A, Villunger A and Polacek N: Expression of the vault RNA protects cells from undergoing apoptosis. Nat Commun. 6:70302015. View Article : Google Scholar : PubMed/NCBI

9 

Gopinath SC, Wadhwa R and Kumar PK: Expression of noncoding vault RNA in human malignant cells and its importance in mitoxantrone resistance. Mol Cancer Res. 8:1536–1546. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Chen J, OuYang H, An X and Liu S: Vault RNAs partially induces drug resistance of human tumor cells MCF-7 by binding to the RNA/DNA-binding protein PSF and inducing oncogene GAGE6. PLoS One. 13:e01913252018. View Article : Google Scholar : PubMed/NCBI

11 

Bracher L, Ferro I, Pulido-Quetglas C, Ruepp MD, Johnson R and Polacek N: Human vtRNA1-1 levels modulate signaling pathways and regulate apoptosis in human cancer cells. Biomolecules. 10:6142020. View Article : Google Scholar : PubMed/NCBI

12 

Ferro I, Gavini J, Gallo S, Bracher L, Landolfo M, Candinas D, Stroka DM and Polacek N: The human vault RNA enhances tumorigenesis and chemoresistance through the lysosome in hepatocellular carcinoma. Autophagy. 18:191–203. 2022. View Article : Google Scholar : PubMed/NCBI

13 

Horos R, Buscher M, Kleinendorst R, Alleaume AM, Tarafder AK, Schwarzl T, Dziuba D, Tischer C, Zielonka EM, Adak A, et al: The small non-coding vault RNA1-1 acts as a riboregulator of autophagy. Cell. 176:1054–1067. e122019. View Article : Google Scholar : PubMed/NCBI

14 

Buscher M, Horos R, Huppertz I, Haubrich K, Dobrev N, Baudin F, Hennig J and Hentze MW: Vault RNA1-1 riboregulates the autophagic function of p62 by binding to lysine 7 and arginine 21, both of which are critical for p62 oligomerization. RNA. 28:742–755. 2022. View Article : Google Scholar : PubMed/NCBI

15 

Gallo S, Kong E, Ferro I and Polacek N: Small but powerful: The human vault RNAs as multifaceted modulators of pro-survival characteristics and tumorigenesis. Cancers (Basel). 14:27872022. View Article : Google Scholar : PubMed/NCBI

16 

Nolte-'t Hoen EN, Buermans HP, Waasdorp M, Stoorvogel W, Wauben MH and 't Hoen PA: Deep sequencing of RNA from immune cell-derived vesicles uncovers the selective incorporation of small non-coding RNA biotypes with potential regulatory functions. Nucleic Acids Res. 40:9272–9285. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Shurtleff MJ, Yao J, Qin Y, Nottingham RM, Temoche-Diaz MM, Schekman R and Lambowitz AM: Broad role for YBX1 in defining the small noncoding RNA composition of exosomes. Proc Natl Acad Sci USA. 114:E8987–E8995. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Ramayanti O, Verkuijlen SAWM, Novianti P, Scheepbouwer C, Misovic B, Koppers-Lalic D, van Weering J, Beckers L, Adham M, Martorelli D, et al: Vesicle-bound EBV-BART13-3p miRNA in circulation distinguishes nasopharyngeal from other head and neck cancer and asymptomatic EBV-infections. Int J Cancer. 144:2555–2566. 2019. View Article : Google Scholar : PubMed/NCBI

19 

Nakai W, Yoshida T, Diez D, Miyatake Y, Nishibu T, Imawaka N, Naruse K, Sadamura Y and Hanayama R: A novel affinity-based method for the isolation of highly purified extracellular vesicles. Sci Rep. 6:339352016. View Article : Google Scholar : PubMed/NCBI

20 

Kanda Y: Investigation of the freely available easy-to-use software ‘EZR’ for medical statistics. Bone Marrow Transplant. 48:452–458. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Motokura T, Kobayashi Y, Fujita A, Nakamura Y, Taniguchi T, Uchimaru K and Asano S: Clinical significance of serial measurement of the serum levels of soluble interleukin-2 receptor and soluble CD8 in malignant lymphoma. Leuk Lymphoma. 16:355–362. 1995. View Article : Google Scholar : PubMed/NCBI

22 

Jeppesen DK, Fenix AM, Franklin JL, Higginbotham JN, Zhang Q, Zimmerman LJ, Liebler DC, Ping J, Liu Q, Evans R, et al: Reassessment of exosome composition. Cell. 177:428–445. e182019. View Article : Google Scholar : PubMed/NCBI

23 

Schneider RJ, Seibert K, Passe S, Little C, Gee T, Lee BJ III, Miké V and Young CW: Prognostic significance of serum lactate dehydrogenase in malignant lymphoma. Cancer. 46:149–143. 1980. View Article : Google Scholar

24 

Geva M, Pryce A, Shouval R, Fein JA, Danylesko I, Shem-Tov N, Yerushalmi R, Shimoni A, Szydlo R, Pavlu J and Nagler A: High lactate dehydrogenase at time of admission for allogeneic hematopoietic transplantation associates to poor survival in acute myeloid leukemia and non-Hodgkin lymphoma. Bone Marrow Transplamt. 56:2690–2696. 2021. View Article : Google Scholar : PubMed/NCBI

25 

Tilly H, Castaigne S, Bordessoule D, Casassus P, Le Prisé PY, Tertian G, Desablens B, Henry-Amar M and Degos L: Low-dose cytarabine versus intensive chemotherapy in the treatment of acute nonlymphocytic leukemia in the elderly. J Clin Oncol. 8:272–279. 1990. View Article : Google Scholar : PubMed/NCBI

26 

Carella AM, Podesta M, Frassoni F, Raffo MR, Pollicardo N, Pungolino E, Vimercati R, Sessarego M, Parodi C, Rabitti C, et al: Collection of ‘normal’ blood repopulating cells during early hemopoietic recovery after intensive conventional chemotherapy in chronic myelogenous leukemia. Bone Marrow Transplant. 12:267–271. 1993.PubMed/NCBI

27 

Du Bois D and Du Bois EF: A formula to estimate the approximate surface area if height and weight be known. Arch Intern Med. 17:863–871. 1916. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Kato H, Hashimoto Y, Hatayama Y, Shimohiro H and Motokura T: Serum levels of vault RNA significantly varied in patients with haematological malignancies. Mol Med Rep 28: 190, 2023.
APA
Kato, H., Hashimoto, Y., Hatayama, Y., Shimohiro, H., & Motokura, T. (2023). Serum levels of vault RNA significantly varied in patients with haematological malignancies. Molecular Medicine Reports, 28, 190. https://doi.org/10.3892/mmr.2023.13077
MLA
Kato, H., Hashimoto, Y., Hatayama, Y., Shimohiro, H., Motokura, T."Serum levels of vault RNA significantly varied in patients with haematological malignancies". Molecular Medicine Reports 28.4 (2023): 190.
Chicago
Kato, H., Hashimoto, Y., Hatayama, Y., Shimohiro, H., Motokura, T."Serum levels of vault RNA significantly varied in patients with haematological malignancies". Molecular Medicine Reports 28, no. 4 (2023): 190. https://doi.org/10.3892/mmr.2023.13077
Copy and paste a formatted citation
x
Spandidos Publications style
Kato H, Hashimoto Y, Hatayama Y, Shimohiro H and Motokura T: Serum levels of vault RNA significantly varied in patients with haematological malignancies. Mol Med Rep 28: 190, 2023.
APA
Kato, H., Hashimoto, Y., Hatayama, Y., Shimohiro, H., & Motokura, T. (2023). Serum levels of vault RNA significantly varied in patients with haematological malignancies. Molecular Medicine Reports, 28, 190. https://doi.org/10.3892/mmr.2023.13077
MLA
Kato, H., Hashimoto, Y., Hatayama, Y., Shimohiro, H., Motokura, T."Serum levels of vault RNA significantly varied in patients with haematological malignancies". Molecular Medicine Reports 28.4 (2023): 190.
Chicago
Kato, H., Hashimoto, Y., Hatayama, Y., Shimohiro, H., Motokura, T."Serum levels of vault RNA significantly varied in patients with haematological malignancies". Molecular Medicine Reports 28, no. 4 (2023): 190. https://doi.org/10.3892/mmr.2023.13077
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team