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Fibroblast growth factor 23 regulates hypoxia‑induced osteoblast apoptosis through the autophagy‑signaling pathway

  • Authors:
    • Qipu Yin
    • Hongxia Yang
    • Lun Fang
    • Qi Wu
    • Shan Gao
    • Yadi Wu
    • Lu Zhou
  • View Affiliations / Copyright

    Affiliations: Institute of Sports Medicine, Shandong First Medical University and Shandong Academy of Medical Sciences, Taian, Shandong 271016, P.R. China, School of Nursing, Shandong First Medical University and Shandong Academy of Medical Sciences, Taian, Shandong 271016, P.R. China, Department of Rehabilitation and Physiotherapy, Taian Maternal and Child Health Hospital, Taian, Shandong 271000, P.R. China, School of Pharmaceutical Science, Shandong First Medical University and Shandong Academy of Medical Sciences, Taian, Shandong 271016, P.R. China
    Copyright: © Yin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 199
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    Published online on: September 8, 2023
       https://doi.org/10.3892/mmr.2023.13086
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Abstract

Hypoxia can lead to programmed osteoblast death. Prevention of osteoblast apoptosis caused by hypoxia is of great significance in the study of the occurrence and development of bone necrosis. The present study aimed to investigate the effects and mechanism of fibroblast growth factor 23 (FGF23) on hypoxia‑induced apoptosis in primary osteoblasts and MC3T3‑E1 cells osteoblasts. Cells were transfected with a plasmid carrying the FGF23 gene and a cell model of hypoxia‑induced apoptosis was established. FGF23 mRNA levels were measured using reverse transcription‑quantitative (RT‑q) PCR and western blotting was used to assess protein levels. Apoptosis was analyzed by MTT assay, fluorescein diacetate and ethidium bromide staining, flow cytometry and RT‑qPCR and western blotting were used to verify the mRNA and protein levels of apoptosis‑ and autophagy‑related gene mRNA. The targeted relationship between miR‑17‑5p and FGF23 was confirmed using the StarBase database, TargetScan database and a luciferase reporter assay. FGF23 decreased cell survival and increased the rate of apoptosis. The mRNA and protein expression of the pro‑apoptotic genes Bax and caspases 3 and 9 increased, whereas that of the anti‑apoptotic Bcl‑2 decreased. The expressions of the autophagy‑associated proteins beclin‑1, light chain 3‑II (LC3‑II) and the LC3‑II/LC3‑I ratio were significantly increased. In addition, a luciferase reporter assay confirmed that FGF23 directly regulated micro RNA (miR)‑17‑5p. The effects of FGF23 silencing were reversed by miR‑17‑5p inhibition. FGF23 may regulate hypoxia‑induced osteoblast apoptosis by targeting miR‑17‑5p through the autophagy‑signaling pathway. This provides a rationale for FGF23 as a potential therapeutic target for osteonecrosis of the femoral head.
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Copy and paste a formatted citation
Spandidos Publications style
Yin Q, Yang H, Fang L, Wu Q, Gao S, Wu Y and Zhou L: Fibroblast growth factor 23 regulates hypoxia‑induced osteoblast apoptosis through the autophagy‑signaling pathway. Mol Med Rep 28: 199, 2023.
APA
Yin, Q., Yang, H., Fang, L., Wu, Q., Gao, S., Wu, Y., & Zhou, L. (2023). Fibroblast growth factor 23 regulates hypoxia‑induced osteoblast apoptosis through the autophagy‑signaling pathway. Molecular Medicine Reports, 28, 199. https://doi.org/10.3892/mmr.2023.13086
MLA
Yin, Q., Yang, H., Fang, L., Wu, Q., Gao, S., Wu, Y., Zhou, L."Fibroblast growth factor 23 regulates hypoxia‑induced osteoblast apoptosis through the autophagy‑signaling pathway". Molecular Medicine Reports 28.5 (2023): 199.
Chicago
Yin, Q., Yang, H., Fang, L., Wu, Q., Gao, S., Wu, Y., Zhou, L."Fibroblast growth factor 23 regulates hypoxia‑induced osteoblast apoptosis through the autophagy‑signaling pathway". Molecular Medicine Reports 28, no. 5 (2023): 199. https://doi.org/10.3892/mmr.2023.13086
Copy and paste a formatted citation
x
Spandidos Publications style
Yin Q, Yang H, Fang L, Wu Q, Gao S, Wu Y and Zhou L: Fibroblast growth factor 23 regulates hypoxia‑induced osteoblast apoptosis through the autophagy‑signaling pathway. Mol Med Rep 28: 199, 2023.
APA
Yin, Q., Yang, H., Fang, L., Wu, Q., Gao, S., Wu, Y., & Zhou, L. (2023). Fibroblast growth factor 23 regulates hypoxia‑induced osteoblast apoptosis through the autophagy‑signaling pathway. Molecular Medicine Reports, 28, 199. https://doi.org/10.3892/mmr.2023.13086
MLA
Yin, Q., Yang, H., Fang, L., Wu, Q., Gao, S., Wu, Y., Zhou, L."Fibroblast growth factor 23 regulates hypoxia‑induced osteoblast apoptosis through the autophagy‑signaling pathway". Molecular Medicine Reports 28.5 (2023): 199.
Chicago
Yin, Q., Yang, H., Fang, L., Wu, Q., Gao, S., Wu, Y., Zhou, L."Fibroblast growth factor 23 regulates hypoxia‑induced osteoblast apoptosis through the autophagy‑signaling pathway". Molecular Medicine Reports 28, no. 5 (2023): 199. https://doi.org/10.3892/mmr.2023.13086
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