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Article

Inhibition of the mitochondrial respiratory chain reduces catecholamine‑stimulated lipolysis via increasing lactate production in 3T3‑L1 adipocytes

  • Authors:
    • Nodoka Takeuchi
    • Kazuhiko Higashida
    • Naoya Nakai
  • View Affiliations / Copyright

    Affiliations: Laboratory of Exercise Nutrition, Department of Nutrition, University of Shiga Prefecture, Hikone, Shiga 522‑8533, Japan
  • Article Number: 229
    |
    Published online on: October 19, 2023
       https://doi.org/10.3892/mmr.2023.13116
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Abstract

Adipose tissue serves a significant role in the regulation of energy metabolism in the body. The re‑esterification of the fatty acids generated during lipolysis is critical for efficient lipolysis. However, the effect of the intracellular energy state on lipolytic activity and fatty acid re‑esterification during lipolysis is not yet fully understood. The present study aimed to assess the effect of the intracellular energy state on lipolytic activity and fatty acid re‑esterification during lipolysis. 3T3‑L1 adipocytes were incubated with mitochondrial respiratory chain inhibitors, oligomycin A or rotenone, during isoproterenol stimulation; and glycerol, glucose and lactate concentrations in the medium were measured. Western blot analysis was performed to examine the phosphorylation levels of cAMP‑dependent protein kinase A (PKA). The results showed that inhibition of mitochondrial ATP synthesis decreased catecholamine‑stimulated lipolysis without affecting PKA signaling. The inhibition of mitochondrial respiration increased glucose uptake and lactate production, indicating that a large amount of glucose taken up into the cell was preferentially used for ATP production rather than for re‑esterification. In conclusion, the results of the present study suggested that the energy state during lipolysis may influence lipolytic activity by suppressing fatty acid re‑esterification.
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Copy and paste a formatted citation
Spandidos Publications style
Takeuchi N, Higashida K and Nakai N: Inhibition of the mitochondrial respiratory chain reduces catecholamine‑stimulated lipolysis via increasing lactate production in 3T3‑L1 adipocytes. Mol Med Rep 28: 229, 2023.
APA
Takeuchi, N., Higashida, K., & Nakai, N. (2023). Inhibition of the mitochondrial respiratory chain reduces catecholamine‑stimulated lipolysis via increasing lactate production in 3T3‑L1 adipocytes. Molecular Medicine Reports, 28, 229. https://doi.org/10.3892/mmr.2023.13116
MLA
Takeuchi, N., Higashida, K., Nakai, N."Inhibition of the mitochondrial respiratory chain reduces catecholamine‑stimulated lipolysis via increasing lactate production in 3T3‑L1 adipocytes". Molecular Medicine Reports 28.6 (2023): 229.
Chicago
Takeuchi, N., Higashida, K., Nakai, N."Inhibition of the mitochondrial respiratory chain reduces catecholamine‑stimulated lipolysis via increasing lactate production in 3T3‑L1 adipocytes". Molecular Medicine Reports 28, no. 6 (2023): 229. https://doi.org/10.3892/mmr.2023.13116
Copy and paste a formatted citation
x
Spandidos Publications style
Takeuchi N, Higashida K and Nakai N: Inhibition of the mitochondrial respiratory chain reduces catecholamine‑stimulated lipolysis via increasing lactate production in 3T3‑L1 adipocytes. Mol Med Rep 28: 229, 2023.
APA
Takeuchi, N., Higashida, K., & Nakai, N. (2023). Inhibition of the mitochondrial respiratory chain reduces catecholamine‑stimulated lipolysis via increasing lactate production in 3T3‑L1 adipocytes. Molecular Medicine Reports, 28, 229. https://doi.org/10.3892/mmr.2023.13116
MLA
Takeuchi, N., Higashida, K., Nakai, N."Inhibition of the mitochondrial respiratory chain reduces catecholamine‑stimulated lipolysis via increasing lactate production in 3T3‑L1 adipocytes". Molecular Medicine Reports 28.6 (2023): 229.
Chicago
Takeuchi, N., Higashida, K., Nakai, N."Inhibition of the mitochondrial respiratory chain reduces catecholamine‑stimulated lipolysis via increasing lactate production in 3T3‑L1 adipocytes". Molecular Medicine Reports 28, no. 6 (2023): 229. https://doi.org/10.3892/mmr.2023.13116
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