Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
December-2023 Volume 28 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2023 Volume 28 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Neferine exerts anti‑inflammatory activity in BV‑2 microglial cells and protects mice with MPTP‑induced Parkinson's disease by inhibiting NF‑κB activation

  • Authors:
    • Ting Li
    • Yun Xin Zhai
    • Tianyi Zheng
    • Bing Xu
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, The First People's Hospital of Shenyang, Shenyang, Liaoning 110000, P.R. China, Department of Anal Disease, Shenyang Anorectal Hospital, Shenyang, Liaoning 110000, P.R. China
  • Article Number: 235
    |
    Published online on: November 2, 2023
       https://doi.org/10.3892/mmr.2023.13122
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Abnormal activation of microglia and the production of proinflammatory cytokines can lead to chronic neuroinflammation, which is an important pathological characteristic of Parkinson's disease (PD). Neferine is a chemical compound extracted from lotus seed which has previously been reported to exert protective effects on the development of several types of cancer, myocardial injury and hypoxic‑ischemic encephalopathy. However, its effect on microglial functions in neuroinflammation remains to be clarified. The present study used network pharmacology and screening in a lipopolysaccharide (LPS) model to demonstrate that neferine suppresses the production of inducible nitric oxide synthase, interleukin‑6 and tumor necrosis factor α in LPS‑treated BV‑2 cells. The working concentration of neferine did not exert cytotoxic effects on BV‑2 cells. Mechanistically, neferine attenuated inflammation by inhibiting the phosphorylation and nuclear translocation of the NF‑κB p65 subunit. In vivo, neferine protected mice from the inflammatory response in the substantia nigra and inhibited the development of nervous disorders in the 1‑methyl‑4‑phenyl‑1,2,3,6‑tetrahydropyridine‑induced PD model. The present study demonstrated that neferine inhibited LPS‑mediated activation of microglia by inhibiting NF‑κB signaling. These findings may provide a new reference for the prevention and future treatment of PD.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

DiSabato DJ, Quan N and Godbout JP: Neuroinflammation: The devil is in the details. J Neurochem. 139 (Suppl 2):S136–S153. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Konsman JP: Cytokines in the brain and neuroinflammation: We didn't starve the fire! Pharmaceuticals (Basel). 15:1402022. View Article : Google Scholar : PubMed/NCBI

3 

Yong HYF, Rawji KS, Ghorbani S, Xue M and Yong VW: The benefits of neuroinflammation for the repair of the injured central nervous system. Cell Mol Immunol. 16:540–546. 2019. View Article : Google Scholar : PubMed/NCBI

4 

Leng F and Edison P: Neuroinflammation and microglial activation in Alzheimer disease: Where do we go from here? Nat Rev Neurol. 17:157–172. 2021. View Article : Google Scholar : PubMed/NCBI

5 

Chakraborty B, Mukerjee N, Maitra S, Zehravi M, Mukherjee D, Ghosh A, Massoud EES and Rahman MH: Therapeutic potential of different natural products for the treatment of Alzheimer's disease. Oxid Med Cell Longev. 2022:68738742022. View Article : Google Scholar : PubMed/NCBI

6 

Glass CK, Saijo K, Winner B, Marchetto MC and Fred H: Mechanisms underlying inflammation in neurodegeneration. Cell. 140:918–934. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Kwon HS and Koh SH: Neuroinflammation in neurodegenerative disorders: The roles of microglia and astrocytes. Transl Neurodegener. 9:422020. View Article : Google Scholar : PubMed/NCBI

8 

Fiebich BL, Batista CRA, Saliba SW, Yousif NM and de Oliveira ACP: Role of microglia TLRs in neurodegeneration. Front Cell Neurosci. 12:3292018. View Article : Google Scholar : PubMed/NCBI

9 

Xu L, He D and Bai Y: Microglia-mediated inflammation and neurodegenerative disease. Mol Neurobiol. 53:6709–6715. 2016. View Article : Google Scholar : PubMed/NCBI

10 

Deng M, Yan W, Gu Z, Li Y, Chen L and He B: Anti-neuroinflammatory potential of natural products in the treatment of Alzheimer's disease. Molecules. 28:14862023. View Article : Google Scholar : PubMed/NCBI

11 

Guzman-Martinez L, Maccioni RB, Andrade V, Navarrete LP, Pastor MG and Ramos-Escobar N: Neuroinflammation as a common feature of neurodegenerative disorders. Front Pharmacol. 10:10082019. View Article : Google Scholar : PubMed/NCBI

12 

Terstappen GC, Meyer AH, Bell RD and Zhang W: Strategies for delivering therapeutics across the blood-brain barrier. Nat Rev Drug Discov. 20:362–383. 2021. View Article : Google Scholar : PubMed/NCBI

13 

Lu F, Wang D, Li RL, He LY, Ai L and Wu CJ: Current strategies and technologies for finding drug targets of active components from traditional Chinese medicine. Front Biosci (Landmark Ed). 26:572–589. 2021. View Article : Google Scholar : PubMed/NCBI

14 

Pan T, Cai B, Wang K, Wang S, Zhou S, Yu X, Xu B and Chen L: Neferine enhances insulin sensitivity in insulin resistant rats. J Ethnopharmacol. 124:98–102. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Xu L, Zhang X, Li Y, Lu S, Lu S, Li J, Wang Y, Tian X, Wei JJ, Shao C and Liu Z: Neferine induces autophagy of human ovarian cancer cells via p38 MAPK/JNK activation. Tumor Biol. 37:8721–8729. 2016. View Article : Google Scholar : PubMed/NCBI

16 

Jun MY, Karki R, Paudel KR, Sharma BR, Adhikari D and Kim DW: Alkaloid rich fraction from Nelumbo nucifera targets VSMC proliferation and migration to suppress restenosis in balloon-injured rat carotid artery. Atherosclerosis. 248:179–189. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Wang Y, Wang S, Wang R, Li S and Yuan Y: Neferine exerts antioxidant and anti-inflammatory effects on carbon tetrachloride-induced liver fibrosis by inhibiting the MAPK and NF-κB/IκBα pathways. Evid Based Complement Alternat Med. 2021:41360192021.PubMed/NCBI

18 

Li H, Gao L, Min J, Yang Y and Zhang R: Neferine suppresses autophagy-induced inflammation, oxidative stress and adipocyte differentiation in Graves' orbitopathy. J Cell Mol Med. 25:1949–1957. 2021. View Article : Google Scholar : PubMed/NCBI

19 

Yin S, Ran Q, Yang J, Zhao Y and Li C: Nootropic effect of neferine on aluminium chloride-induced Alzheimer's disease in experimental models. J Biochem Mol Toxicol. 34:e224292020. View Article : Google Scholar : PubMed/NCBI

20 

Lin TY, Hung CY, Chiu KM, Lee MY, Lu CW and Wang SJ: Neferine, an alkaloid from lotus seed embryos, exerts antiseizure and neuroprotective effects in a kainic acid-induced seizure model in rats. Int J Mol Sci. 23:41302022. View Article : Google Scholar : PubMed/NCBI

21 

Wang SH, He H, Chen L, Zhang W, Zhang XJ and Chen JZ: Protective effects of salidroside in the MPTP/MPP(+)-induced model of Parkinson's disease through ROS-NO-related mitochondrion pathway. Mol Neurobiol. 51:718–728. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

23 

Zhong Z, Chen W, Gao H, Che N, Xu M, Yang L, Zhang Y and Ye M: Fecal microbiota transplantation exerts a protective role in MPTP-induced parkinson's disease via the TLR4/PI3K/AKT/NF-κB pathway stimulated by α-synuclein. Neurochem Res. 46:3050–3058. 2021. View Article : Google Scholar : PubMed/NCBI

24 

Hiramatsu G, Uta D, Mihara K, Andoh T and Kume T: Inhibitory effect of panaxytriol on BV-2 microglial cell activation. J Pharmacol Sci. 145:273–278. 2021. View Article : Google Scholar : PubMed/NCBI

25 

Shin HM, Kim MH, Kim BH, Jung SH, Kim YS, Park HJ, Hong JT, Min KR and Kim Y: Inhibitory action of novel aromatic diamine compound on lipopolysaccharide-induced nuclear translocation of NF-kappaB without affecting IkappaB degradation. FEBS Lett. 571:50–54. 2004. View Article : Google Scholar : PubMed/NCBI

26 

Verri M, Pastoris O, Dossena M, Aquilani R, Guerriero F, Cuzzoni G, Venturini L, Ricevuti G and Bongiorno AI: Mitochondrial alterations, oxidative stress and neuroinflammation in Alzheimer's disease. Int J Immunopathol Pharmacol. 25:345–353. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Lull ME and Block ML: Microglial activation and chronic neurodegeneration. Neurotherapeutics. 7:354–365. 2010. View Article : Google Scholar : PubMed/NCBI

28 

Chiu KM, Hung YL, Wang SJ, Tsai YJ, Wu NL, Liang CW, Chang DC and Hung CF: Anti-allergic and anti-inflammatory effects of neferine on RBL-2H3 cells. Int J Mol Sci. 22:109942021. View Article : Google Scholar : PubMed/NCBI

29 

Zhu JJ, Yu BY, Huang XK, He MZ, Chen BW, Chen TT, Fang HY, Chen SQ, Fu XQ, Li PJ, et al: Neferine protects against hypoxic-ischemic brain damage in neonatal rats by suppressing NLRP3-mediated inflammasome activation. Oxid Med Cell Longev. 2021:66549542021. View Article : Google Scholar : PubMed/NCBI

30 

Wu XF, Li C, Yang G, Wang YZ, Peng Y, Zhu DD, Sui AR, Wu Q, Li QF, Wang B, et al: Scorpion venom heat-resistant peptide attenuates microglia activation and neuroinflammation. Front Pharmacol. 12:7047152021. View Article : Google Scholar : PubMed/NCBI

31 

Yu J, Zhu H, Taheri S, Mondy W, Bonilha L, Magwood GS, Lackland D, Adams RJ and Kindy MS: Serum amyloid A-mediated inflammasome activation of microglial cells in cerebral ischemia. J Neurosci. 39:9465–9476. 2019. View Article : Google Scholar : PubMed/NCBI

32 

Li D, Xu J, Qin Y, Cai N, Cheng Y and Wang H: Roflupram, a novel phosphodiesterase 4 inhibitor, inhibits lipopolysaccharide-induced neuroinflammatory responses through activation of the AMPK/Sirt1 pathway. Int Immunopharmacol. 90:1071762021. View Article : Google Scholar : PubMed/NCBI

33 

Xie J, Chen MH, Ying CP and Chen MY: Neferine induces p38 MAPK/JNK1/2 activation to modulate melanoma proliferation, apoptosis, and oxidative stress. Ann Transl Med. 8:16432020. View Article : Google Scholar : PubMed/NCBI

34 

Chen S, Chu B, Chen Y, Cheng X, Guo D, Chen L, Wang J, Li Z, Hong Z and Hong D: Neferine suppresses osteoclast differentiation through suppressing NF-κB signal pathway but not MAPKs and promote osteogenesis. J Cell Physiol. 234:22960–22971. 2019. View Article : Google Scholar : PubMed/NCBI

35 

Ni B, Huang X, Xi Y, Mao Z, Chu X, Zhang R, Ma X and You H: Neferine inhibits expression of inflammatory mediators and matrix degrading enzymes in IL-1β-treated rat chondrocytes via suppressing MAPK and NF-κB signaling pathways. Inflammation. 43:1209–1221. 2020. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Li T, Zhai YX, Zheng T and Xu B: Neferine exerts anti‑inflammatory activity in BV‑2 microglial cells and protects mice with MPTP‑induced Parkinson's disease by inhibiting NF‑κB activation. Mol Med Rep 28: 235, 2023.
APA
Li, T., Zhai, Y.X., Zheng, T., & Xu, B. (2023). Neferine exerts anti‑inflammatory activity in BV‑2 microglial cells and protects mice with MPTP‑induced Parkinson's disease by inhibiting NF‑κB activation. Molecular Medicine Reports, 28, 235. https://doi.org/10.3892/mmr.2023.13122
MLA
Li, T., Zhai, Y. X., Zheng, T., Xu, B."Neferine exerts anti‑inflammatory activity in BV‑2 microglial cells and protects mice with MPTP‑induced Parkinson's disease by inhibiting NF‑κB activation". Molecular Medicine Reports 28.6 (2023): 235.
Chicago
Li, T., Zhai, Y. X., Zheng, T., Xu, B."Neferine exerts anti‑inflammatory activity in BV‑2 microglial cells and protects mice with MPTP‑induced Parkinson's disease by inhibiting NF‑κB activation". Molecular Medicine Reports 28, no. 6 (2023): 235. https://doi.org/10.3892/mmr.2023.13122
Copy and paste a formatted citation
x
Spandidos Publications style
Li T, Zhai YX, Zheng T and Xu B: Neferine exerts anti‑inflammatory activity in BV‑2 microglial cells and protects mice with MPTP‑induced Parkinson's disease by inhibiting NF‑κB activation. Mol Med Rep 28: 235, 2023.
APA
Li, T., Zhai, Y.X., Zheng, T., & Xu, B. (2023). Neferine exerts anti‑inflammatory activity in BV‑2 microglial cells and protects mice with MPTP‑induced Parkinson's disease by inhibiting NF‑κB activation. Molecular Medicine Reports, 28, 235. https://doi.org/10.3892/mmr.2023.13122
MLA
Li, T., Zhai, Y. X., Zheng, T., Xu, B."Neferine exerts anti‑inflammatory activity in BV‑2 microglial cells and protects mice with MPTP‑induced Parkinson's disease by inhibiting NF‑κB activation". Molecular Medicine Reports 28.6 (2023): 235.
Chicago
Li, T., Zhai, Y. X., Zheng, T., Xu, B."Neferine exerts anti‑inflammatory activity in BV‑2 microglial cells and protects mice with MPTP‑induced Parkinson's disease by inhibiting NF‑κB activation". Molecular Medicine Reports 28, no. 6 (2023): 235. https://doi.org/10.3892/mmr.2023.13122
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team