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Overexpression of salusin‑β downregulates adipoR1 expression to prevent fatty acid oxidation in HepG2 cells

  • Authors:
    • Aohong Xu
    • Lei Wang
    • Min Luo
    • Huan Zhang
    • Meiwei Ning
    • Jintong Pan
    • Xiuqun Duan
    • Yuxue Wang
    • Xiang Liu
  • View Affiliations / Copyright

    Affiliations: Department of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan, Hubei 430065, P.R. China, Jiamusi College, Heilongjiang University of Chinese Medicine, Jiamusi, Heilongjiang 154007, P.R. China, Clinical Laboratory, Ezhou Central Hospital, Ezhou, Hubei 436000, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 18
    |
    Published online on: December 7, 2023
       https://doi.org/10.3892/mmr.2023.13141
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Abstract

Salusin‑β and adiponectin receptor 1 (adipoR1) serve important roles in the development of certain cardiovascular diseases and lipid metabolism. However, to the best of our knowledge, the relationship between salusin‑β and adipoR1, and their underlying mechanisms of action, currently remain unclear. In the present study, lentiviral vectors designed to overexpress salusin‑β or knock down salusin‑β expression were used in 293T and HepG2 cells. Semi‑quantitative PCR was performed to investigate the relationship between salusin‑β and adipoR1 mRNA expression in 293T cells. Western blotting was used to assess the protein expression levels of adipoR1, adenosine monophosphate‑activated protein kinase (AMPK), acetyl‑CoA carboxylase (ACC) and carnitine palmitoyl transferase 1A (CPT‑1A) in transfected HepG2 cells. Simultaneously, HepG2 cells were treated with an adipoR1 inhibitor (thapsigargin) or agonist (AdipoRon) and the resultant changes in the expression levels of the aforementioned proteins were observed. Oil Red O staining and measurements of cellular triglyceride levels were performed to assess the extent of lipid accumulation in HepG2 cells. The results demonstrated that salusin‑β overexpression downregulated adipoR1 expression and inhibited the phosphorylation of AMPK and ACC, which led to decreased CPT‑1A protein expression. By contrast, salusin‑β knockdown increased adipoR1 expression and promoted the phosphorylation of AMPK and ACC, which conversely enhanced CPT‑1A protein expression. Treatment with adipoR1 agonist, AdipoRon, reversed the effects of salusin‑β overexpression. In addition, salusin‑β overexpression enhanced intracellular lipid accumulation in HepG2 cells induced by free fatty acid treatment. These findings highlighted the potential regulatory role of salusin‑β in adipoR1‑mediated signaling pathways. To conclude, the present study provided insights into the regulation of fatty acid metabolism by the liver. In particular, salusin‑β may serve as a potential target for the therapeutic intervention of metabolic disorders of lipids.
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Copy and paste a formatted citation
Spandidos Publications style
Xu A, Wang L, Luo M, Zhang H, Ning M, Pan J, Duan X, Wang Y and Liu X: Overexpression of salusin‑β downregulates adipoR1 expression to prevent fatty acid oxidation in HepG2 cells. Mol Med Rep 29: 18, 2024.
APA
Xu, A., Wang, L., Luo, M., Zhang, H., Ning, M., Pan, J. ... Liu, X. (2024). Overexpression of salusin‑β downregulates adipoR1 expression to prevent fatty acid oxidation in HepG2 cells. Molecular Medicine Reports, 29, 18. https://doi.org/10.3892/mmr.2023.13141
MLA
Xu, A., Wang, L., Luo, M., Zhang, H., Ning, M., Pan, J., Duan, X., Wang, Y., Liu, X."Overexpression of salusin‑β downregulates adipoR1 expression to prevent fatty acid oxidation in HepG2 cells". Molecular Medicine Reports 29.2 (2024): 18.
Chicago
Xu, A., Wang, L., Luo, M., Zhang, H., Ning, M., Pan, J., Duan, X., Wang, Y., Liu, X."Overexpression of salusin‑β downregulates adipoR1 expression to prevent fatty acid oxidation in HepG2 cells". Molecular Medicine Reports 29, no. 2 (2024): 18. https://doi.org/10.3892/mmr.2023.13141
Copy and paste a formatted citation
x
Spandidos Publications style
Xu A, Wang L, Luo M, Zhang H, Ning M, Pan J, Duan X, Wang Y and Liu X: Overexpression of salusin‑β downregulates adipoR1 expression to prevent fatty acid oxidation in HepG2 cells. Mol Med Rep 29: 18, 2024.
APA
Xu, A., Wang, L., Luo, M., Zhang, H., Ning, M., Pan, J. ... Liu, X. (2024). Overexpression of salusin‑β downregulates adipoR1 expression to prevent fatty acid oxidation in HepG2 cells. Molecular Medicine Reports, 29, 18. https://doi.org/10.3892/mmr.2023.13141
MLA
Xu, A., Wang, L., Luo, M., Zhang, H., Ning, M., Pan, J., Duan, X., Wang, Y., Liu, X."Overexpression of salusin‑β downregulates adipoR1 expression to prevent fatty acid oxidation in HepG2 cells". Molecular Medicine Reports 29.2 (2024): 18.
Chicago
Xu, A., Wang, L., Luo, M., Zhang, H., Ning, M., Pan, J., Duan, X., Wang, Y., Liu, X."Overexpression of salusin‑β downregulates adipoR1 expression to prevent fatty acid oxidation in HepG2 cells". Molecular Medicine Reports 29, no. 2 (2024): 18. https://doi.org/10.3892/mmr.2023.13141
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