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SFRP2 suppresses trophoblast cell migration by inhibiting the Wnt/β‑catenin pathway

  • Authors:
    • Ruihong Lan
    • Yihong Yu
    • Jie Song
    • Mengdi Xue
    • Humin Gong
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, Hainan 570311, P.R. China, School of Clinical Medicine, Hainan Medical University, Haikou, Hainan 571199, P.R. China
    Copyright: © Lan et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].
  • Article Number: 66
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    Published online on: February 28, 2024
       https://doi.org/10.3892/mmr.2024.13190
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Abstract

The present study investigates the role of Secreted Frizzled‑Related Protein 2 (SFRP2) in trophoblast cells, a key factor in preeclampsia (PE) progression. Elevated levels of Secreted Frizzled‑Related Protein 1/3/4/5 (SFRP1/3/4/5) are associated with PE, but the role of SFRP2 is unclear. We analyzed SFRP2 expression in PE placental tissue using the GSE10588 dataset and overexpressed SFRP2 in JEG‑3 cells via lentiviral transfection. The viability, migration, apoptosis, and proliferation of SFRP2‑overexpressing JEG‑3 cells were assessed using Cell Counting Kit‑8, Transwell assays, flow cytometry, and EdU staining. Additionally, we evaluated the impact of SFRP2 overexpression on key proteins in the Wnt/β‑catenin pathway and apoptosis markers (Bax, cleaved‑caspase 3, BCL‑2, MMP9, E‑cadherin, Wnt3a, Axin2, CyclinD1, c‑Myc, p‑β‑catenin, β‑catenin, phosphorylated Glycogen Synthase Kinase 3 beta (p‑GSK3β), and GSK3β) through western blotting. Results showed high SFRP2 mRNA and protein expression in PE placenta and JEG‑3 cells post‑transfection. SFRP2 overexpression significantly reduced JEG‑3 cell viability, proliferation, and migration, while increasing apoptosis. It also altered expression levels of Wnt pathway proteins, suggesting SFRP2's potential as a therapeutic target for PE by inhibiting trophoblast cell migration through the Wnt/β‑catenin signaling cascade.
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Copy and paste a formatted citation
Spandidos Publications style
Lan R, Yu Y, Song J, Xue M and Gong H: SFRP2 suppresses trophoblast cell migration by inhibiting the Wnt/β‑catenin pathway. Mol Med Rep 29: 66, 2024.
APA
Lan, R., Yu, Y., Song, J., Xue, M., & Gong, H. (2024). SFRP2 suppresses trophoblast cell migration by inhibiting the Wnt/β‑catenin pathway. Molecular Medicine Reports, 29, 66. https://doi.org/10.3892/mmr.2024.13190
MLA
Lan, R., Yu, Y., Song, J., Xue, M., Gong, H."SFRP2 suppresses trophoblast cell migration by inhibiting the Wnt/β‑catenin pathway". Molecular Medicine Reports 29.4 (2024): 66.
Chicago
Lan, R., Yu, Y., Song, J., Xue, M., Gong, H."SFRP2 suppresses trophoblast cell migration by inhibiting the Wnt/β‑catenin pathway". Molecular Medicine Reports 29, no. 4 (2024): 66. https://doi.org/10.3892/mmr.2024.13190
Copy and paste a formatted citation
x
Spandidos Publications style
Lan R, Yu Y, Song J, Xue M and Gong H: SFRP2 suppresses trophoblast cell migration by inhibiting the Wnt/β‑catenin pathway. Mol Med Rep 29: 66, 2024.
APA
Lan, R., Yu, Y., Song, J., Xue, M., & Gong, H. (2024). SFRP2 suppresses trophoblast cell migration by inhibiting the Wnt/β‑catenin pathway. Molecular Medicine Reports, 29, 66. https://doi.org/10.3892/mmr.2024.13190
MLA
Lan, R., Yu, Y., Song, J., Xue, M., Gong, H."SFRP2 suppresses trophoblast cell migration by inhibiting the Wnt/β‑catenin pathway". Molecular Medicine Reports 29.4 (2024): 66.
Chicago
Lan, R., Yu, Y., Song, J., Xue, M., Gong, H."SFRP2 suppresses trophoblast cell migration by inhibiting the Wnt/β‑catenin pathway". Molecular Medicine Reports 29, no. 4 (2024): 66. https://doi.org/10.3892/mmr.2024.13190
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