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MicroRNA‑155‑5p inhibits trophoblast cell proliferation and invasion by disrupting centrosomal function

  • Authors:
    • Yung-Chieh Tsai
    • Tian-Ni Kuo
    • Ruei-Ci Lin
    • Hui-Ling Tsai
    • Yu-Ying Chao
    • Pei-Rong Lee
    • Ping-Jui Su
    • Chia-Yih Wang
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Chi‑Mei Medical Center, Tainan 710, Taiwan, R.O.C., Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan, R.O.C., Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan, R.O.C., Division of General Surgery, Department of Surgery, National Cheng Kung University Hospital, Tainan 704, Taiwan, R.O.C.
    Copyright: © Tsai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 85
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    Published online on: March 22, 2024
       https://doi.org/10.3892/mmr.2024.13209
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Abstract

Recurrent miscarriage is used to refer to more than three pregnancy failures before 20 weeks of gestation. Defective trophoblast cell growth and invasion are frequently observed in recurrent miscarriage. Several microRNAs (miRs), including miR‑155‑5p, are aberrantly upregulated in recurrent miscarriage; however, the underlying molecular mechanisms remain unclear. The centrosome orchestrates microtubule networks and coordinates cell cycle progression. In addition, it is a base for primary cilia, which are antenna‑like organelles that coordinate signaling during development and growth. Thus, deficiencies in centrosomal functions can lead to several disease, such as breast cancer and microcephaly. In the present study, the signaling cascades were analyzed by western blotting, and the centrosome and primary cilia were observed and analyzed by immunofluorescence staining. The results showed that overexpression of miR‑155‑5p induced centrosome amplification and blocked primary cilia formation in trophoblast cells. Notably, centrosome amplification inhibited trophoblast cell growth by upregulating apoptotic cleaved‑caspase 3 and cleaved‑poly (ADP‑ribose) polymerase in miR‑155‑5p‑overexpressing trophoblast cells. In addition, overexpression of miR‑155‑5p inhibited primary cilia formation, thereby inhibiting epithelial‑mesenchymal transition and trophoblast cell invasion. All phenotypes could be rescued when cells were co‑transfected with the miR‑155‑5p inhibitor, thus supporting the role of miR‑155‑5p in centrosomal functions. It was also found that miR‑155‑5p activated autophagy, whereas disruption of autophagy via the depletion of autophagy‑related 16‑like 1 alleviated miR‑155‑5p‑induced apoptosis and restored trophoblast cell invasion. In conclusion, the present study indicated a novel role of miR‑55‑5p in mediating centrosomal function in recurrent miscarriage.
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Copy and paste a formatted citation
Spandidos Publications style
Tsai Y, Kuo T, Lin R, Tsai H, Chao Y, Lee P, Su P and Wang C: MicroRNA‑155‑5p inhibits trophoblast cell proliferation and invasion by disrupting centrosomal function. Mol Med Rep 29: 85, 2024.
APA
Tsai, Y., Kuo, T., Lin, R., Tsai, H., Chao, Y., Lee, P. ... Wang, C. (2024). MicroRNA‑155‑5p inhibits trophoblast cell proliferation and invasion by disrupting centrosomal function. Molecular Medicine Reports, 29, 85. https://doi.org/10.3892/mmr.2024.13209
MLA
Tsai, Y., Kuo, T., Lin, R., Tsai, H., Chao, Y., Lee, P., Su, P., Wang, C."MicroRNA‑155‑5p inhibits trophoblast cell proliferation and invasion by disrupting centrosomal function". Molecular Medicine Reports 29.5 (2024): 85.
Chicago
Tsai, Y., Kuo, T., Lin, R., Tsai, H., Chao, Y., Lee, P., Su, P., Wang, C."MicroRNA‑155‑5p inhibits trophoblast cell proliferation and invasion by disrupting centrosomal function". Molecular Medicine Reports 29, no. 5 (2024): 85. https://doi.org/10.3892/mmr.2024.13209
Copy and paste a formatted citation
x
Spandidos Publications style
Tsai Y, Kuo T, Lin R, Tsai H, Chao Y, Lee P, Su P and Wang C: MicroRNA‑155‑5p inhibits trophoblast cell proliferation and invasion by disrupting centrosomal function. Mol Med Rep 29: 85, 2024.
APA
Tsai, Y., Kuo, T., Lin, R., Tsai, H., Chao, Y., Lee, P. ... Wang, C. (2024). MicroRNA‑155‑5p inhibits trophoblast cell proliferation and invasion by disrupting centrosomal function. Molecular Medicine Reports, 29, 85. https://doi.org/10.3892/mmr.2024.13209
MLA
Tsai, Y., Kuo, T., Lin, R., Tsai, H., Chao, Y., Lee, P., Su, P., Wang, C."MicroRNA‑155‑5p inhibits trophoblast cell proliferation and invasion by disrupting centrosomal function". Molecular Medicine Reports 29.5 (2024): 85.
Chicago
Tsai, Y., Kuo, T., Lin, R., Tsai, H., Chao, Y., Lee, P., Su, P., Wang, C."MicroRNA‑155‑5p inhibits trophoblast cell proliferation and invasion by disrupting centrosomal function". Molecular Medicine Reports 29, no. 5 (2024): 85. https://doi.org/10.3892/mmr.2024.13209
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