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Induction of the human CDC45 gene promoter activity by natural compound trans‑resveratrol

  • Authors:
    • Jun Arakawa
    • Hikaru Kondoh
    • Tokiyoshi Matsushita
    • Yoko Ogino
    • Masashi Asai
    • Sei-Ichi Tanuma
    • Fumiaki Uchiumi
  • View Affiliations / Copyright

    Affiliations: Department of Gene Regulation, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Chiba 278‑8510, Japan, Genomic Medicinal Science, Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba 278‑8510, Japan
    Copyright: © Arakawa et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 92
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    Published online on: April 4, 2024
       https://doi.org/10.3892/mmr.2024.13216
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Abstract

GGAA motifs in the human TP53 and HELB gene promoters play a part in responding to trans‑resveratrol (Rsv) in HeLa S3 cells. This sequence is also present in the 5'‑upstream region of the human CDC45 gene, which encodes a component of CMG DNA helicase protein complex. The cells were treated with Rsv (20 µM), then transcripts and the translated protein were analyzed by quantitative RT‑PCR and western blotting, respectively. The results showed that the CDC45 gene and protein expression levels were induced after the treatment. To examine whether they were due to the activation of transcription, a 5'‑upstream 556‑bp of the CDC45 gene was cloned and inserted into a multi‑cloning site of the Luciferase (Luc) expression vector. In the present study, various deletion/point mutation‑introduced Luc expression plasmids were constructed and they were used for the transient transfection assay. The results showed that the GGAA motif, which is included in a putative RELB protein recognizing sequence, plays a part in the promoter activity with response to Rsv in HeLa S3 cells.
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Copy and paste a formatted citation
Spandidos Publications style
Arakawa J, Kondoh H, Matsushita T, Ogino Y, Asai M, Tanuma S and Uchiumi F: Induction of the human <em>CDC45</em> gene promoter activity by natural compound <em>trans</em>‑resveratrol. Mol Med Rep 29: 92, 2024.
APA
Arakawa, J., Kondoh, H., Matsushita, T., Ogino, Y., Asai, M., Tanuma, S., & Uchiumi, F. (2024). Induction of the human <em>CDC45</em> gene promoter activity by natural compound <em>trans</em>‑resveratrol. Molecular Medicine Reports, 29, 92. https://doi.org/10.3892/mmr.2024.13216
MLA
Arakawa, J., Kondoh, H., Matsushita, T., Ogino, Y., Asai, M., Tanuma, S., Uchiumi, F."Induction of the human <em>CDC45</em> gene promoter activity by natural compound <em>trans</em>‑resveratrol". Molecular Medicine Reports 29.6 (2024): 92.
Chicago
Arakawa, J., Kondoh, H., Matsushita, T., Ogino, Y., Asai, M., Tanuma, S., Uchiumi, F."Induction of the human <em>CDC45</em> gene promoter activity by natural compound <em>trans</em>‑resveratrol". Molecular Medicine Reports 29, no. 6 (2024): 92. https://doi.org/10.3892/mmr.2024.13216
Copy and paste a formatted citation
x
Spandidos Publications style
Arakawa J, Kondoh H, Matsushita T, Ogino Y, Asai M, Tanuma S and Uchiumi F: Induction of the human <em>CDC45</em> gene promoter activity by natural compound <em>trans</em>‑resveratrol. Mol Med Rep 29: 92, 2024.
APA
Arakawa, J., Kondoh, H., Matsushita, T., Ogino, Y., Asai, M., Tanuma, S., & Uchiumi, F. (2024). Induction of the human <em>CDC45</em> gene promoter activity by natural compound <em>trans</em>‑resveratrol. Molecular Medicine Reports, 29, 92. https://doi.org/10.3892/mmr.2024.13216
MLA
Arakawa, J., Kondoh, H., Matsushita, T., Ogino, Y., Asai, M., Tanuma, S., Uchiumi, F."Induction of the human <em>CDC45</em> gene promoter activity by natural compound <em>trans</em>‑resveratrol". Molecular Medicine Reports 29.6 (2024): 92.
Chicago
Arakawa, J., Kondoh, H., Matsushita, T., Ogino, Y., Asai, M., Tanuma, S., Uchiumi, F."Induction of the human <em>CDC45</em> gene promoter activity by natural compound <em>trans</em>‑resveratrol". Molecular Medicine Reports 29, no. 6 (2024): 92. https://doi.org/10.3892/mmr.2024.13216
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