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Inhibitory effect of recombinant tyrosine‑sulfated madanin‑1, a thrombin inhibitor, on the behavior of MDA‑MB‑231 and SKOV3 cells in vitro

  • Authors:
    • Guk Heui Jo
    • Sun Ah Jung
    • Tae Hoon Roh
    • Jin Sook Yoon
    • Joon H. Lee
  • View Affiliations / Copyright

    Affiliations: Myung‑Gok Eye Research Institute, Kim's Eye Hospital, Konyang University College of Medicine, Seoul 07301, Republic of Korea, Department of Medicine, Yonsei University College of Medicine, Seoul 03722, Republic of Korea, Department of Ophthalmology, Institute of Vision Research, Severance Hospital, Yonsei University College of Medicine, Seoul 03722, Republic of Korea
    Copyright: © Jo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 114
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    Published online on: May 8, 2024
       https://doi.org/10.3892/mmr.2024.13238
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Abstract

Thrombin, which plays a crucial role in hemostasis, is also implicated in cancer progression. In the present study, the effects of the thrombin‑targeting recombinant tyrosine‑sulfated madanin‑1 on cancer cell behavior and signaling pathways compared with madanin‑1 wild‑type (WT) were investigated. Recombinant madanin‑1 2 sulfation (madanin‑1 2S) and madanin‑1 WT proteins were generated using Escherichia coli. SKOV3 and MDA‑MB‑231 cells were treated with purified recombinant proteins with or without thrombin stimulation. Migration and invasion of cells were analyzed by wound healing assay and Transwell assay, respectively. Thrombin markedly increased cell migration and invasion in both SKOV3 and MDA‑MB‑231 cells, which were significantly suppressed by madanin‑1 2S (P<0.05). Madanin‑1 2S also significantly suppressed thrombin‑induced expression of phosphorylated (p)‑Akt and p‑extracellular signal‑regulated kinase in both cell lines (P<0.05), whereas madanin‑1 WT had no effect on the expression levels of these proteins in MDA‑MB‑231 cells. Furthermore, madanin‑1 2S significantly reversed the effects of thrombin on E‑cadherin, N‑cadherin and vimentin expression in MDA‑MB‑231 cells (P<0.05), whereas madanin‑1 WT did not show any effect. In conclusion, madanin‑1 2S suppressed the migration and invasion of cancer cells more effectively than madanin‑1 WT. It is hypothesized that inhibiting thrombin via the sulfated form of madanin‑1 may be a potential candidate for enhanced cancer therapy; however, further in vivo validation is required.
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View References

1 

Larsen JB and Hvas AM: Thrombin: A pivotal player in hemostasis and beyond. Semin Thromb Hemost. 47:759–774. 2021. View Article : Google Scholar : PubMed/NCBI

2 

Chen LB and Buchanan JM: Mitogenic activity of blood components. I. Thrombin and prothrombin. Proc Natl Acad Sci USA. 72:131–135. 1975. View Article : Google Scholar : PubMed/NCBI

3 

Maruyama I, Shigeta K, Miyahara H, Nakajima T, Shin H, Ide S and Kitajima I: Thrombin activates NF-kappa B through thrombin receptor and results in proliferation of vascular smooth muscle cells: Role of thrombin in atherosclerosis and restenosis. Ann N Y Acad Sci. 811:429–436. 1997. View Article : Google Scholar : PubMed/NCBI

4 

Zhou S, Xiao W, Pan X, Zhu M, Yang Z, Zhang F and Zheng C: Thrombin promotes proliferation of human lung fibroblasts via protease activated receptor-1-dependent and NF-κB-independent pathways. Cell Biol Int. 38:747–756. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Szaba FM and Smiley ST: Roles for thrombin and fibrin(ogen) in cytokine/chemokine production and macrophage adhesion in vivo. Blood. 99:1053–1059. 2002. View Article : Google Scholar : PubMed/NCBI

6 

Chiang HS, Yang RS and Huang TF: Thrombin enhances the adhesion and migration of human colon adenocarcinoma cells via increased beta 3-integrin expression on the tumour cell surface and their inhibition by the snake venom peptide, rhodostomin. Br J Cancer. 73:902–908. 1996. View Article : Google Scholar : PubMed/NCBI

7 

Nierodzik ML and Karpatkin S: Thrombin induces tumor growth, metastasis, and angiogenesis: Evidence for a thrombin-regulated dormant tumor phenotype. Cancer Cell. 10:355–362. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Franchini M and Mannucci PM: Thrombin and cancer: From molecular basis to therapeutic implications. Semin Thromb Hemost. 38:95–101. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Cantrell R and Palumbo JS: The thrombin-inflammation axis in cancer progression. Thromb Res. 191 (Suppl 1):S117–S122. 2020. View Article : Google Scholar : PubMed/NCBI

10 

Covic L and Kuliopulos A: Protease-activated receptor 1 as therapeutic target in breast, lung, and ovarian cancer: Pepducin approach. Int J Mol Sci. 19:22372018. View Article : Google Scholar : PubMed/NCBI

11 

Liu X, Yu J, Song S, Yue X and Li Q: Protease-activated receptor-1 (PAR-1): A promising molecular target for cancer. Oncotarget. 8:107334–107345. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Jacobs JW, Cupp EW, Sardana M and Friedman PA: Isolation and characterization of a coagulation factor Xa inhibitor from black fly salivary glands. Thromb Haemost. 64:235–238. 1990. View Article : Google Scholar : PubMed/NCBI

13 

Waxman L, Smith DE, Arcuri KE and Vlasuk GP: Tick anticoagulant peptide (TAP) is a novel inhibitor of blood coagulation factor Xa. Science. 248:593–596. 1990. View Article : Google Scholar : PubMed/NCBI

14 

Salzet M: Leech thrombin inhibitors. Curr Pharm Des. 8:493–503. 2002. View Article : Google Scholar : PubMed/NCBI

15 

Figueiredo AC, de Sanctis D and Pereira PJ: The tick-derived anticoagulant madanin is processed by thrombin and factor Xa. PLoS One. 8:e718662013. View Article : Google Scholar : PubMed/NCBI

16 

Iwanaga S, Okada M, Isawa H, Morita A, Yuda M and Chinzei Y: Identification and characterization of novel salivary thrombin inhibitors from the ixodidae tick, Haemaphysalis longicornis. Eur J Biochem. 270:1926–1934. 2003. View Article : Google Scholar : PubMed/NCBI

17 

Thompson RE, Liu X, Ripoll-Rozada J, Alonso-Garcia N, Parker BL, Pereira PJB and Payne RJ: Tyrosine sulfation modulates activity of tick-derived thrombin inhibitors. Nat Chem. 9:909–917. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Khorana AA, Mackman N, Falanga A, Pabinger I, Noble S, Ageno W, Moik F and Lee AYY: Cancer-associated venous thromboembolism. Nat Rev Dis Primers. 8:112022. View Article : Google Scholar : PubMed/NCBI

19 

Asanuma K, Wakabayashi H, Okamoto T, Asanuma Y, Akita N, Yoshikawa T, Hayashi T, Matsumine A, Uchida A and Sudo A: The thrombin inhibitor, argatroban, inhibits breast cancer metastasis to bone. Breast Cancer. 20:241–246. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Alexander ET, Minton AR, Peters MC, van Ryn J and Gilmour SK: Thrombin inhibition and cisplatin block tumor progression in ovarian cancer by alleviating the immunosuppressive microenvironment. Oncotarget. 7:85291–85305. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Chanakira A, Westmark PR, Ong IM and Sheehan JP: Tissue factor-factor VIIa complex triggers protease activated receptor 2-dependent growth factor release and migration in ovarian cancer. Gynecol Oncol. 145:167–175. 2017. View Article : Google Scholar : PubMed/NCBI

22 

Chaffer CL and Weinberg RA: A perspective on cancer cell metastasis. Science. 331:1559–1564. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Radjabi AR, Sawada K, Jagadeeswaran S, Eichbichler A, Kenny HA, Montag A, Bruno K and Lengyel E: Thrombin induces tumor invasion through the induction and association of matrix metalloproteinase-9 and beta1-integrin on the cell surface. J Biol Chem. 283:2822–2834. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Kim J, Lee JW, Kim SI, Choi YJ, Lee WK, Jeong MJ, Cha SH, Lee HJ, Chun W and Kim SS: Thrombin-induced migration and matrix metalloproteinase-9 expression are regulated by MAPK and PI3K pathways in C6 glioma cells. Korean J Physiol Pharmacol. 15:211–216. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Zhong YC, Zhang T, Di W and Li WP: Thrombin promotes epithelial ovarian cancer cell invasion by inducing epithelial-mesenchymal transition. J Gynecol Oncol. 24:265–272. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Justus CR, Leffler N, Ruiz-Echevarria M and Yang LV: In vitro cell migration and invasion assays. J Vis Exp. 510462014.PubMed/NCBI

27 

Liu CC, Cellitti SE, Geierstanger BH and Schultz PG: Efficient expression of tyrosine-sulfated proteins in E. coli using an expanded genetic code. Nat Protoc. 4:1784–1789. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Kirwan CC, Descamps T and Castle J: Circulating tumour cells and hypercoagulability: A lethal relationship in metastatic breast cancer. Clin Transl Oncol. 22:870–877. 2020. View Article : Google Scholar : PubMed/NCBI

29 

Reddel CJ, Tan CW and Chen VM: Thrombin generation and cancer: Contributors and consequences. Cancers (Basel). 11:1002019. View Article : Google Scholar : PubMed/NCBI

30 

Shaker H, Bundred NJ, Landberg G, Pritchard SA, Albadry H, Nicholson SL, Harries LJ, Heah JYE, Castle J and Kirwan CC: Breast cancer stromal clotting activation (tissue factor and thrombin): A pre-invasive phenomena that is prognostic in invasion. Cancer Med. 9:1768–1778. 2020. View Article : Google Scholar : PubMed/NCBI

31 

Ossovskaya VS and Bunnett NW: Protease-activated receptors: Contribution to physiology and disease. Physiol Rev. 84:579–621. 2004. View Article : Google Scholar : PubMed/NCBI

32 

Wojtukiewicz MZ, Hempel D, Sierko E, Tucker SC and Honn KV: Protease-activated receptors (PARs)-biology and role in cancer invasion and metastasis. Cancer Metastasis Rev. 34:775–796. 2015. View Article : Google Scholar : PubMed/NCBI

33 

Alexander ET and Gilmour SK: Immunomodulatory role of thrombin in cancer progression. Mol Carcinog. 61:527–536. 2022. View Article : Google Scholar : PubMed/NCBI

34 

Ohshiro K, Bui-Nguyen TM, Divijendra Natha RS, Schwartz AM, Levine P and Kumar R: Thrombin stimulation of inflammatory breast cancer cells leads to aggressiveness via the EGFR-PAR1-Pak1 pathway. Int J Biol Markers. 27:e305–e313. 2012. View Article : Google Scholar : PubMed/NCBI

35 

Zhao B, Wu M, Hu Z, Wang T, Yu J, Ma Y, Wang Q, Zhang Y, Chen D, Li T, et al: A novel oncotherapy strategy: Direct thrombin inhibitors suppress progression, dissemination and spontaneous metastasis in non-small cell lung cancer. Br J Pharmacol. 179:5056–5073. 2022. View Article : Google Scholar : PubMed/NCBI

36 

Yang YS, Wang CC, Chen BH, Hou YH, Hung KS and Mao YC: Tyrosine sulfation as a protein post-translational modification. Molecules. 20:2138–2164. 2015. View Article : Google Scholar : PubMed/NCBI

37 

Corral-Rodriguez MA, Macedo-Ribeiro S, Pereira PJ and Fuentes-Prior P: Leech-derived thrombin inhibitors: From structures to mechanisms to clinical applications. J Med Chem. 53:3847–3861. 2010. View Article : Google Scholar : PubMed/NCBI

38 

Ouyang YB and Moore KL: Molecular cloning and expression of human and mouse tyrosylprotein sulfotransferase-2 and a tyrosylprotein sulfotransferase homologue in Caenorhabditis elegans. J Biol Chem. 273:24770–24774. 1998. View Article : Google Scholar : PubMed/NCBI

39 

Dongre A and Weinberg RA: New insights into the mechanisms of epithelial-mesenchymal transition and implications for cancer. Nat Rev Mol Cell Biol. 20:69–84. 2019. View Article : Google Scholar : PubMed/NCBI

40 

Huber MA, Kraut N and Beug H: Molecular requirements for epithelial-mesenchymal transition during tumor progression. Curr Opin Cell Biol. 17:548–558. 2005. View Article : Google Scholar : PubMed/NCBI

41 

Zhu X, Wang X, Gong Y and Deng J: E-cadherin on epithelial-mesenchymal transition in thyroid cancer. Cancer Cell Int. 21:6952021. View Article : Google Scholar : PubMed/NCBI

42 

Mrozik KM, Blaschuk OW, Cheong CM, Zannettino ACW and Vandyke K: N-cadherin in cancer metastasis, its emerging role in haematological malignancies and potential as a therapeutic target in cancer. BMC Cancer. 18:9392018. View Article : Google Scholar : PubMed/NCBI

43 

Song Y, Ye M, Zhou J, Wang ZW and Zhu X: Restoring E-cadherin expression by natural compounds for anticancer therapies in genital and urinary cancers. Mol Ther Oncolytics. 14:130–138. 2019. View Article : Google Scholar : PubMed/NCBI

44 

Song Y, Ye M, Zhou J, Wang Z and Zhu X: Targeting E-cadherin expression with small molecules for digestive cancer treatment. Am J Transl Res. 11:3932–3944. 2019.PubMed/NCBI

45 

Otsuki T, Fujimoto D, Hirono Y, Goi T and Yamaguchi A: Thrombin conducts epithelial-mesenchymal transition via protease-activated receptor-1 in human gastric cancer. Int J Oncol. 45:2287–2294. 2014. View Article : Google Scholar : PubMed/NCBI

46 

Cao Z, Liao Q, Su M, Huang K, Jin J and Cao D: AKT and ERK dual inhibitors: The way forward? Cancer Lett. 459:30–40. 2019. View Article : Google Scholar : PubMed/NCBI

47 

Asati V, Mahapatra DK and Bharti SK: PI3K/Akt/mTOR and Ras/Raf/MEK/ERK signaling pathways inhibitors as anticancer agents: Structural and pharmacological perspectives. Eur J Med Chem. 109:314–341. 2016. View Article : Google Scholar : PubMed/NCBI

48 

Karimi Roshan M, Soltani A, Soleimani A, Rezaie Kahkhaie K, Afshari AR and Soukhtanloo M: Role of AKT and mTOR signaling pathways in the induction of epithelial-mesenchymal transition (EMT) process. Biochimie. 165:229–234. 2019. View Article : Google Scholar : PubMed/NCBI

49 

Li Q, Li Z, Luo T and Shi H: Targeting the PI3K/AKT/mTOR and RAF/MEK/ERK pathways for cancer therapy. Mol Biomed. 3:472022. View Article : Google Scholar : PubMed/NCBI

50 

Narayan P, Prowell TM, Gao JJ, Fernandes LL, Li E, Jiang X, Qiu J, Fan J, Song P, Yu J, et al: FDA approval summary: Alpelisib plus fulvestrant for patients with HR-positive, HER2-negative, PIK3CA-mutated, advanced or metastatic breast cancer. Clin Cancer Res. 27:1842–1849. 2021. View Article : Google Scholar : PubMed/NCBI

51 

Yuan Y, Jiang YC, Sun CK and Chen QM: Role of the tumor microenvironment in tumor progression and the clinical applications (review). Oncol Rep. 35:2499–2515. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Jo GH, Jung SA, Roh TH, Yoon JS and Lee JH: Inhibitory effect of recombinant tyrosine‑sulfated madanin‑1, a thrombin inhibitor, on the behavior of MDA‑MB‑231 and SKOV3 cells <em>in vitro</em>. Mol Med Rep 30: 114, 2024.
APA
Jo, G.H., Jung, S.A., Roh, T.H., Yoon, J.S., & Lee, J.H. (2024). Inhibitory effect of recombinant tyrosine‑sulfated madanin‑1, a thrombin inhibitor, on the behavior of MDA‑MB‑231 and SKOV3 cells <em>in vitro</em>. Molecular Medicine Reports, 30, 114. https://doi.org/10.3892/mmr.2024.13238
MLA
Jo, G. H., Jung, S. A., Roh, T. H., Yoon, J. S., Lee, J. H."Inhibitory effect of recombinant tyrosine‑sulfated madanin‑1, a thrombin inhibitor, on the behavior of MDA‑MB‑231 and SKOV3 cells <em>in vitro</em>". Molecular Medicine Reports 30.1 (2024): 114.
Chicago
Jo, G. H., Jung, S. A., Roh, T. H., Yoon, J. S., Lee, J. H."Inhibitory effect of recombinant tyrosine‑sulfated madanin‑1, a thrombin inhibitor, on the behavior of MDA‑MB‑231 and SKOV3 cells <em>in vitro</em>". Molecular Medicine Reports 30, no. 1 (2024): 114. https://doi.org/10.3892/mmr.2024.13238
Copy and paste a formatted citation
x
Spandidos Publications style
Jo GH, Jung SA, Roh TH, Yoon JS and Lee JH: Inhibitory effect of recombinant tyrosine‑sulfated madanin‑1, a thrombin inhibitor, on the behavior of MDA‑MB‑231 and SKOV3 cells <em>in vitro</em>. Mol Med Rep 30: 114, 2024.
APA
Jo, G.H., Jung, S.A., Roh, T.H., Yoon, J.S., & Lee, J.H. (2024). Inhibitory effect of recombinant tyrosine‑sulfated madanin‑1, a thrombin inhibitor, on the behavior of MDA‑MB‑231 and SKOV3 cells <em>in vitro</em>. Molecular Medicine Reports, 30, 114. https://doi.org/10.3892/mmr.2024.13238
MLA
Jo, G. H., Jung, S. A., Roh, T. H., Yoon, J. S., Lee, J. H."Inhibitory effect of recombinant tyrosine‑sulfated madanin‑1, a thrombin inhibitor, on the behavior of MDA‑MB‑231 and SKOV3 cells <em>in vitro</em>". Molecular Medicine Reports 30.1 (2024): 114.
Chicago
Jo, G. H., Jung, S. A., Roh, T. H., Yoon, J. S., Lee, J. H."Inhibitory effect of recombinant tyrosine‑sulfated madanin‑1, a thrombin inhibitor, on the behavior of MDA‑MB‑231 and SKOV3 cells <em>in vitro</em>". Molecular Medicine Reports 30, no. 1 (2024): 114. https://doi.org/10.3892/mmr.2024.13238
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