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Article Open Access

Puerariae lobatae Radix ameliorates chronic kidney disease by reshaping gut microbiota and downregulating Wnt/β‑catenin signaling

  • Authors:
    • Peng Wu
    • Jingwen Xue
    • Zhangrui Zhu
    • Yao Yu
    • Qi Sun
    • Ming Xie
    • Benlin Wang
    • Pengcheng Huang
    • Zhengyuan Feng
    • Jie Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, NMPA Key Laboratory for Research and Evaluation of Drug Metabolism, Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].
  • Article Number: 117
    |
    Published online on: May 14, 2024
       https://doi.org/10.3892/mmr.2024.13241
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Abstract

Gut microbiota dysfunction is a key factor affecting chronic kidney disease (CKD) susceptibility. Puerariae lobatae Radix (PLR), a traditional Chinese medicine and food homologous herb, is known to promote the gut microbiota homeostasis; however, its role in renoprotection remains unknown. The present study aimed to investigate the efficacy and potential mechanism of PLR to alleviate CKD. An 8‑week 2% NaCl‑feeding murine model was applied to induce CKD and evaluate the therapeutic effect of PLR supplementary. After gavage for 8 weeks, The medium and high doses of PLR significantly alleviated CKD‑associated creatinine, urine protein increasement and nephritic histopathological injury. Moreover, PLR protected kidney from fibrosis by reducing inflammatory response and downregulating the canonical Wnt/β‑catenin pathway. Furthermore, PLR rescued the gut microbiota dysbiosis and protected against high salt‑induced gut barrier dysfunction. Enrichment of Akkermansia and Bifidobacterium was found after PLR intervention, the relative abundances of which were in positive correlation with normal maintenance of renal histology and function. Next, fecal microbiota transplantation experiment verified that the positive effect of PLR on CKD was, at least partially, exerted through gut microbiota reestablishment and downregulation of the Wnt/β‑catenin pathway. The present study provided evidence for a new function of PLR on kidney protection and put forward a potential therapeutic strategy target for CKD.
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Copy and paste a formatted citation
Spandidos Publications style
Wu P, Xue J, Zhu Z, Yu Y, Sun Q, Xie M, Wang B, Huang P, Feng Z, Zhao J, Zhao J, et al: <em>Puerariae lobatae</em> Radix ameliorates chronic kidney disease by reshaping gut microbiota and downregulating Wnt/&beta;‑catenin signaling. Mol Med Rep 30: 117, 2024.
APA
Wu, P., Xue, J., Zhu, Z., Yu, Y., Sun, Q., Xie, M. ... Zhao, J. (2024). <em>Puerariae lobatae</em> Radix ameliorates chronic kidney disease by reshaping gut microbiota and downregulating Wnt/&beta;‑catenin signaling. Molecular Medicine Reports, 30, 117. https://doi.org/10.3892/mmr.2024.13241
MLA
Wu, P., Xue, J., Zhu, Z., Yu, Y., Sun, Q., Xie, M., Wang, B., Huang, P., Feng, Z., Zhao, J."<em>Puerariae lobatae</em> Radix ameliorates chronic kidney disease by reshaping gut microbiota and downregulating Wnt/&beta;‑catenin signaling". Molecular Medicine Reports 30.1 (2024): 117.
Chicago
Wu, P., Xue, J., Zhu, Z., Yu, Y., Sun, Q., Xie, M., Wang, B., Huang, P., Feng, Z., Zhao, J."<em>Puerariae lobatae</em> Radix ameliorates chronic kidney disease by reshaping gut microbiota and downregulating Wnt/&beta;‑catenin signaling". Molecular Medicine Reports 30, no. 1 (2024): 117. https://doi.org/10.3892/mmr.2024.13241
Copy and paste a formatted citation
x
Spandidos Publications style
Wu P, Xue J, Zhu Z, Yu Y, Sun Q, Xie M, Wang B, Huang P, Feng Z, Zhao J, Zhao J, et al: <em>Puerariae lobatae</em> Radix ameliorates chronic kidney disease by reshaping gut microbiota and downregulating Wnt/&beta;‑catenin signaling. Mol Med Rep 30: 117, 2024.
APA
Wu, P., Xue, J., Zhu, Z., Yu, Y., Sun, Q., Xie, M. ... Zhao, J. (2024). <em>Puerariae lobatae</em> Radix ameliorates chronic kidney disease by reshaping gut microbiota and downregulating Wnt/&beta;‑catenin signaling. Molecular Medicine Reports, 30, 117. https://doi.org/10.3892/mmr.2024.13241
MLA
Wu, P., Xue, J., Zhu, Z., Yu, Y., Sun, Q., Xie, M., Wang, B., Huang, P., Feng, Z., Zhao, J."<em>Puerariae lobatae</em> Radix ameliorates chronic kidney disease by reshaping gut microbiota and downregulating Wnt/&beta;‑catenin signaling". Molecular Medicine Reports 30.1 (2024): 117.
Chicago
Wu, P., Xue, J., Zhu, Z., Yu, Y., Sun, Q., Xie, M., Wang, B., Huang, P., Feng, Z., Zhao, J."<em>Puerariae lobatae</em> Radix ameliorates chronic kidney disease by reshaping gut microbiota and downregulating Wnt/&beta;‑catenin signaling". Molecular Medicine Reports 30, no. 1 (2024): 117. https://doi.org/10.3892/mmr.2024.13241
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