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GPX7 reduces chondrocyte inflammation and extracellular matrix degradation triggered by IL‑1β, via a mechanism mediated by ferroptosis

  • Authors:
    • Boyuan Chen
    • Weihao Fu
    • Chunyang Jie
    • Guoxiu Zhang
    • Zhen Li
    • Yihai Liu
    • Shibo Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Physical Education, Henan University of Science and Technology, Luoyang, Henan 471023, P.R. China, Integrative Exercise Physiology Laboratory, Department of Physical Education, Jeonbuk National University, Jeonju 54896, Republic of Korea, Department of General Medicine, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan 471023, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 118
    |
    Published online on: May 14, 2024
       https://doi.org/10.3892/mmr.2024.13242
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Abstract

During osteoarthritis (OA), chondrocytes become highly active, with increased matrix synthesis and inflammatory cytokine‑induced catabolic pathways. Early intervention strategies targeting pathological changes may attenuate or halt disease progression. The present study aimed to reveal the role of glutathione peroxidase (GPX)7 in OA. For this purpose, a research model was established by inducing C28/I2 human chondrocytes with interleukin (IL)‑1β, and the expression level of GPX7 was determined. To explore its roles, C28/I2 cells were transfected to gain GPX7 overexpression. The effects of GPX7 overexpression on intracellular inflammation, extracellular matrix (ECM) degradation, apoptosis and ferroptosis were then evaluated. In addition, the cells were treated with the ferroptosis inducer, erastin, and its effects on the aforementioned phenotypes were assessed. The level of GPX7 was decreased in response to IL‑1β treatment, and GPX7 overexpression suppressed cellular inflammation, ECM degradation and apoptosis. Moreover, the reduction of lipid peroxidation, ferrous ions and transferrin indicated that GPX7 overexpression inhibited ferroptosis. Subsequently, inflammation, ECM degradation and apoptosis were found to be promoted in the cells upon treatment with erastin. These findings suggested that the regulatory role of GPX7 may be mediated by a pathway involving ferroptosis. On the whole, the present study revealed that GPX7 reduces IL‑1β‑induced chondrocyte inflammation, apoptosis and ECM degradation partially through a mechanism involving ferroptosis. The results of the present study lay a theoretical foundation for subsequent OA‑related research and may enable the development of translational strategies for the treatment of OA.
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View References

1 

Yunus MHM, Nordin A and Kamal H: Pathophysiological perspective of osteoarthritis. Medicina (Kaunas). 56:6142020. View Article : Google Scholar : PubMed/NCBI

2 

Yao Q, Wu X, Tao C, Gong W, Chen M, Qu M, Zhong Y, He T, Chen S and Xiao G: Osteoarthritis: Pathogenic signaling pathways and therapeutic targets. Signal Transduct Target Ther. 8:562023. View Article : Google Scholar : PubMed/NCBI

3 

Hawker GA and King LK: The burden of osteoarthritis in older adults. Clin Geriatr Med. 38:181–192. 2022. View Article : Google Scholar : PubMed/NCBI

4 

Katz JN, Arant KR and Loeser RF: Diagnosis and treatment of hip and knee osteoarthritis: A review. JAMA. 325:568–578. 2021. View Article : Google Scholar : PubMed/NCBI

5 

Krause M, Freudenthaler F, Frosch KH, Achtnich A, Petersen W and Akoto R: Operative versus conservative treatment of anterior cruciate ligament rupture. Dtsch Arztebl Int. 115:855–862. 2018.PubMed/NCBI

6 

Molnar V, Matišić V, Kodvanj I, Bjelica R, Jeleč Ž, Hudetz D, Rod E, Čukelj F, Vrdoljak T, Vidović D, et al: Cytokines and chemokines involved in osteoarthritis pathogenesis. Int J Mol Sci. 22:92082021. View Article : Google Scholar : PubMed/NCBI

7 

Xie Y, Kang R, Klionsky DJ and Tang D: GPX4 in cell death, autophagy, and disease. Autophagy. 19:2621–2638. 2023. View Article : Google Scholar : PubMed/NCBI

8 

Kanemura S, Sofia EF, Hirai N, Okumura M, Kadokura H and Inaba K: Characterization of the endoplasmic reticulum-resident peroxidases GPx7 and GPx8 shows the higher oxidative activity of GPx7 and its linkage to oxidative protein folding. J Biol Chem. 295:12772–12785. 2020. View Article : Google Scholar : PubMed/NCBI

9 

Wei PC, Hsieh YH, Su MI, Jiang X, Hsu PH, Lo WT, Weng JY, Jeng YM, Wang JM, Chen PL, et al: Loss of the oxidative stress sensor NPGPx compromises GRP78 chaperone activity and induces systemic disease. Mol Cell. 48:747–759. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Hu X, Li B, Wu F, Liu X, Liu M, Wang C, Shi Y and Ye L: GPX7 Facilitates BMSCs Osteoblastogenesis via ER Stress and mTOR Pathway. J Cell Mol Med. 25:10454–10465. 2021. View Article : Google Scholar : PubMed/NCBI

11 

Kim HJ, Lee Y, Fang S, Kim W, Kim HJ and Kim JW: GPx7 ameliorates non-alcoholic steatohepatitis by regulating oxidative stress. BMB Rep. 53:317–322. 2020. View Article : Google Scholar : PubMed/NCBI

12 

Hwang HS and Shim JH: Brazilin and Caesalpinia sappan L. extract protect epidermal keratinocytes from oxidative stress by inducing the expression of GPX7. Chin J Nat Med. 16:203–209. 2018.PubMed/NCBI

13 

Zhou X, Zheng Y, Sun W, Zhang Z and Liu J, Yang W, Yuan W, Yi Y, Wang J and Liu J: D-mannose alleviates osteoarthritis progression by inhibiting chondrocyte ferroptosis in a HIF-2α-dependent manner. Cell Prolif. 54:e131342021. View Article : Google Scholar : PubMed/NCBI

14 

Zhou Y, Wu H, Wang F, Xu L, Yan Y, Tong X and Yan H: GPX7 Is Targeted by miR-29b and GPX7 knockdown enhances ferroptosis induced by erastin in glioma. Front Oncol. 11:8021242022. View Article : Google Scholar : PubMed/NCBI

15 

Wang BW, Jiang Y, Yao ZL, Chen PS, Yu B and Wang SN: Aucubin protects chondrocytes against IL-1β-Induced apoptosis in vitro and inhibits osteoarthritis in mice model. Drug Des Devel Ther. 13:3529–3538. 2019. View Article : Google Scholar : PubMed/NCBI

16 

Dixon SJ, Lemberg KM, Lamprecht MR, Skouta R, Zaitsev EM, Gleason CE, Patel DN, Bauer AJ, Cantley AM, Yang WS, et al: Ferroptosis: An iron-dependent form of nonapoptotic cell death. Cell. 149:1060–1072. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Guilak F, Nims RJ, Dicks A, Wu CL and Meulenbelt I: Osteoarthritis as a disease of the cartilage pericellular matrix. Matrix Biol. 71–72. 40–50. 2018.

19 

Wilkinson JM and Zeggini E: The genetic epidemiology of joint shape and the development of osteoarthritis. Calcif Tissue Int. 109:257–276. 2021. View Article : Google Scholar : PubMed/NCBI

20 

Cui N, Hu M and Khalil RA: Biochemical and biological attributes of matrix metalloproteinases. Prog Mol Biol Transl Sci. 147:1–73. 2017. View Article : Google Scholar : PubMed/NCBI

21 

Huang J, Zhao L and Chen D: Growth factor signalling in osteoarthritis. Growth Factors. 36:187–195. 2018. View Article : Google Scholar : PubMed/NCBI

22 

Wang T and He C: Pro-inflammatory cytokines: The link between obesity and osteoarthritis. Cytokine Growth Factor Rev. 44:38–50. 2018. View Article : Google Scholar : PubMed/NCBI

23 

Wang J, Chen J, Zhang B and Jia X: IL-6 regulates the bone metabolism and inflammatory microenvironment in aging mice by inhibiting Setd7. Acta Histochem. 123:1517182021. View Article : Google Scholar : PubMed/NCBI

24 

Ashruf OS and Ansari MY: Natural compounds: Potential therapeutics for the inhibition of cartilage matrix degradation in osteoarthritis. Life (Basel). 13:1022022.PubMed/NCBI

25 

Zhu R, Wang Y, Ouyang Z, Hao W, Zhou F, Lin Y, Cheng Y, Zhou R and Hu W: Targeting regulated chondrocyte death in osteoarthritis therapy. Biochem Pharmacol. 215:1157072023. View Article : Google Scholar : PubMed/NCBI

26 

Zhang T and Li J: Experimental study on the ratios of chondrocytes apoptosis in OA. Chin Modern Doctor. 46:35–36. 2008.(In Chinese).

27 

Mou Y, Wang J, Wu J, He D, Zhang C, Duan C and Li B: Ferroptosis, a new form of cell death: Opportunities and challenges in cancer. J Hematol Oncol. 12:342019. View Article : Google Scholar : PubMed/NCBI

28 

Mo Z, Xu P and Li H: Stigmasterol alleviates interleukin-1beta-induced chondrocyte injury by down-regulatingsterol regulatory element binding transcription factor 2 to regulateferroptosis. Bioengineered. 12:9332–9340. 2021. View Article : Google Scholar : PubMed/NCBI

29 

Sun K, Hou L, Guo Z, Wang G, Guo J, Xu J, Zhang X and Guo F: JNK-JUN-NCOA4 axis contributes to chondrocyte ferroptosis and aggravates osteoarthritis via ferritinophagy. Free Radic Biol Med. 200:87–101. 2023. View Article : Google Scholar : PubMed/NCBI

30 

Yang J, Hu S, Bian Y, Yao J, Wang D, Liu X, Guo Z, Zhang S and Peng L: Targeting cell death: Pyroptosis, ferroptosis, apoptosis and necroptosis in osteoarthritis. Front Cell Dev Biol. 9:7899482022. View Article : Google Scholar : PubMed/NCBI

31 

Zhang X, Hou L, Guo Z, Wang G, Xu J, Zheng Z, Sun K and Guo F: Lipid peroxidation in osteoarthritis: Focusing on 4-hydroxynonenal, malondialdehyde, and ferroptosis. Cell Death Discov. 9:3202023. View Article : Google Scholar : PubMed/NCBI

32 

An F, Zhang J, Gao P, Xiao Z, Chang W, Song J, Wang Y, Ma H, Zhang R, Chen Z and Yan C: New insight of the pathogenesis in osteoarthritis: The intricate interplay of ferroptosis and autophagy mediated by mitophagy/chaperone-mediated autophagy. Front Cell Dev Biol. 11:12970242023. View Article : Google Scholar : PubMed/NCBI

33 

Rochette L, Dogon G, Rigal E, Zeller M, Cottin Y and Vergely C: Lipid peroxidation and iron metabolism: Two corner stones in the homeostasis control of ferroptosis. Int J Mol Sci. 24:4492022. View Article : Google Scholar : PubMed/NCBI

34 

Hou Y, Wang S, Jiang L, Sun X, Li J, Wang N, Liu X, Yao X, Zhang C, Deng H and Yang G: Patulin induces acute kidney injury in mice through autophagy-ferroptosis pathway. J Agric Food Chem. 70:6213–6223. 2022. View Article : Google Scholar : PubMed/NCBI

35 

Cotticelli MG, Xia S, Lin D, Lee T, Terrab L, Wipf P, Huryn DM and Wilson RB: Ferroptosis as a novel therapeutic target for friedreich's Ataxia. J Pharmacol Exp Ther. 369:47–54. 2019. View Article : Google Scholar : PubMed/NCBI

36 

Weaver K and Skouta R: The selenoprotein glutathione peroxidase 4: From molecular mechanisms to novel therapeutic opportunities. Biomedicines. 10:8912022. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chen B, Fu W, Jie C, Zhang G, Li Z, Liu Y and Zhou S: GPX7 reduces chondrocyte inflammation and extracellular matrix degradation triggered by IL‑1β, via a mechanism mediated by ferroptosis. Mol Med Rep 30: 118, 2024.
APA
Chen, B., Fu, W., Jie, C., Zhang, G., Li, Z., Liu, Y., & Zhou, S. (2024). GPX7 reduces chondrocyte inflammation and extracellular matrix degradation triggered by IL‑1β, via a mechanism mediated by ferroptosis. Molecular Medicine Reports, 30, 118. https://doi.org/10.3892/mmr.2024.13242
MLA
Chen, B., Fu, W., Jie, C., Zhang, G., Li, Z., Liu, Y., Zhou, S."GPX7 reduces chondrocyte inflammation and extracellular matrix degradation triggered by IL‑1β, via a mechanism mediated by ferroptosis". Molecular Medicine Reports 30.1 (2024): 118.
Chicago
Chen, B., Fu, W., Jie, C., Zhang, G., Li, Z., Liu, Y., Zhou, S."GPX7 reduces chondrocyte inflammation and extracellular matrix degradation triggered by IL‑1β, via a mechanism mediated by ferroptosis". Molecular Medicine Reports 30, no. 1 (2024): 118. https://doi.org/10.3892/mmr.2024.13242
Copy and paste a formatted citation
x
Spandidos Publications style
Chen B, Fu W, Jie C, Zhang G, Li Z, Liu Y and Zhou S: GPX7 reduces chondrocyte inflammation and extracellular matrix degradation triggered by IL‑1β, via a mechanism mediated by ferroptosis. Mol Med Rep 30: 118, 2024.
APA
Chen, B., Fu, W., Jie, C., Zhang, G., Li, Z., Liu, Y., & Zhou, S. (2024). GPX7 reduces chondrocyte inflammation and extracellular matrix degradation triggered by IL‑1β, via a mechanism mediated by ferroptosis. Molecular Medicine Reports, 30, 118. https://doi.org/10.3892/mmr.2024.13242
MLA
Chen, B., Fu, W., Jie, C., Zhang, G., Li, Z., Liu, Y., Zhou, S."GPX7 reduces chondrocyte inflammation and extracellular matrix degradation triggered by IL‑1β, via a mechanism mediated by ferroptosis". Molecular Medicine Reports 30.1 (2024): 118.
Chicago
Chen, B., Fu, W., Jie, C., Zhang, G., Li, Z., Liu, Y., Zhou, S."GPX7 reduces chondrocyte inflammation and extracellular matrix degradation triggered by IL‑1β, via a mechanism mediated by ferroptosis". Molecular Medicine Reports 30, no. 1 (2024): 118. https://doi.org/10.3892/mmr.2024.13242
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