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Astragaloside IV protects against autoimmune myasthenia gravis in rats via regulation of mitophagy and apoptosis

  • Authors:
    • Jingjing Zhang
    • Jiayan Huang
    • Jinlian Lan
    • Qing Li
    • Lingling Ke
    • Qilong Jiang
    • Yanwu Li
    • Han Zhang
    • Huiya Zhong
    • Peidan Yang
    • Tongkai Chen
    • Yafang Song
  • View Affiliations / Copyright

    Affiliations: Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510405, P.R. China, Department of Gastrosplenic Diseases, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510405, P.R. China, School of Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 129
    |
    Published online on: May 24, 2024
       https://doi.org/10.3892/mmr.2024.13253
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Abstract

Astragaloside IV (AS‑IV) has various pharmacological effects, including antioxidant and immunoregulatory properties, which can improve myasthenia gravis (MG) symptoms. However, the potential mechanism underlying the effects of AS‑IV on MG remains to be elucidated. The present study aimed to investigate whether AS‑IV has a therapeutic effect on MG and its potential mechanism of action. By subcutaneously immunizing rats with R97‑116 peptide, an experimental autoimmune (EA) MG rat model was established. AS‑IV (40 or 80 mg/kg/day) treatment was then applied for 28 days after modeling. The results demonstrated that AS‑IV significantly ameliorated the weight loss, Lennon score and pathological changes in the gastrocnemius muscle of EAMG rats compared with the model group. Additionally, the levels of acetylcholine receptor antibody (AChR‑Ab) were significantly decreased, whereas mitochondrial function [ATPase and cytochrome c (Cyt‑C) oxidase activities] and ultrastructure were improved in the AS‑IV treated rats. Moreover, the mRNA and protein expression levels of phosphatase and tensin homolog‑induced putative kinase 1, Parkin, LC3II and Bcl‑2, key signaling molecules for mitophagy and apoptosis, were upregulated, whereas the mRNA and protein expression levels of p62, Cyt‑C, Bax, caspase 3 and caspase 9 were downregulated following AS‑IV intervention. In conclusion, AS‑IV may protect against EAMG in a rat model by modulating mitophagy and apoptosis. These findings indicated the potential mechanism underlying the effects of AS‑IV on MG and provided novel insights into treatment strategies for MG.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang J, Huang J, Lan J, Li Q, Ke L, Jiang Q, Li Y, Zhang H, Zhong H, Yang P, Yang P, et al: Astragaloside IV protects against autoimmune myasthenia gravis in rats via regulation of mitophagy and apoptosis. Mol Med Rep 30: 129, 2024.
APA
Zhang, J., Huang, J., Lan, J., Li, Q., Ke, L., Jiang, Q. ... Song, Y. (2024). Astragaloside IV protects against autoimmune myasthenia gravis in rats via regulation of mitophagy and apoptosis. Molecular Medicine Reports, 30, 129. https://doi.org/10.3892/mmr.2024.13253
MLA
Zhang, J., Huang, J., Lan, J., Li, Q., Ke, L., Jiang, Q., Li, Y., Zhang, H., Zhong, H., Yang, P., Chen, T., Song, Y."Astragaloside IV protects against autoimmune myasthenia gravis in rats via regulation of mitophagy and apoptosis". Molecular Medicine Reports 30.1 (2024): 129.
Chicago
Zhang, J., Huang, J., Lan, J., Li, Q., Ke, L., Jiang, Q., Li, Y., Zhang, H., Zhong, H., Yang, P., Chen, T., Song, Y."Astragaloside IV protects against autoimmune myasthenia gravis in rats via regulation of mitophagy and apoptosis". Molecular Medicine Reports 30, no. 1 (2024): 129. https://doi.org/10.3892/mmr.2024.13253
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang J, Huang J, Lan J, Li Q, Ke L, Jiang Q, Li Y, Zhang H, Zhong H, Yang P, Yang P, et al: Astragaloside IV protects against autoimmune myasthenia gravis in rats via regulation of mitophagy and apoptosis. Mol Med Rep 30: 129, 2024.
APA
Zhang, J., Huang, J., Lan, J., Li, Q., Ke, L., Jiang, Q. ... Song, Y. (2024). Astragaloside IV protects against autoimmune myasthenia gravis in rats via regulation of mitophagy and apoptosis. Molecular Medicine Reports, 30, 129. https://doi.org/10.3892/mmr.2024.13253
MLA
Zhang, J., Huang, J., Lan, J., Li, Q., Ke, L., Jiang, Q., Li, Y., Zhang, H., Zhong, H., Yang, P., Chen, T., Song, Y."Astragaloside IV protects against autoimmune myasthenia gravis in rats via regulation of mitophagy and apoptosis". Molecular Medicine Reports 30.1 (2024): 129.
Chicago
Zhang, J., Huang, J., Lan, J., Li, Q., Ke, L., Jiang, Q., Li, Y., Zhang, H., Zhong, H., Yang, P., Chen, T., Song, Y."Astragaloside IV protects against autoimmune myasthenia gravis in rats via regulation of mitophagy and apoptosis". Molecular Medicine Reports 30, no. 1 (2024): 129. https://doi.org/10.3892/mmr.2024.13253
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