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MicroRNA‑17‑5p alleviates sepsis‑related acute kidney injury in mice by modulating inflammation and apoptosis

  • Authors:
    • Jian Sun
    • Lei Niu
    • Yang Wang
    • Gang Zhao
    • Lujia Tang
    • Jiamei Jiang
    • Shuming Pan
    • Xiaoli Ge
  • View Affiliations / Copyright

    Affiliations: Emergency Department, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, P.R. China, Department of Emergency Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 20023, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 139
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    Published online on: June 14, 2024
       https://doi.org/10.3892/mmr.2024.13263
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Abstract

Septic acute kidney injury (AKI) is considered as a severe and frequent complication that occurs during sepsis. Mounting evidence has confirmed the pivotal pathogenetic roles of microRNA (miRNA or miR) in sepsis‑induced AKI; however, the role of miRNAs and their underlying mechanisms in sepsis‑induced AKI have not been entirely understood. The present study aimed to elucidate the functions of special miRNAs during sepsis‑induced AKI and its underlying mechanism. First, a number of differently expressed miRNAs was identified based on the microarray dataset GSE172044. Subsequently, lipopolysaccharide (LPS) was used to induce AKI in mice, and the role of miR‑17‑5p on AKI was clarified. Finally, the related molecular mechanisms were further examined by western blotting and immunohistochemical analysis. MiR‑17‑5p was found to be continuously decreased and reached the bottom at h 24 after AKI in mice. Functionally, injection of agomiR‑17‑5p could observably improve renal injury and survival rate, as well as inhibit inflammatory cytokine production and renal cell apoptosis in mice after AKI. On the contrary, injection of antagomiR‑17‑5p aggravated LPS‑induced renal injury, inflammation and apoptosis in mice after AKI. Moreover, transforming growth factor β receptor 2 (TGFβR2) was identified as a direct target of miR‑17‑5p, and its downstream phosphorylated Smad3 was also suppressed by miR‑17‑5p upregulation. Taken together, these results demonstrated that miR‑17‑5p overexpression may exhibit a beneficial effect by attenuating LPS‑induced inflammation and apoptosis via regulating the TGFβR2/TGF‑β/Smad3 signaling pathway, indicating that miR‑17‑5p could act as a potential target for sepsis treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Sun J, Niu L, Wang Y, Zhao G, Tang L, Jiang J, Pan S and Ge X: MicroRNA‑17‑5p alleviates sepsis‑related acute kidney injury in mice by modulating inflammation and apoptosis. Mol Med Rep 30: 139, 2024.
APA
Sun, J., Niu, L., Wang, Y., Zhao, G., Tang, L., Jiang, J. ... Ge, X. (2024). MicroRNA‑17‑5p alleviates sepsis‑related acute kidney injury in mice by modulating inflammation and apoptosis. Molecular Medicine Reports, 30, 139. https://doi.org/10.3892/mmr.2024.13263
MLA
Sun, J., Niu, L., Wang, Y., Zhao, G., Tang, L., Jiang, J., Pan, S., Ge, X."MicroRNA‑17‑5p alleviates sepsis‑related acute kidney injury in mice by modulating inflammation and apoptosis". Molecular Medicine Reports 30.2 (2024): 139.
Chicago
Sun, J., Niu, L., Wang, Y., Zhao, G., Tang, L., Jiang, J., Pan, S., Ge, X."MicroRNA‑17‑5p alleviates sepsis‑related acute kidney injury in mice by modulating inflammation and apoptosis". Molecular Medicine Reports 30, no. 2 (2024): 139. https://doi.org/10.3892/mmr.2024.13263
Copy and paste a formatted citation
x
Spandidos Publications style
Sun J, Niu L, Wang Y, Zhao G, Tang L, Jiang J, Pan S and Ge X: MicroRNA‑17‑5p alleviates sepsis‑related acute kidney injury in mice by modulating inflammation and apoptosis. Mol Med Rep 30: 139, 2024.
APA
Sun, J., Niu, L., Wang, Y., Zhao, G., Tang, L., Jiang, J. ... Ge, X. (2024). MicroRNA‑17‑5p alleviates sepsis‑related acute kidney injury in mice by modulating inflammation and apoptosis. Molecular Medicine Reports, 30, 139. https://doi.org/10.3892/mmr.2024.13263
MLA
Sun, J., Niu, L., Wang, Y., Zhao, G., Tang, L., Jiang, J., Pan, S., Ge, X."MicroRNA‑17‑5p alleviates sepsis‑related acute kidney injury in mice by modulating inflammation and apoptosis". Molecular Medicine Reports 30.2 (2024): 139.
Chicago
Sun, J., Niu, L., Wang, Y., Zhao, G., Tang, L., Jiang, J., Pan, S., Ge, X."MicroRNA‑17‑5p alleviates sepsis‑related acute kidney injury in mice by modulating inflammation and apoptosis". Molecular Medicine Reports 30, no. 2 (2024): 139. https://doi.org/10.3892/mmr.2024.13263
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