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Protective effects of fermented Rosa roxburghii Tratt juice against ethanol‑induced hepatocyte injury by regulating the NRF2‑AMPK signaling pathway in AML‑12 cells

  • Authors:
    • Lalai Zikela
    • Zhuoli Yu
    • Jindan Han
    • Huilin Zhu
    • Dingli Wang
    • Xuezhu Wang
    • Songtao Li
    • Qiang Han
  • View Affiliations / Copyright

    Affiliations: School of Public Health, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China, School of Food Science and Engineering, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, P.R. China
    Copyright: © Zikela et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 174
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    Published online on: July 31, 2024
       https://doi.org/10.3892/mmr.2024.13298
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Abstract

Alcohol‑related liver disease (ALD) is a major health concern worldwide. In recent years, there has been growing interest in natural products and functional foods for preventing and treating ALD due to their potential antioxidant and hepatoprotective properties. Rosa roxburghii Tratt, known for its rich content of bioactive compounds, has demonstrated promising health benefits, including anti‑inflammatory and antioxidant effects. Fermentation has been utilized as a strategy to enhance the bioavailability and efficacy of natural products. In the present study, using a mixture of Rosa roxburghii Tratt juice, lotus leaf extract and grape seed proanthocyanidins fermented by Lactobacillus plantarum HH‑LP56, a novel fermented Rosa roxburghii Tratt (FRRT) juice was discovered that can prevent and regulate ethanol‑induced liver cell damage. Following fermentation, the pH was significantly decreased, and the content of VC and superoxide dismutase (SOD) were significantly increased, along with a noticeable enhancement in hydroxyl and 2,2‑diphenyl‑1‑picrylhydrazyl free radical scavenging abilities. Alpha Mouse liver 12 cells were exposed to ethanol for 24 h to establish an in vitro liver cell injury model. The present study evaluated the effects of FRRT on cell damage, lipid accumulation and oxidative stress markers. The results revealed that FRRT pretreatment (cells were pre‑treated with 2.5 and 5 mg/ml FRRT for 2 h) significantly reduced lipid accumulation and oxidative stress in liver cells. Mechanistically, FRRT regulated lipid metabolism by influencing key genes and proteins, such as AMP‑activated protein kinase, sterol regulatory element binding transcription factor 1 and Stearyl‑CoA desaturase‑1. Furthermore, FRRT enhanced antioxidant activity by increasing SOD activity, glutathione and catalase levels, while reducing reactive oxygen species and malondialdehyde levels. It also reversed the expression changes of ethanol‑induced oxidative stress‑related genes and proteins. In conclusion, a novel functional food ingredient may have been discovered with extensive potential applications. These findings indicated that FRRT has antioxidant properties and potential therapeutic benefits in addressing ethanol‑induced liver cell damage through its effects on liver lipid metabolism and oxidative stress.
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Copy and paste a formatted citation
Spandidos Publications style
Zikela L, Yu Z, Han J, Zhu H, Wang D, Wang X, Li S and Han Q: Protective effects of fermented <em>Rosa roxburghii Tratt</em> juice against ethanol‑induced hepatocyte injury by regulating the NRF2‑AMPK signaling pathway in AML‑12 cells. Mol Med Rep 30: 174, 2024.
APA
Zikela, L., Yu, Z., Han, J., Zhu, H., Wang, D., Wang, X. ... Han, Q. (2024). Protective effects of fermented <em>Rosa roxburghii Tratt</em> juice against ethanol‑induced hepatocyte injury by regulating the NRF2‑AMPK signaling pathway in AML‑12 cells. Molecular Medicine Reports, 30, 174. https://doi.org/10.3892/mmr.2024.13298
MLA
Zikela, L., Yu, Z., Han, J., Zhu, H., Wang, D., Wang, X., Li, S., Han, Q."Protective effects of fermented <em>Rosa roxburghii Tratt</em> juice against ethanol‑induced hepatocyte injury by regulating the NRF2‑AMPK signaling pathway in AML‑12 cells". Molecular Medicine Reports 30.4 (2024): 174.
Chicago
Zikela, L., Yu, Z., Han, J., Zhu, H., Wang, D., Wang, X., Li, S., Han, Q."Protective effects of fermented <em>Rosa roxburghii Tratt</em> juice against ethanol‑induced hepatocyte injury by regulating the NRF2‑AMPK signaling pathway in AML‑12 cells". Molecular Medicine Reports 30, no. 4 (2024): 174. https://doi.org/10.3892/mmr.2024.13298
Copy and paste a formatted citation
x
Spandidos Publications style
Zikela L, Yu Z, Han J, Zhu H, Wang D, Wang X, Li S and Han Q: Protective effects of fermented <em>Rosa roxburghii Tratt</em> juice against ethanol‑induced hepatocyte injury by regulating the NRF2‑AMPK signaling pathway in AML‑12 cells. Mol Med Rep 30: 174, 2024.
APA
Zikela, L., Yu, Z., Han, J., Zhu, H., Wang, D., Wang, X. ... Han, Q. (2024). Protective effects of fermented <em>Rosa roxburghii Tratt</em> juice against ethanol‑induced hepatocyte injury by regulating the NRF2‑AMPK signaling pathway in AML‑12 cells. Molecular Medicine Reports, 30, 174. https://doi.org/10.3892/mmr.2024.13298
MLA
Zikela, L., Yu, Z., Han, J., Zhu, H., Wang, D., Wang, X., Li, S., Han, Q."Protective effects of fermented <em>Rosa roxburghii Tratt</em> juice against ethanol‑induced hepatocyte injury by regulating the NRF2‑AMPK signaling pathway in AML‑12 cells". Molecular Medicine Reports 30.4 (2024): 174.
Chicago
Zikela, L., Yu, Z., Han, J., Zhu, H., Wang, D., Wang, X., Li, S., Han, Q."Protective effects of fermented <em>Rosa roxburghii Tratt</em> juice against ethanol‑induced hepatocyte injury by regulating the NRF2‑AMPK signaling pathway in AML‑12 cells". Molecular Medicine Reports 30, no. 4 (2024): 174. https://doi.org/10.3892/mmr.2024.13298
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