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Adiponectin receptor 1 regulates endometrial receptivity via the adenosine monophosphate‑activated protein kinase/E‑cadherin pathway

  • Authors:
    • Bolor-Erdene Sarankhuu
    • Hye Jin Jeon
    • Da-Un Jeong
    • Seok-Rae Park
    • Tae-Hyun Kim
    • Sung Ki Lee
    • Ae Ra Han
    • Seong-Lan Yu
    • Jaeku Kang
  • View Affiliations / Copyright

    Affiliations: Priority Research Center, Myunggok Medical Research Institute, College of Medicine, Konyang University, Daejeon 35365, Republic of Korea, I‑Dream Clinic, Department of Obstetrics and Gynecology, Mizmedi Hospital, Seoul 07639, Republic of Korea
    Copyright: © Sarankhuu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 184
    |
    Published online on: August 16, 2024
       https://doi.org/10.3892/mmr.2024.13308
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Abstract

Endometrial receptivity is essential for successful embryo implantation and pregnancy initiation and is regulated via various signaling pathways. Adiponectin, an important adipokine, may be a potential regulator of reproductive system functions. The aim of the present study was to elucidate the regulatory role of adiponectin receptor 1 (ADIPOR1) in endometrial receptivity. The endometrial receptivity between RL95‑2 and AN3CA cell lines was confirmed using an in vitro JAr spheroid attachment model. 293T cells were transfected with control or short hairpin (sh)ADIPOR1 vectors and RL95‑2 cells were transduced with lentiviral particles targeting ADIPOR1. Reverse transcription‑quantitative PCR and immunoblot assays were also performed. ADIPOR1 was consistently upregulated in the endometrium during the mid‑secretory phase compared with that in the proliferative phase and in receptive RL95‑2 cells compared with that in non‑receptive AN3CA cells. Stable cell lines with diminished ADIPOR1 expression caused by shRNA showed reduced E‑cadherin expression and attenuated in vitro endometrial receptivity. ADIPOR1 regulated AMP‑activated protein kinase (AMPK) activity in endometrial epithelial cells. Regulation of AMPK activity via dorsomorphin and 5‑aminoimidazole‑4‑carboxamide ribonucleotide affected E‑cadherin expression and in vitro endometrial receptivity. The ADIPOR1/AMPK/E‑cadherin axis is vital to endometrial receptivity. These findings can help improve fertility treatments and outcomes.
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Copy and paste a formatted citation
Spandidos Publications style
Sarankhuu B, Jeon HJ, Jeong D, Park S, Kim T, Lee SK, Han AR, Yu S and Kang J: Adiponectin receptor 1 regulates endometrial receptivity via the adenosine monophosphate‑activated protein kinase/E‑cadherin pathway. Mol Med Rep 30: 184, 2024.
APA
Sarankhuu, B., Jeon, H.J., Jeong, D., Park, S., Kim, T., Lee, S.K. ... Kang, J. (2024). Adiponectin receptor 1 regulates endometrial receptivity via the adenosine monophosphate‑activated protein kinase/E‑cadherin pathway. Molecular Medicine Reports, 30, 184. https://doi.org/10.3892/mmr.2024.13308
MLA
Sarankhuu, B., Jeon, H. J., Jeong, D., Park, S., Kim, T., Lee, S. K., Han, A. R., Yu, S., Kang, J."Adiponectin receptor 1 regulates endometrial receptivity via the adenosine monophosphate‑activated protein kinase/E‑cadherin pathway". Molecular Medicine Reports 30.4 (2024): 184.
Chicago
Sarankhuu, B., Jeon, H. J., Jeong, D., Park, S., Kim, T., Lee, S. K., Han, A. R., Yu, S., Kang, J."Adiponectin receptor 1 regulates endometrial receptivity via the adenosine monophosphate‑activated protein kinase/E‑cadherin pathway". Molecular Medicine Reports 30, no. 4 (2024): 184. https://doi.org/10.3892/mmr.2024.13308
Copy and paste a formatted citation
x
Spandidos Publications style
Sarankhuu B, Jeon HJ, Jeong D, Park S, Kim T, Lee SK, Han AR, Yu S and Kang J: Adiponectin receptor 1 regulates endometrial receptivity via the adenosine monophosphate‑activated protein kinase/E‑cadherin pathway. Mol Med Rep 30: 184, 2024.
APA
Sarankhuu, B., Jeon, H.J., Jeong, D., Park, S., Kim, T., Lee, S.K. ... Kang, J. (2024). Adiponectin receptor 1 regulates endometrial receptivity via the adenosine monophosphate‑activated protein kinase/E‑cadherin pathway. Molecular Medicine Reports, 30, 184. https://doi.org/10.3892/mmr.2024.13308
MLA
Sarankhuu, B., Jeon, H. J., Jeong, D., Park, S., Kim, T., Lee, S. K., Han, A. R., Yu, S., Kang, J."Adiponectin receptor 1 regulates endometrial receptivity via the adenosine monophosphate‑activated protein kinase/E‑cadherin pathway". Molecular Medicine Reports 30.4 (2024): 184.
Chicago
Sarankhuu, B., Jeon, H. J., Jeong, D., Park, S., Kim, T., Lee, S. K., Han, A. R., Yu, S., Kang, J."Adiponectin receptor 1 regulates endometrial receptivity via the adenosine monophosphate‑activated protein kinase/E‑cadherin pathway". Molecular Medicine Reports 30, no. 4 (2024): 184. https://doi.org/10.3892/mmr.2024.13308
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