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Molecular Medicine Reports
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Print ISSN: 1791-2997 Online ISSN: 1791-3004
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January-February 2009 Volume 2 Issue 1

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

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Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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Article

Single nucleotide polymorphism typing of the human toll-like receptor 4 gene at the 2-kb upstream region of the 5' untranslated region: New enclosure strategy for the risk grouping of poorly-differentiated gastric adenocarcinoma patients

  • Authors:
    • Tadashi Ohara
    • Yuhsaku Kanoh
    • Norio Tani
    • Hironori Ohdaira
    • Yutaka Suzuki
    • Jin-Ichi Kameyama
    • Keiichi Yoshino
    • Masaki Kitajima
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, International University of Health and Welfare Hospital, Tochigi 329-2763, Japan. tohara@iuhw.ac.jp
  • Pages: 17-21
    |
    Published online on: January 1, 2009
       https://doi.org/10.3892/mmr_00000055
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Abstract

To date, no enclosure method for risk grouping patients with poorly-differentiated gastric adenocarcinoma has been identified. We examined the relationship between mutations in toll-like receptor 4 (TLR4) and patients with poorly-differentiated gastric adenocarcinoma. Genomic DNA was extracted from the peripheral blood of 38 patients, 20 with well-differentiated and 18 with poorly-differentiated gastric cancer, from 25 patients with colorectal cancer and from 10 healthy volunteers. The polymorphism of TLR4 up to the 2-kb upstream region of the 5' untranslated region (UTR) was analyzed. The results revealed the presence of single nucleotide polymorphisms (SNPs) only among patients with poorly-differentiated gastric adenocarcinoma. SNPs were found at 3 sites: −2081, −2026 and −1601 in 12, 15 and 15 of the 18 cases of poorly-differentiated gastric adenocarcinoma, respectively. The results of the determination of a consensus among the base sequences of the core promoter, basal promoter and upstream promoter elements reveal that the variant sites were present in the TLR4 mRNA promoter region, suggesting that they were biologically significant variations. Polymorphism analysis of the upstream region of the 5' UTR of TLR4 may be a useful new enclosure strategy for the risk grouping of poorly-differentiated gastric adenocarcinoma patients.

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Copy and paste a formatted citation
Spandidos Publications style
Ohara T, Kanoh Y, Tani N, Ohdaira H, Suzuki Y, Kameyama J, Yoshino K and Kitajima M: Single nucleotide polymorphism typing of the human toll-like receptor 4 gene at the 2-kb upstream region of the 5' untranslated region: New enclosure strategy for the risk grouping of poorly-differentiated gastric adenocarcinoma patients. Mol Med Rep 2: 17-21, 2009.
APA
Ohara, T., Kanoh, Y., Tani, N., Ohdaira, H., Suzuki, Y., Kameyama, J. ... Kitajima, M. (2009). Single nucleotide polymorphism typing of the human toll-like receptor 4 gene at the 2-kb upstream region of the 5' untranslated region: New enclosure strategy for the risk grouping of poorly-differentiated gastric adenocarcinoma patients. Molecular Medicine Reports, 2, 17-21. https://doi.org/10.3892/mmr_00000055
MLA
Ohara, T., Kanoh, Y., Tani, N., Ohdaira, H., Suzuki, Y., Kameyama, J., Yoshino, K., Kitajima, M."Single nucleotide polymorphism typing of the human toll-like receptor 4 gene at the 2-kb upstream region of the 5' untranslated region: New enclosure strategy for the risk grouping of poorly-differentiated gastric adenocarcinoma patients". Molecular Medicine Reports 2.1 (2009): 17-21.
Chicago
Ohara, T., Kanoh, Y., Tani, N., Ohdaira, H., Suzuki, Y., Kameyama, J., Yoshino, K., Kitajima, M."Single nucleotide polymorphism typing of the human toll-like receptor 4 gene at the 2-kb upstream region of the 5' untranslated region: New enclosure strategy for the risk grouping of poorly-differentiated gastric adenocarcinoma patients". Molecular Medicine Reports 2, no. 1 (2009): 17-21. https://doi.org/10.3892/mmr_00000055
Copy and paste a formatted citation
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Spandidos Publications style
Ohara T, Kanoh Y, Tani N, Ohdaira H, Suzuki Y, Kameyama J, Yoshino K and Kitajima M: Single nucleotide polymorphism typing of the human toll-like receptor 4 gene at the 2-kb upstream region of the 5' untranslated region: New enclosure strategy for the risk grouping of poorly-differentiated gastric adenocarcinoma patients. Mol Med Rep 2: 17-21, 2009.
APA
Ohara, T., Kanoh, Y., Tani, N., Ohdaira, H., Suzuki, Y., Kameyama, J. ... Kitajima, M. (2009). Single nucleotide polymorphism typing of the human toll-like receptor 4 gene at the 2-kb upstream region of the 5' untranslated region: New enclosure strategy for the risk grouping of poorly-differentiated gastric adenocarcinoma patients. Molecular Medicine Reports, 2, 17-21. https://doi.org/10.3892/mmr_00000055
MLA
Ohara, T., Kanoh, Y., Tani, N., Ohdaira, H., Suzuki, Y., Kameyama, J., Yoshino, K., Kitajima, M."Single nucleotide polymorphism typing of the human toll-like receptor 4 gene at the 2-kb upstream region of the 5' untranslated region: New enclosure strategy for the risk grouping of poorly-differentiated gastric adenocarcinoma patients". Molecular Medicine Reports 2.1 (2009): 17-21.
Chicago
Ohara, T., Kanoh, Y., Tani, N., Ohdaira, H., Suzuki, Y., Kameyama, J., Yoshino, K., Kitajima, M."Single nucleotide polymorphism typing of the human toll-like receptor 4 gene at the 2-kb upstream region of the 5' untranslated region: New enclosure strategy for the risk grouping of poorly-differentiated gastric adenocarcinoma patients". Molecular Medicine Reports 2, no. 1 (2009): 17-21. https://doi.org/10.3892/mmr_00000055
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