Effect of chelating agents on the growth, surface polypeptide synthesis and interaction of Streptococcus agalactiae with human epithelial cells

  • Authors:
    • Michelle Hanthequeste Bittencourt Dos Santos
    • Andréia Ferreira Eduardo Da Costa
    • Gabriela Da Silva Santos
    • André Luis Souza Dos Santos
    • Prescilla Emy Nagao
  • View Affiliations

  • Published online on: January 1, 2009     https://doi.org/10.3892/mmr_00000065
  • Pages: 81-84
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Abstract

In the present study, the influence of the chelating agents of ethylenediaminetetraacetic acid (EDTA), ethylene glycol-bis (β-aminoethyl ether) (EGTA) and 1,10-phenanthroline (PHEN) on growth rate, cytoadherence ability and surface protein expression was examined in a clinical strain of Streptococcus agalactiae isolated from a blood sample. EDTA and EGTA at 10 mM did not inhibit the growth of S. agalactiae, while PHEN significantly arrested bacterial proliferation in a concentration range of 10-0.01 mM. The in vitro interaction between S. agalactiae and A549 cells was a time-dependent process; adherence and bacterial intracellular viability were more pronounced after 3 h of contact. The pre-treatment of bacterial cells with EDTA adversely influenced the adhesive properties of S. agalactiae to A549 cells after 2 and 3 h, whereas EGTA only blocked this process after 3 h. Viable intracellular bacteria were just detected after 3 h of interaction, and EDTA and EGTA inhibited intracellular viability in a similar fashion. Conversely, PHEN inhibited neither the adherence nor the intracellular viability of the microorganism. Furthermore, EDTA robustly suppressed surface polypeptide synthesis, suggesting a decline in the possible bacterial ligands responsible for S. agalactiae adhesive properties.

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January-February 2009
Volume 2 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Dos Santos MH, Da Costa AF, Da Silva Santos G, Dos Santos AL and Nagao PE: Effect of chelating agents on the growth, surface polypeptide synthesis and interaction of Streptococcus agalactiae with human epithelial cells. Mol Med Rep 2: 81-84, 2009.
APA
Dos Santos, M.H., Da Costa, A.F., Da Silva Santos, G., Dos Santos, A.L., & Nagao, P.E. (2009). Effect of chelating agents on the growth, surface polypeptide synthesis and interaction of Streptococcus agalactiae with human epithelial cells. Molecular Medicine Reports, 2, 81-84. https://doi.org/10.3892/mmr_00000065
MLA
Dos Santos, M. H., Da Costa, A. F., Da Silva Santos, G., Dos Santos, A. L., Nagao, P. E."Effect of chelating agents on the growth, surface polypeptide synthesis and interaction of Streptococcus agalactiae with human epithelial cells". Molecular Medicine Reports 2.1 (2009): 81-84.
Chicago
Dos Santos, M. H., Da Costa, A. F., Da Silva Santos, G., Dos Santos, A. L., Nagao, P. E."Effect of chelating agents on the growth, surface polypeptide synthesis and interaction of Streptococcus agalactiae with human epithelial cells". Molecular Medicine Reports 2, no. 1 (2009): 81-84. https://doi.org/10.3892/mmr_00000065