Astragaloside IV increases MMP-2 mRNA and protein expression in human lung cancer A549 cells

  • Authors:
    • Chin-Cheng Su
    • Tsrong-Laang Chiou
    • Ming-Huan Chan
    • Jaung-Geng Lin
  • View Affiliations

  • Published online on: January 1, 2009     https://doi.org/10.3892/mmr_00000070
  • Pages: 107-113
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Abstract

Lung cancer is the leading cause of cancer-related death in Taiwan. In the clinical treatment of lung cancer patients in Asia, an Astragalus-based herbal mixture is commonly used in conjunction with chemotherapy. The principal component of Astragalus (also known as Huang-qi) is Astragaloside IV. The Astragaloside IV marker has been qualified for the phytochemicals of Astragalus. The recurrence and metastasis of lung cancer are positively correlated with matrix metalloproteinase-2 (MMP-2) mRNA and protein expression. This study examined the effects of Astragaloside IV on the mRNA and protein expression of MMP-2 in lung cancer A549 cells by RT-PCR and Western blotting. The cytotoxicity of Astragaloside IV in these cells was determined using CellTiter 96® AQueous One Solution Reagent and the MTT assay. A549 cells were treated for different durations with Astragaloside IV concentrations of 10, 20, 50 and 100 ng/ml. The respective proliferation rates per amount (relative to the control) were as follows: 24 h, 96.85±1.12, 95.63±0.83, 93.92±0.84, 95.27±0.57%; 48 h, 98.86±1.56, 95.71±0.59, 94.09±0.68, 93.44±0.5%; 72 h, 99.48±0.16, 95.60±0.48, 95.23±0.67, 94.72±1.12%. MMP-2 mRNA expression as well as vascular endothelial growth factor mRNA expression were upregulated at concentrations of 10, 20 and 50 ng/ml. Additionally, protein expression of MMP-2 was increased at concentrations of 10, 20 and 30 µg/ml after 24 h of treatment. These results indicate that Astragaloside IV upregulates MMP-2 mRNA and protein expression in A549 cells, and therefore that it may increase recurrence and metastatic rates in lung cancer. This issue should be further examined in the clinical setting.

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January-February 2009
Volume 2 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Su C, Chiou T, Chan M and Lin J: Astragaloside IV increases MMP-2 mRNA and protein expression in human lung cancer A549 cells. Mol Med Rep 2: 107-113, 2009
APA
Su, C., Chiou, T., Chan, M., & Lin, J. (2009). Astragaloside IV increases MMP-2 mRNA and protein expression in human lung cancer A549 cells. Molecular Medicine Reports, 2, 107-113. https://doi.org/10.3892/mmr_00000070
MLA
Su, C., Chiou, T., Chan, M., Lin, J."Astragaloside IV increases MMP-2 mRNA and protein expression in human lung cancer A549 cells". Molecular Medicine Reports 2.1 (2009): 107-113.
Chicago
Su, C., Chiou, T., Chan, M., Lin, J."Astragaloside IV increases MMP-2 mRNA and protein expression in human lung cancer A549 cells". Molecular Medicine Reports 2, no. 1 (2009): 107-113. https://doi.org/10.3892/mmr_00000070