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Effects of sinomenine on proliferation and apoptosis in human lung cancer cell line NCI-H460 in vitro

  • Authors:
    • Tingshu Jiang
    • Liping Zhou
    • Wenlu Zhang
    • Dan Qu
    • Xiaoman Xu
    • Ying Yang
    • Shengqi Li

  • View Affiliations

  • Published online on: January 1, 2010     https://doi.org/10.3892/mmr_00000217
  • Pages: 51-56
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
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Abstract

Sinomenine (SIN) is an herbal ingredient isolated from Sinomenium acutum Rehd. et Wils. We evaluated the effects of SIN on the growth and apoptosis of human lung cancer NCI-H460 cells. SIN inhibited the proliferation of the cells in a dose- and time-dependent manner. Apoptosis was confirmed by flow cytometry, the TUNEL assay and transmission electron microscopy. SIN-induced apoptosis was accompanied by the collapse of mitochondrial membrane potential, the release of cytochrome C and the activation of caspase-9 and -3. SIN also increased Bax and decreased Bcl-2 protein levels in the NCI-H460 cells. Data analysis suggested that SIN exerted a significant apoptotic effect on NCI-H460 cells through the mitochondrial pathway. SIN is therefore a promising chemopreventive candidate for lung carcinoma.

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January-February 2010
Volume 3 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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  • Journal Metrics
  • Impact Factor: 3.4
  • Ranked Medicine Research and Experimental
    (total number of cites)
  • CiteScore: 6.9
  • CiteScore Rank: Q2: #98/328 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.721
  • SCImago Journal Rank (SJR): 0.65
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Spandidos Publications style
Jiang T, Zhou L, Zhang W, Qu D, Xu X, Yang Y and Li S: Effects of sinomenine on proliferation and apoptosis in human lung cancer cell line NCI-H460 in vitro . Mol Med Rep 3: 51-56, 2010
APA
Jiang, T., Zhou, L., Zhang, W., Qu, D., Xu, X., Yang, Y., & Li, S. (2010). Effects of sinomenine on proliferation and apoptosis in human lung cancer cell line NCI-H460 in vitro . Molecular Medicine Reports, 3, 51-56. https://doi.org/10.3892/mmr_00000217
MLA
Jiang, T., Zhou, L., Zhang, W., Qu, D., Xu, X., Yang, Y., Li, S."Effects of sinomenine on proliferation and apoptosis in human lung cancer cell line NCI-H460 in vitro ". Molecular Medicine Reports 3.1 (2010): 51-56.
Chicago
Jiang, T., Zhou, L., Zhang, W., Qu, D., Xu, X., Yang, Y., Li, S."Effects of sinomenine on proliferation and apoptosis in human lung cancer cell line NCI-H460 in vitro ". Molecular Medicine Reports 3, no. 1 (2010): 51-56. https://doi.org/10.3892/mmr_00000217