Inhibition by minodronate of basic fibroblast growth factor-stimulated vascular endothelial growth factor synthesis in osteoblast-like cells

  • Authors:
    • Haruhiko Tokuda
    • Seiji Adachi
    • Rie Matsushima-Nishiwaki
    • Yoshiteru Hanai
    • Shinji Takai
    • Atsushi Harada
    • Osamu Kozawa
  • View Affiliations

  • Published online on: January 1, 2010     https://doi.org/10.3892/mmr_00000235
  • Pages: 167-171
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Abstract

In our previous study, we showed that basic fibroblast growth factor (bFGF) stimulates the synthesis of vascular endothelial growth factor (VEGF) via the activation of p44/p42 mitogen-activated protein (MAP) kinase and stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) in osteoblast-like MC3T3-E1 cells. In the present study, we investigated the effect of minodronate, a newly developed bisphosphonate, on bFGF-induced VEGF synthesis in MC3T3-E1 cells. Minodronate significantly reduced the synthesis of VEGF induced by bFGF in a dose-dependent manner in a range between 3 and 100 µM. The bFGF-stimulated phosphorylation of p44/p42 MAP kinase and SAPK/JNK was reduced by minodronate. These results strongly suggest that minodronate suppresses bFGF-stimulated VEGF synthesis via the inhibition of p44/p42 MAP kinase and SAPK/JNK in osteoblasts.

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January-February 2010
Volume 3 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Tokuda H, Adachi S, Matsushima-Nishiwaki R, Hanai Y, Takai S, Harada A and Kozawa O: Inhibition by minodronate of basic fibroblast growth factor-stimulated vascular endothelial growth factor synthesis in osteoblast-like cells . Mol Med Rep 3: 167-171, 2010.
APA
Tokuda, H., Adachi, S., Matsushima-Nishiwaki, R., Hanai, Y., Takai, S., Harada, A., & Kozawa, O. (2010). Inhibition by minodronate of basic fibroblast growth factor-stimulated vascular endothelial growth factor synthesis in osteoblast-like cells . Molecular Medicine Reports, 3, 167-171. https://doi.org/10.3892/mmr_00000235
MLA
Tokuda, H., Adachi, S., Matsushima-Nishiwaki, R., Hanai, Y., Takai, S., Harada, A., Kozawa, O."Inhibition by minodronate of basic fibroblast growth factor-stimulated vascular endothelial growth factor synthesis in osteoblast-like cells ". Molecular Medicine Reports 3.1 (2010): 167-171.
Chicago
Tokuda, H., Adachi, S., Matsushima-Nishiwaki, R., Hanai, Y., Takai, S., Harada, A., Kozawa, O."Inhibition by minodronate of basic fibroblast growth factor-stimulated vascular endothelial growth factor synthesis in osteoblast-like cells ". Molecular Medicine Reports 3, no. 1 (2010): 167-171. https://doi.org/10.3892/mmr_00000235