Absence of the JAZF1/SUZ12 chimeric transcript in the immortalized non-neoplastic endometrial stromal cell line T HESCs

Panagopoulos,Ioannis

doi:10.3892/ol.2010.185

November-December 2010 Volume 1 Issue 6

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November-December 2010 Volume 1 Issue 6

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Absence of the JAZF1/SUZ12 chimeric transcript in the immortalized non-neoplastic endometrial stromal cell line T HESCs

Authors:
- Ioannis Panagopoulos
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Affiliations: Archaeological Research Laboratory, Stockholm University, Wallenberglaboratoriet, SE-106 91 Stockholm, Sweden
Pages: 947-950
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Published online on: September 23, 2010

https://doi.org/10.3892/ol.2010.185
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Abstract

Endometrial stromal sarcomas are rare malignancies, accounting for less than 10% of uterine sarcomas. The most characteristic chromosomal aberration of this tumor type is the translocation t(7;17)(p15-p21;q12-q21) leading to the fusion of two zinc finger genes, JAZF1 and SUZ12. Recently, the presence of the neoplastic JAZF1/SUZ12 fusion transcript was reported in normal cells of human endometrium. One of the positive samples for the JAZF1/SUZ12 transcript was the immortalized T HESCs cell line. This cell line was derived from the stromal cells obtained from an adult female with myomas and immortalized by transfection of a human telomerase gene. Since T HESCs has a normal karyotype and no fusion of the two genes occurs at the genomic level, the JAZF1/SUZ12 transcript was proposed to be generated by regulated trans-splicing between precursor RNAs for JAZF1 and SUZ12. However, no confirmatory reports currently exist. To determine whether the results could be reproduced, the T HESCs cell line was subjected to three different RT-PCR amplifications for the JAZF1/SUZ12 fusion transcript. RT-PCR assays did not amplify JUZF1/SUZ12 cDNA fragments in the T HESCs cell line, whereas the same assays easily generated JUZF1/SUZ12-amplified transcripts in an endometrial stromal cell sarcoma carrying the t(7;17) chromosomal aberration. Thus, the presence, if any, of a JUZF1/SUZ12 chimeric transcript in the immortalized normal T HESCs is not a constant, reproducible result.

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1	Baker P and Oliva E: Endometrial stromal tumours of the uterus: a practical approach using conventional morphology and ancillary techniques. J Clin Pathol. 60:235–243. 2007. View Article : Google Scholar : PubMed/NCBI
2	Koontz JI, Soreng AL, Nucci M, et al: Frequent fusion of the JAZF1 and JJAZ1 genes in endometrial stromal tumors. Proc Natl Acad Sci USA. 98:6348–6353. 2001. View Article : Google Scholar : PubMed/NCBI
3	Li H, Wang J, Mor G and Sklar J: A neoplastic gene fusion mimics trans-splicing of RNAs in normal human cells. Science. 321:1357–1361. 2008. View Article : Google Scholar : PubMed/NCBI
4	Krikun G, Mor G, Alvero A, et al: A novel immortalized human endometrial stromal cell line with normal progestational response. Endocrinology. 145:2291–2296. 2004. View Article : Google Scholar : PubMed/NCBI
5	Panagopoulos I, Mertens F, Domanski HA, et al: No EWS/FLI1 fusion transcripts in giant-cell tumors of bone. Int J Cancer. 93:769–772. 2001. View Article : Google Scholar : PubMed/NCBI
6	Micci F, Walter CU, Teixeira MR, et al: Cytogenetic and molecular genetic analyses of endometrial stromal sarcoma: nonrandom involvement of chromosome arms 6p and 7p and confirmation of JAZF1/JJAZ1 gene fusion in t(7;17). Cancer Genet Cytogenet. 144:119–124. 2003. View Article : Google Scholar : PubMed/NCBI
7	Brakenhoff RH, Schoenmakers JG and Lubsen NH: Chimeric cDNA clones: a novel PCR artifact. Nucleic Acids Res. 19:19491991. View Article : Google Scholar : PubMed/NCBI
8	Zeng XC and Wang SX: Evidence that BmTXK beta-BmKCT cDNA from Chinese scorpion Buthus martensii Karsch is an artifact generated in the reverse transcription process. FEBS Lett. 520:183–185. 2002. View Article : Google Scholar : PubMed/NCBI
9	Ouhammouch M and Brody EN: Temperature-dependent template switching during in vitro cDNA synthesis by the AMV-reverse transcriptase. Nucleic Acids Res. 20:5443–5450. 1992. View Article : Google Scholar : PubMed/NCBI
10	Zaphiropoulos PG: Template switching generated during reverse transcription? FEBS Lett. 527:3262002. View Article : Google Scholar : PubMed/NCBI
11	Judo MS, Wedel AB and Wilson C: Stimulation and suppression of PCR-mediated recombination. Nucleic Acids Res. 26:1819–1825. 1998. View Article : Google Scholar : PubMed/NCBI
12	Odelberg SJ, Weiss RB, Hata A and White R: Template-switching during DNA synthesis by Thermus aquaticus DNA polymerase I. Nucleic Acids Res. 23:2049–2057. 1995. View Article : Google Scholar : PubMed/NCBI
13	Shammas FV, Heikkila R and Osland A: Fluorescence-based method for measuring and determining the mechanisms of recombination in quantitative PCR. Clin Chim Acta. 304:19–28. 2001. View Article : Google Scholar : PubMed/NCBI
14	Zaphiropoulos PG: Non-homologous recombination mediated by Thermus aquaticus DNA polymerase I. Evidence supporting a copy choice mechanism. Nucleic Acids Res. 26:2843–2848. 1998.PubMed/NCBI
15	Hrzenjak A, Moinfar F, Tavassoli FA, et al: JAZF1/JJAZ1 gene fusion in endometrial stromal sarcomas: molecular analysis by reverse transcriptase-polymerase chain reaction optimized for paraffin-embedded tissue. J Mol Diagn. 7:388–395. 2005. View Article : Google Scholar

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Absence of the JAZF1/SUZ12 chimeric transcript in the immortalized non-neoplastic endometrial stromal cell line T HESCs

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