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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

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Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

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Article

Isolation and characterization of erlotinib-resistant human non-small cell lung cancer A549 cells

  • Authors:
    • Ryuji Ikeda
    • Lee C. Vermeulen
    • Elim Lau
    • Zhisheng Jiang
    • Shannon M. Kavanaugh
    • Katsushi Yamada
    • Jill M. Kolesar
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Pharmacy and Pharmacology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan, Division of Pharmacy Practice, School of Pharmacy, University of Wisconsin-Madison, Madison, and Center for Drug Policy, University of Wisconsin Hospital and Clinics, Madison, WI, USA, School of Pharmacy, University of Wisconsin-Madison, and University of Wisconsin Paul P. Carbone Comprehensive Cancer Center, Madison, WI, USA
  • Pages: 91-94
    |
    Published online on: October 19, 2010
       https://doi.org/10.3892/ol.2010.198
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Abstract

Erlotinib, an epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor, is an effective therapy for non-small cell lung cancer (NSCLC). However, resistance to erlotinib reduces its efficacy. To investigate the basis of erlotinib resistance, we isolated erlotinib-resistant human NSCLC A549 cells, termed A549/ER cells. The A549/ER cells were found to be resistant to erlotinib, as well as paclitaxel and gemcitabine. We then performed a PCR array to investigate the resistance to erlotinib in A549/ER cells. EGFR expression in A549/ER cells was decreased compared to A549 cells. The expression of fibroblast growth factor 2 (FGF2) and p21 in A549/ER was increased when compared to A549 cells. Our results suggest that the down-regulation of EGFR and up-regulation of FGF2 is related to resistance to erlotinib in A549/ER cells.
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Copy and paste a formatted citation
Spandidos Publications style
Ikeda R, Vermeulen LC, Lau E, Jiang Z, Kavanaugh SM, Yamada K and Kolesar JM: Isolation and characterization of erlotinib-resistant human non-small cell lung cancer A549 cells. Oncol Lett 2: 91-94, 2011.
APA
Ikeda, R., Vermeulen, L.C., Lau, E., Jiang, Z., Kavanaugh, S.M., Yamada, K., & Kolesar, J.M. (2011). Isolation and characterization of erlotinib-resistant human non-small cell lung cancer A549 cells. Oncology Letters, 2, 91-94. https://doi.org/10.3892/ol.2010.198
MLA
Ikeda, R., Vermeulen, L. C., Lau, E., Jiang, Z., Kavanaugh, S. M., Yamada, K., Kolesar, J. M."Isolation and characterization of erlotinib-resistant human non-small cell lung cancer A549 cells". Oncology Letters 2.1 (2011): 91-94.
Chicago
Ikeda, R., Vermeulen, L. C., Lau, E., Jiang, Z., Kavanaugh, S. M., Yamada, K., Kolesar, J. M."Isolation and characterization of erlotinib-resistant human non-small cell lung cancer A549 cells". Oncology Letters 2, no. 1 (2011): 91-94. https://doi.org/10.3892/ol.2010.198
Copy and paste a formatted citation
x
Spandidos Publications style
Ikeda R, Vermeulen LC, Lau E, Jiang Z, Kavanaugh SM, Yamada K and Kolesar JM: Isolation and characterization of erlotinib-resistant human non-small cell lung cancer A549 cells. Oncol Lett 2: 91-94, 2011.
APA
Ikeda, R., Vermeulen, L.C., Lau, E., Jiang, Z., Kavanaugh, S.M., Yamada, K., & Kolesar, J.M. (2011). Isolation and characterization of erlotinib-resistant human non-small cell lung cancer A549 cells. Oncology Letters, 2, 91-94. https://doi.org/10.3892/ol.2010.198
MLA
Ikeda, R., Vermeulen, L. C., Lau, E., Jiang, Z., Kavanaugh, S. M., Yamada, K., Kolesar, J. M."Isolation and characterization of erlotinib-resistant human non-small cell lung cancer A549 cells". Oncology Letters 2.1 (2011): 91-94.
Chicago
Ikeda, R., Vermeulen, L. C., Lau, E., Jiang, Z., Kavanaugh, S. M., Yamada, K., Kolesar, J. M."Isolation and characterization of erlotinib-resistant human non-small cell lung cancer A549 cells". Oncology Letters 2, no. 1 (2011): 91-94. https://doi.org/10.3892/ol.2010.198
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