Phenotype of tumor lymphatic vessels is a prognostic factor in human tongue squamous cell carcinoma

Nitta,Yukie; Hida,Kyoko; Kitamura,Tetsuya; Higashino,Fumihiro; Ohga,Noritaka; Fukushima,Kazuaki; Shindoh,Masanobu

doi:10.3892/ol.2010.201

January-February 2011 Volume 2 Issue 1

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

January-February 2011 Volume 2 Issue 1

Full Size Image

Article

Phenotype of tumor lymphatic vessels is a prognostic factor in human tongue squamous cell carcinoma

Authors:
- Yukie Nitta
- Kyoko Hida
- Tetsuya Kitamura
- Fumihiro Higashino
- Noritaka Ohga
- Kazuaki Fukushima
- Masanobu Shindoh
View Affiliations / Copyright

Affiliations: Department of Oral Pathology and Biology, Hokkaido University Graduate School of Dental Medicine, Sapporo 060-8586, Japan, Department of Vascular Biology, Hokkaido University Graduate School of Dental Medicine, Sapporo 060-8586, Japan, Department of Dental Anesthesiology, Hokkaido University Graduate School of Dental Medicine, Sapporo 060-8586, Japan
Pages: 79-83
|
Published online on: November 8, 2010

https://doi.org/10.3892/ol.2010.201
Expand metrics +

Abstract

Tumor metastasis to lymph nodes occurs through the lymphatic vessels located in the tumor circumference. However, few studies have focused on the phenotypes of lymphatic vessels around these tumors. We investigated the characteristics of the lymph vessels of tongue squamous cell carcinoma (SCC) and compared them to clinicopathological characteristics. A total of 43 patients diagnosed as having tongue SCC consulted Hokkaido University Hospital were examined. The lymphatic vessels were identified by antibody D2-40 and the number and diameter of tumor lymphatic vessels were measured. The proliferative activity of lymphatic endothelial cells was also examined by immunostaining using antibody MIB-1. We then measured the DNA density of lymphatic endothelial cells in normal and tumor tissues. The number of tumor lymphatic vessels significantly increased in highly metastatic cases of tongue SCC, particularly in cases with a large number of micro lymphatic vessels. A significant correlation was found between the metastatic and proliferative activity of tumor lymphatic endothelial cells. Moreover, the DNA density of tumor lymphatic endothelial cells increased compared to normal tissues. These results suggest that the phenotypes of tumor lymphatic endothelial cells are an indicator of lymph node metastasis of tongue SCC.

View Figures

View References

1	He Y, Karpanen T and Alitalo K: Role of lymphangiogenic factors in tumor metastasis. Biochim Biophys Acta. 1654:3–12. 2004.PubMed/NCBI
2	Achen MG, McColl BK and Stacker SA: Focus on lymphangiogenesis in tumor metastasis. Cancer Cell. 7:121–127. 2005. View Article : Google Scholar : PubMed/NCBI
3	Bird-Lieberman EL and Fitzgerald RC: Early diagnosis of oesophageal cancer. Br J Cancer. 101:1–6. 2009. View Article : Google Scholar
4	Karpanen T, Egeblad M, Karkkainen MJ, et al: Vascular endothelial growth factor C promotes tumor lymphangiogenesis and intralymphatic tumor growth. Cancer Res. 61:1786–1790. 2001.PubMed/NCBI
5	Nor JE and Polverini PJ: Role of endothelial cell survival and death signals in angiogenesis. Angiogenesis. 3:101–116. 1993. View Article : Google Scholar : PubMed/NCBI
6	Moldovan NI and Asahara T: Role of blood mononuclear cells in recanalization and vascularization of thrombi: past, present, and future. Trends Cardiovasc Med. 13:265–269. 2003. View Article : Google Scholar : PubMed/NCBI
7	Blagosklonny MV: Antiangiogenic therapy and tumor progression. Cancer Cell. 5:13–17. 2004. View Article : Google Scholar
8	Coultas L, Chawengsaksophak K and Rossant J: Endothelial cells and VEGF in vascular development. Nature. 438:937–945. 2005. View Article : Google Scholar : PubMed/NCBI
9	Stoeltzing O, Meric-Bernstam F and Ellis LM: Intracellular signaling in tumor and endothelial cells: the expected and, yet again, the unexpected. Cancer Cell. 10:89–91. 2006. View Article : Google Scholar : PubMed/NCBI
10	Breiteneder-Geleff S, Soleiman A, Kowalski H, et al: Angiosarcomas express mixed endothelial phenotypes of blood and lymphatic capillaries: podoplanin as a specific marker for lymphatic endothelium. Am J Pathol. 154:385–394. 1999. View Article : Google Scholar
11	Oliver G: Lymphatic vasculature development. Nat Rev Immunol. 4:35–45. 2004. View Article : Google Scholar
12	Akishima Y, Ito K, Zhang L, et al: Immunohistochemical detection of human small lymphatic vessels under normal and pathological conditions using the LYVE-1 antibody. Virchows Arch. 444:153–157. 2004. View Article : Google Scholar
13	Pepper MS and Skobe M: Lymphatic endothelium: morphological, molecular and functional properties. J Cell Biol. 163:209–213. 2003. View Article : Google Scholar : PubMed/NCBI
14	Fukunaga M: Expression of D2-40 in lymphatic endothelium of normal tissues and in vascular tumours. Histopathology. 46:396–402. 2005. View Article : Google Scholar : PubMed/NCBI
15	Koukourakis MI, Giatromanolaki A, Sivridis E, et al: LYVE-1 immunohistochemical assessment of lymphangiogenesis in endometrial and lung cancer. J Clin Pathol. 58:202–206. 2005. View Article : Google Scholar : PubMed/NCBI
16	Ueda M, Hung YC, Terai Y, et al: Vascular endothelial growth factor-C expression and invasive phenotype in ovarian carcinomas. Clin Cancer Res. 11:3225–3232. 2005. View Article : Google Scholar : PubMed/NCBI
17	Aishima S, Nishihara Y, Iguchi T, et al: Lymphatic spread is related to VEGF-C expression and D2-40-positive myofibroblasts in intrahepatic cholangiocarcinoma. Mod Pathol. 21:256–264. 2008. View Article : Google Scholar : PubMed/NCBI
18	Hagendoorn J, Padera TP, Kashiwagi S, et al: Endothelial nitric oxide synthase regulates microlymphatic flow via collecting lymphatics. Circ Res. 95:204–209. 2004. View Article : Google Scholar : PubMed/NCBI
19	Hagendoorn J, Padera TP, Fukumura D and Jain RK: Molecular regulation of microlymphatic formation and function: role of nitric oxide. Trends Cardiovasc Med. 15:169–173. 2005. View Article : Google Scholar : PubMed/NCBI
20	Padera TP, Kadambi A, di Tomaso E, et al: Lymphatic metastasis in the absence of functional intratumor lymphatics. Science. 296:1883–1886. 2002. View Article : Google Scholar : PubMed/NCBI
21	Skobe M, Hawighorst T, Jackson DG, et al: Induction of tumor lymphangiogenesis by VEGF-C promotes breast cancer metastasis. Nat Med. 7:192–198. 2001. View Article : Google Scholar : PubMed/NCBI
22	Rubio Bueno P, Naval Gias L, Garcia Delgado R, et al: Tumor DNA content as a prognostic indicator in squamous cell carcinoma of the oral cavity and tongue base. Head Neck. 20:232–239. 1998.
23	Hida K, Hida Y, Amin DN, et al: Tumor-associated endothelial cells with cytogenetic abnormalities. Cancer Res. 64:8249–8255. 2004. View Article : Google Scholar : PubMed/NCBI
24	Hida K, Hida Y and Shindoh M: Understanding tumor endothelial cell abnormalities to develop ideal anti-angiogenic therapies. Cancer Sci. 99:459–466. 2008. View Article : Google Scholar : PubMed/NCBI
25	Akino T, Hida K, Hida Y, et al: Cytogenetic abnormalities of tumor-associated endothelial cells in human malignant tumors. Am J Pathol. 175:2657–2667. 2009. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Phenotype of tumor lymphatic vessels is a prognostic factor in human tongue squamous cell carcinoma

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Related Articles