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Article

Inhibition of STAT3 reverses drug resistance acquired in temozolomide-resistant human glioma cells

  • Authors:
    • Eun-Sang Lee
    • Kyung-Kon Ko
    • Young Ae Joe
    • Seok-Gu Kang
    • Yong-Kil Hong
  • View Affiliations / Copyright

    Affiliations: Cancer Research Institute, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea, Department of Neurosurgery, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea, Department of Neurosurgery, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul 137-701, Korea
  • Pages: 115-121
    |
    Published online on: November 23, 2010
       https://doi.org/10.3892/ol.2010.210
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Abstract

The alkylating agent temozolomide (TMZ) is an effective drug used for the treatment of malignant gliomas. However, tumor relapse combined with the development of drug resistance remains a significant problem. To clarify the mechanism of the resistance of glioma cells to TMZ chemotherapy, TMZ-resistant glioma cell lines (TR cells) were generated using U373 and U251 human glioma cells, and TMZ-resistance was confirmed via viability and apoptosis assays. The TMZ-resistance of TR cells was not associated with the TMZ-resistance molecule O6-methylguanine-DNA-methyltransferase. Notably, the expression level of signal transducers and activators of transcription 3 (STAT3) and serine 727-phosphorylated STAT3 (pSTAT3-Ser727) was highly increased in TR cells, while that of 705-phosphorylated STAT3 (pSTAT3-Tyr705) was decreased. The inhibition of STAT3 expression by small interfering RNA enhanced TR cell TMZ sensitivity. These results suggest that STAT3 contributes to TMZ-resistance in gliomas and is a potential target for the reversal of TMZ-resistance in patients with a recurrent glioma.
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Copy and paste a formatted citation
Spandidos Publications style
Lee E, Ko K, Joe YA, Kang S and Hong Y: Inhibition of STAT3 reverses drug resistance acquired in temozolomide-resistant human glioma cells. Oncol Lett 2: 115-121, 2011.
APA
Lee, E., Ko, K., Joe, Y.A., Kang, S., & Hong, Y. (2011). Inhibition of STAT3 reverses drug resistance acquired in temozolomide-resistant human glioma cells. Oncology Letters, 2, 115-121. https://doi.org/10.3892/ol.2010.210
MLA
Lee, E., Ko, K., Joe, Y. A., Kang, S., Hong, Y."Inhibition of STAT3 reverses drug resistance acquired in temozolomide-resistant human glioma cells". Oncology Letters 2.1 (2011): 115-121.
Chicago
Lee, E., Ko, K., Joe, Y. A., Kang, S., Hong, Y."Inhibition of STAT3 reverses drug resistance acquired in temozolomide-resistant human glioma cells". Oncology Letters 2, no. 1 (2011): 115-121. https://doi.org/10.3892/ol.2010.210
Copy and paste a formatted citation
x
Spandidos Publications style
Lee E, Ko K, Joe YA, Kang S and Hong Y: Inhibition of STAT3 reverses drug resistance acquired in temozolomide-resistant human glioma cells. Oncol Lett 2: 115-121, 2011.
APA
Lee, E., Ko, K., Joe, Y.A., Kang, S., & Hong, Y. (2011). Inhibition of STAT3 reverses drug resistance acquired in temozolomide-resistant human glioma cells. Oncology Letters, 2, 115-121. https://doi.org/10.3892/ol.2010.210
MLA
Lee, E., Ko, K., Joe, Y. A., Kang, S., Hong, Y."Inhibition of STAT3 reverses drug resistance acquired in temozolomide-resistant human glioma cells". Oncology Letters 2.1 (2011): 115-121.
Chicago
Lee, E., Ko, K., Joe, Y. A., Kang, S., Hong, Y."Inhibition of STAT3 reverses drug resistance acquired in temozolomide-resistant human glioma cells". Oncology Letters 2, no. 1 (2011): 115-121. https://doi.org/10.3892/ol.2010.210
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