MicroRNA-124a and microRNA-34b/c are frequently methylated in all histological types of colorectal cancer and polyps, and in the adjacent normal mucosa

Deng,Guoren; Kakar,Sanjay; Kim,Young  S.

doi:10.3892/ol.2010.222

January-February 2011 Volume 2 Issue 1

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January-February 2011 Volume 2 Issue 1

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Article

MicroRNA-124a and microRNA-34b/c are frequently methylated in all histological types of colorectal cancer and polyps, and in the adjacent normal mucosa

Authors:
- Guoren Deng
- Sanjay Kakar
- Young S. Kim
View Affiliations / Copyright

Affiliations: Department of Medicine, Veterans Affairs Medical Center, University of California at San Francisco, San Francisco, CA 94121, USA, Department of Pathology, Veterans Affairs Medical Center, University of California at San Francisco, San Francisco, CA 94121, USA, Department of Medicine, Veterans Affairs Medical Center, University of California at San Francisco, San Francisco, CA 94121, USA
Pages: 175-180
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Published online on: November 23, 2010

https://doi.org/10.3892/ol.2010.222
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Abstract

MicroRNAs (miRs) are a class of small RNAs that regulate gene expression at the post-transcriptional and/or translational level by interacting with their target mRNAs. miRs are down-regulated or up-regulated in various cancer types, triggering abnormal cell differentiation, proliferation and apoptosis. miR-124a and miR-34b/c have been reported to be expressed at lower levels in colorectal cancer (CRC) due to methylation of these genes. The present study aimed to determine the methylation status of miR-124a and miR-34b/c in CRCs and polyps of various histological types, adjacent normal mucosa and ulcerative colitis. The colon cancer cell line study showed an association of the lower expression of miR-124a and miR-34b/c with the methylation of these genes and induction of the expression of these genes with the treatment by 5-aza-2'-deoxycytidine. Among nine different cancer types examined, CRC showed the highest frequency of methylation of miR-124a (cell lines 88% and tissues 99%) and miR-34b/c (cell lines 89% and tissues 93%). Mucinous and non-mucinous CRCs and all the histological types of colorectal polyps showed a high frequency of methylation of miR-124a and miR-34b/c. Notably, methylation of miR-124a (59%) and miR-34b/c (26%) was observed in the adjacent normal mucosa of CRC patients, but not in colonic mucosa from patients without cancer or with ulcerative colitis. The methylation of miR-124a in the adjacent normal mucosa was associated with the microsatellite instability of CRC, while the methylation of miR-34b/c was associated with an older age at diagnosis of CRC. The results showed that the methylation of miR-124a and miR-34b/c occured early in colorectal carcinogenesis and certain CRCs may arise from a field defect defined by the epigenetic inactivation of miRs.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

MicroRNA-124a and microRNA-34b/c are frequently methylated in all histological types of colorectal cancer and polyps, and in the adjacent normal mucosa

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