Serotonin synthesis and metabolism-related molecules in a human prostate cancer cell line

Shinka,Toshiaki; Onodera,Dai; Tanaka,Tetsuji; Shoji,Noriaki; Miyazaki,Tadaaki; Moriuchi,Tetsuya; Fukumoto,Takahiro

doi:10.3892/ol.2011.244

March-April 2011 Volume 2 Issue 2

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March-April 2011 Volume 2 Issue 2

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Article

Serotonin synthesis and metabolism-related molecules in a human prostate cancer cell line

Authors:
- Toshiaki Shinka
- Dai Onodera
- Tetsuji Tanaka
- Noriaki Shoji
- Tadaaki Miyazaki
- Tetsuya Moriuchi
- Takahiro Fukumoto
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Affiliations: Department of Urology, Wakayama Medical University, Wakayama 641-0012, Japan, Department of Oral Medicine and Surgery, Division of Oral Diagnosis, Tohoku University Graduate School of Dentistry, Aoba-ku 980-8575, Japan, Department of Obstetrics and Gynecology, Wakayama Medical University, Wakayama 641-0012, Japan, Department of Bioresources, Hokkaido University Research Center for Zoonosis Control, Sapporo 001-0020, Japan, Division of Cancer-Related Genes, Institute for Genetic Medicine, Hokkaido University, Sapporo 060-0815, Japan, Research Center for Infection-associated Cancer, Institute for Genetic Medicine, Hokkaido University, Sapporo 060-0815, Japan
Pages: 211-215
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Published online on: January 20, 2011

https://doi.org/10.3892/ol.2011.244
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Abstract

Prostate cancer is one of the most common tumors in males and its incidence is steadily increasing worldwide. Serotonin or 5-hydroxytryptamine (5-HT) is a well-known neurotransmitter that mediates a wide variety of physiological effects. An increase in the number of 5-HT-releasing neuroendocrine (NE) cells has been correlated with tumor progression. However, it is particularly unclear whether released 5-HT or the release of 5-HT has a role in tumor cell growth. We hypothesized that 5-HT synthesis and metabolism in NE cells regulate the growth of prostate cancer cells. In the present study, 5-HT was found to play a role as a cell growth factor in prostate cancer cells. Moreover, the pharmacological inhibition of 5-HT synthesis and metabolism interrupted the growth of prostate cancer cells. To confirm the existence of 5-HT in prostate cancer cells, we performed ELISA, HPLC, RT-PCR and immunohistochemical analyses. A high expression of tryptophan hydroxylase (TPH-1), dopa decarboxylase (DDC) and monoamine oxidase A (MAO-A) was noted in the prostate cancer cells when compared with normal prostate cells. Previous studies showed that 5-HT stimulated the proliferation of prostate cancer cells mediated by 5-HT receptors 5-HTR1A and R1B. However, cell proliferation was significantly inhibited when siRNA for both DDC and TPH-1 was transfected to the cells. Consequently, we propose that the secretion system of prostate NE cells capable of 5-HT synthesis and metabolism plays a significant role in prostate tumor generation and progression. These findings provide crucial clues for the development of potential pharmacotherapeutics to slow prostate tumor progression.

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1	Chin SN, Wang L, Moore M and Sridhar SS: A review of the patterns of docetaxel use for hormone-resistant prostate cancer at the Princess Margaret Hospital. Curr Oncol. 17:24–29. 2010.PubMed/NCBI
2	Sandberg AA: Endocrine control and physiology of the prostate. Prostate. 1:169–184. 1980. View Article : Google Scholar : PubMed/NCBI
3	Abrahamsson PA: Prostate cancer and active surveillance. Front Radiat Ther Oncol. 41:1–6. 2008. View Article : Google Scholar
4	Hansson J and Abrahamsson PA: Neuroendocrine differentiation in prostatic carcinoma. Scand J Urol Nephrol Suppl. 212:28–36. 2003. View Article : Google Scholar : PubMed/NCBI
5	Seuwen K and Pouyssegur J: Serotonin as a growth factor. Biochem Pharmacol. 39:958–990. 1990. View Article : Google Scholar
6	Waguespack SG, Rich T, Grubbs E, Ying AK, Perrier ND, Ayala-Ramirez M and Jimenez C: A current review of the etiology, diagnosis, and treatment of pediatric pheochromocytoma and paraganglioma. J Clin Endocrinol Metab. 95:2023–2037. 2010. View Article : Google Scholar : PubMed/NCBI
7	Rapport MM, Green AA and Page IH: Serum vasoconstrictor (serotonin) IV: Isolation and characterization. J Biol Chem. 176:1243–1251. 1948.PubMed/NCBI
8	Fukumoto T, Kema PI and Levin M: Serotonin signaling is a very early step in patterning of the left-right axis in chick and frog embryos. Curr Biol. 15:794–803. 2005. View Article : Google Scholar : PubMed/NCBI
9	Buznikov GA, Lambert HW and Lauder JM: Serotonin and serotonin-like substances as regulators of early embryogenesis and morphogenesis. Cell tissue Res. 305:177–186. 2001. View Article : Google Scholar : PubMed/NCBI
10	Marino J and Caballero J: IIoperidone for the treatment of schizophrenia. Ann Pharmacother. 44:863–870. 2010. View Article : Google Scholar : PubMed/NCBI
11	Julius D, Livelli TJ, Jessel TM and Axel R: Ectopic expression of serotonin 1c receptor and triggering of malignant transformation. Science. 244:1057–1062. 1989. View Article : Google Scholar : PubMed/NCBI
12	Siddiqui EJ, Thompson CS, Mikhailidis DP and Mumtaz FH: The role of serotonin in tumour growth (review). Oncol Rep. 14:1593–1597. 2005.PubMed/NCBI
13	Feng LR and Maguire-Zeiss KA: Gene therapy in Parkingson’s disease: rationale and current status. CNS drugs. 24:177–192. 2010.
14	Margiotti K, Wafa LA, Cheng H, Novelli G, Nelson CC and Rennie PS: Androgen-regulated genes differentially modulated by the androgen receptor coactivator L-dopa decarboxylase in human prostate cancer cells. Mol Cancer. 6:38–50. 2007. View Article : Google Scholar
15	Richard DM, Dawes MA, Mathias CW, Acheson A, Hill-Kapturczak N and Dougherty DM: L-tryptophan: basic metabolic functions, behavioral research and therapeutic indications. Int J Tryptophan Res. 2:45–60. 2009.PubMed/NCBI
16	Siddiqui EJ, Shabbir M, Mikhailidis DP, Thompson CS and Mumtaz FH: The role of serotonin (5-hydroxytryptamine 1A and 1B) receptors in prostate cancer cell proliferation. J Urol. 176:1648–1653. 2006. View Article : Google Scholar : PubMed/NCBI
17	Lembeck F: 5-Hydroxytryptamine in a carcinoid tumor. Nature. 172:910–911. 1953. View Article : Google Scholar
18	Pearse AG: The diffuse neuroendocrine system and the apud concept: related ‘endocrine’ peptides in brain, intestine, pituitary, placenta, and anuran cutaneous glands. Med Biol. 55:115–125. 1977.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Serotonin synthesis and metabolism-related molecules in a human prostate cancer cell line

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