Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
May-June 2011 Volume 2 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-June 2011 Volume 2 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Radiosensitization of human breast cancer cells to ultraviolet light by 5-fluorouracil

  • Authors:
    • Kazuhito Sasaki
    • Nelson H. Tsuno
    • Eiji Sunami
    • Kazushige Kawai
    • Yasutaka  Shuno
    • Kumiko Hongo
    • Masaya Hiyoshi
    • Manabu Kaneko
    • Koji Murono
    • Noriko Tada
    • Takako Nirei
    • Joji Kitayama
    • Koki Takahashi
    • Hirokazu Nagawa
  • View Affiliations / Copyright

    Affiliations: Department of Surgical Oncology, The University of Tokyo, Tokyo, Japan, Department of Transfusion Medicine, Faculty of Medical Sciences, The University of Tokyo, Tokyo, Japan
  • Pages: 471-476
    |
    Published online on: March 1, 2011
       https://doi.org/10.3892/ol.2011.261
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Ultraviolet light B (UVB) phototherapy is widely used to treat dermatological diseases and therefore may be a potential optional strategy in the treatment of a skin lesion infiltrated by a malignant tumor. Currently, little is known regarding the effect of UVB phototherapy on human breast cancer cells. The present study aimed to investigate the effect of UVB phototherapy, as well as the potential effect of 5-fluorouracil (5-FU), the first-line anticancer drug for breast cancer, on radiosensitizing MCF-7 human breast cancer cells, in an attempt to develop new therapeutic strategies for the treatment of locoregional recurrence of breast cancer. MCF-7 cells were incubated in the presence of 5-FU for 48 h, and UVB irradiation at 750 mJ/cm2 was administered in the midterm of 5-FU treatment. The viability of MCF-7 cells was analyzed by the trypan blue staining method. Apoptosis was quantified by flow cytometry and Hoechst 33258 staining. The cell cycle was evaluated by flow cytometry after the staining of cells with propidium iodide. The combination treatment of 5-FU and UVB resulted in a strong potentiation of the inhibitory effect of MCF-7 cell growth, dependent on the intra-S phase cell cycle arrest and induction of apoptosis, when compared to treatment with 5-FU or UVB alone. In conclusion, 5-FU sensitized human breast cancer cells to UVB phototherapy, and this combination therapy is an effective and promising strategy for the treatment of breast cancer, particularly for locoregional recurrence.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Lui H: Phototherapy of psoriasis: update with practical pearls. J Cutan Med Surg. 6:17–21. 2002. View Article : Google Scholar : PubMed/NCBI

2 

Reynolds NJ, Franklin V, Gray JC, Diffey BL and Farr PM: Narrow-band ultraviolet B and broad-band ultraviolet A phototherapy in adult atopic eczema: a randomised controlled trial. Lancet. 357:2012–2016. 2001. View Article : Google Scholar

3 

Hamzavi I, Jain H, McLean D, Shapiro J, Zeng H and Lui H: Parametric modeling of narrowband UV-B phototherapy for vitiligo using a novel quantitative tool: the Vitiligo Area Scoring Index. Arch Dermatol. 140:677–683. 2004. View Article : Google Scholar

4 

Gathers RC, Scherschun L, Malick F, Fivenson DP and Lim HW: Narrowband UVB phototherapy for early-stage mycosis fungoides. J Am Acad Dermatol. 47:191–197. 2002. View Article : Google Scholar

5 

Matsuoka Y, Yoneda K, Katsuura J, et al: Successful treatment of follicular cutaneous T-cell lymphoma without mucinosis with narrow-band UVB-irradiation. J Eur Acad Dermatol Venereol. 21:1121–1122. 2007. View Article : Google Scholar

6 

Snellman E, Klimenko T and Rantanen T: Randomized half-side comparison of narrowband UVB and trimethylpsoralen bath plus UVA treatments for psoriasis. Acta Derm Venereol. 84:132–137. 2004. View Article : Google Scholar : PubMed/NCBI

7 

Halliday GM: Inflammation, gene mutation and photoimmuno-suppression in response to UVR-induced oxidative damage contributes to photocarcinogenesis. Mutat Res. 571:107–120. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Griffiths HR, Mistry P, Herbert KE and Lunec J: Molecular and cellular effects of ultraviolet light-induced genotoxicity. Crit Rev Clin Lab Sci. 35:189–237. 1998. View Article : Google Scholar : PubMed/NCBI

9 

Cotton J and Spandau DF: Ultraviolet B-radiation dose influences the induction of apoptosis and p53 in human keratinocytes. Radiat Res. 147:148–155. 1997. View Article : Google Scholar : PubMed/NCBI

10 

Aufiero BM, Talwar H, Young C, et al: Narrow-band UVB induces apoptosis in human keratinocytes. J Photochem Photobiol B. 82:132–139. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Longley DB, Harkin DP and Johnston PG: 5-Fluorouracil: mechanisms of action and clinical strategies. Nat Rev Cancer. 3:330–338. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Huerta S, Gao X, Livingston EH, Kapur P, Sun H and Anthony T: In vitro and in vivo radiosensitization of colorectal cancer HT-29 cells by the smac mimetic JP-1201. Surgery. 148:346–353. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Dai Y, Liu M, Tang W, et al: Molecularly targeted radiosensitization of human prostate cancer by modulating inhibitor of apoptosis. Clin Cancer Res. 14:7701–7710. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Adamsen BL, Kravik L and De Angelis PM: Cellular response to chemoradiotherapy, radiotherapy and chemotherapy in two colorectal cancer cell lines. Radiat Res. 171:562–571. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Wilson GD, Bentzen SM and Harari PM: Biologic basis for combining drugs with radiation. Semin Radiat Oncol. 16:2–9. 2006. View Article : Google Scholar

16 

Clarke M, Collins R, Darby S, et al: Effects of radiotherapy and of differences in the extent of surgery for early breast cancer on local recurrence and 15-year survival: an overview of the randomised trials. Lancet. 366:2087–2106. 2005.PubMed/NCBI

17 

Shikama N, Sekiguchi K and Nakamura N: Management of locoregional recurrence of breast cancer. Breast Cancer. May 7–2010.(Epub ahead of print).

18 

Taylor ME: Breast cancer: chest wall recurrences. Curr Treat Options Oncol. 3:175–177. 2002. View Article : Google Scholar : PubMed/NCBI

19 

Holme SA and Mills CM: Crotamiton and narrow-band UVB phototherapy: novel approaches to alleviate pruritus of breast carcinoma skin infiltration. J Pain Symptom Manage. 22:803–805. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Nakayama S, Katoh EM, Tsuzuki T and Kobayashi S: Protective effect of alpha-tocopherol-6-O-phosphate against ultraviolet B-induced damage in cultured mouse skin. J Invest Dermatol. 121:406–411. 2003. View Article : Google Scholar : PubMed/NCBI

21 

Cai BX, Luo D, Lin XF and Gao J: Compound K suppresses ultraviolet radiation-induced apoptosis by inducing DNA repair in human keratinocytes. Arch Pharm Res. 31:1483–1488. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Fergin P: Photodynamic therapy of dermatoses other than non-melanoma skin cancer. Australas J Dermatol. 46(Suppl 3): S272005. View Article : Google Scholar : PubMed/NCBI

23 

Babilas P, Karrer S, Sidoroff A, Landthaler M and Szeimies RM: Photodynamic therapy in dermatology – an update. Photodermatol Photoimmunol Photomed. 21:142–149. 2005.

24 

Saad ED and Hoff PM: UFT and oral leucovorin as radiation sensitizers in rectal and other gastrointestinal malignancies. Cancer Invest. 21:624–629. 2003. View Article : Google Scholar : PubMed/NCBI

25 

Zenda S, Onozawa Y, Tahara M, et al: Feasibility study of single agent cisplatin and concurrent radiotherapy in Japanese patients with squamous cell carcinoma of the head and neck: preliminary results. Jpn J Clin Oncol. 37:725–729. 2007. View Article : Google Scholar : PubMed/NCBI

26 

Choy H, Rodriguez FF, Koester S, Hilsenbeck S and von Hoff DD: Investigation of taxol as a potential radiation sensitizer. Cancer. 71:3774–3778. 1993. View Article : Google Scholar : PubMed/NCBI

27 

Hamaoka T, Furuya Y, Yamamoto K and Kuroda Y: MCF-7 growth inhibition by ultraviolet radiation and 5-fluorouracil: the importance of treatment sequence. Cancer Lett. 154:183–187. 2000. View Article : Google Scholar : PubMed/NCBI

28 

Lawrence TS, Blackstock AW and McGinn C: The mechanism of action of radiosensitization of conventional chemotherapeutic agents. Semin Radiat Oncol. 13:13–21. 2003. View Article : Google Scholar : PubMed/NCBI

29 

Byfield J: Useful interactions between 5-fluorouracil and radiation in man: 5-fluorouracil as a radiosensitizer. CRC Press; Boca Raton, FL: 1990

30 

McKay BC, Becerril C, Spronck JC and Ljungman M: Ultraviolet light-induced apoptosis is associated with S-phase in primary human fibroblasts. DNA Repair (Amst). 1:811–820. 2002. View Article : Google Scholar : PubMed/NCBI

31 

Sasaki K, Tsuno NH, Sunami E, et al: Chloroquine potentiates the anti-cancer effect of 5-fluorouracil on colon cancer cells. BMC Cancer. 10:3702010. View Article : Google Scholar : PubMed/NCBI

32 

Lawrence TS, Davis MA and Loney TL: Fluoropyrimidine-mediated radiosensitization depends on cyclin E-dependent kinase activation. Cancer Res. 56:3203–3206. 1996.PubMed/NCBI

33 

Naida JD, Davis MA and Lawrence TS: The effect of activation of wild-type p53 function on fluoropyrimidine-mediated radiosensitization. Int J Radiat Oncol Biol Phys. 41:675–680. 1998. View Article : Google Scholar : PubMed/NCBI

34 

Lawrence TS, Davis MA, Tang HY and Maybaum J: Fluoro-deoxyuridine-mediated cytotoxicity and radiosensitization require S phase progression. Int J Radiat Biol. 70:273–280. 1996. View Article : Google Scholar : PubMed/NCBI

35 

Panno ML, Giordano F, Mastroianni F, et al: Breast cancer cell survival signal is affected by bergapten combined with an ultraviolet irradiation. FEBS Lett. 584:2321–2326. 2010. View Article : Google Scholar : PubMed/NCBI

36 

Fraile RJ, Baker LH, Buroker TR, Horwitz J and Vaitkevicius VK: Pharmacokinetics of 5-fluorouracil administered orally, by rapid intravenous and by slow infusion. Cancer Res. 40:2223–2228. 1980.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Sasaki K, Tsuno NH, Sunami E, Kawai K, Shuno Y, Hongo K, Hiyoshi M, Kaneko M, Murono K, Tada N, Tada N, et al: Radiosensitization of human breast cancer cells to ultraviolet light by 5-fluorouracil. Oncol Lett 2: 471-476, 2011.
APA
Sasaki, K., Tsuno, N.H., Sunami, E., Kawai, K., Shuno, Y., Hongo, K. ... Nagawa, H. (2011). Radiosensitization of human breast cancer cells to ultraviolet light by 5-fluorouracil. Oncology Letters, 2, 471-476. https://doi.org/10.3892/ol.2011.261
MLA
Sasaki, K., Tsuno, N. H., Sunami, E., Kawai, K., Shuno, Y., Hongo, K., Hiyoshi, M., Kaneko, M., Murono, K., Tada, N., Nirei, T., Kitayama, J., Takahashi, K., Nagawa, H."Radiosensitization of human breast cancer cells to ultraviolet light by 5-fluorouracil". Oncology Letters 2.3 (2011): 471-476.
Chicago
Sasaki, K., Tsuno, N. H., Sunami, E., Kawai, K., Shuno, Y., Hongo, K., Hiyoshi, M., Kaneko, M., Murono, K., Tada, N., Nirei, T., Kitayama, J., Takahashi, K., Nagawa, H."Radiosensitization of human breast cancer cells to ultraviolet light by 5-fluorouracil". Oncology Letters 2, no. 3 (2011): 471-476. https://doi.org/10.3892/ol.2011.261
Copy and paste a formatted citation
x
Spandidos Publications style
Sasaki K, Tsuno NH, Sunami E, Kawai K, Shuno Y, Hongo K, Hiyoshi M, Kaneko M, Murono K, Tada N, Tada N, et al: Radiosensitization of human breast cancer cells to ultraviolet light by 5-fluorouracil. Oncol Lett 2: 471-476, 2011.
APA
Sasaki, K., Tsuno, N.H., Sunami, E., Kawai, K., Shuno, Y., Hongo, K. ... Nagawa, H. (2011). Radiosensitization of human breast cancer cells to ultraviolet light by 5-fluorouracil. Oncology Letters, 2, 471-476. https://doi.org/10.3892/ol.2011.261
MLA
Sasaki, K., Tsuno, N. H., Sunami, E., Kawai, K., Shuno, Y., Hongo, K., Hiyoshi, M., Kaneko, M., Murono, K., Tada, N., Nirei, T., Kitayama, J., Takahashi, K., Nagawa, H."Radiosensitization of human breast cancer cells to ultraviolet light by 5-fluorouracil". Oncology Letters 2.3 (2011): 471-476.
Chicago
Sasaki, K., Tsuno, N. H., Sunami, E., Kawai, K., Shuno, Y., Hongo, K., Hiyoshi, M., Kaneko, M., Murono, K., Tada, N., Nirei, T., Kitayama, J., Takahashi, K., Nagawa, H."Radiosensitization of human breast cancer cells to ultraviolet light by 5-fluorouracil". Oncology Letters 2, no. 3 (2011): 471-476. https://doi.org/10.3892/ol.2011.261
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team