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Article

Development and applications of a nasopharyngeal carcinoma Tet-Off cell line

  • Authors:
    • Yu-Peng Feng
    • Qi-Cai Liu
    • Jun-Feng Zhu
    • Fu-Kang Xie
    • Li Li
  • View Affiliations / Copyright

    Affiliations: Department of Histology and Embryology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, P.R. China, Experimental Medical Research Center, Guangzhou Medical College, Guangzhou 510182, P.R. China, Department of Pathology, The First Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510060, P.R. China, Imaging Diagnosis and Interventional Center, Cancer Center, Sun Yat-Sen University, Guangzhou 510060, P.R. China
  • Pages: 525-530
    |
    Published online on: March 1, 2011
       https://doi.org/10.3892/ol.2011.262
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Abstract

The conditional activation and inactivation of target gene expression in a nasopharyngeal carcinoma (NPC) cell line is beneficial for the study of the roles of NPC-related genes. Based on the Tet-Off Advanced system, a NPC S18 Tet-Off cell line was developed by stable transfection of a pTet-Off Advanced vector (regulator plasmid in Tet-Off Advanced system) into NPC S18 cells. Doxycycline-dependent regulators expressed in the S18 Tet-Off cells were examined by transient and stable transfection of pTRE-Tight-Luc. The S18 Tet-Off-Luc clone selected by stable transfection of pTRE-Tight-Luc into S18 Tet-Off cells expressed firefly luciferase under tight control of doxycycline in a time- and dose-dependent manner. To test applications of the S18 Tet-Off cell line in the study of gene function, the impact of ferritin heavy chain (FTH1) gene on NPC cell growth was examined. The S18 Tet-Off-FTH1 clone was developed by stably transfecting pTRE-Tight-FTH1 (response plasmid harboring FTH1) into S18 Tet-Off cells. FTH1 levels in the S18 Tet-Off-FTH1 clone were semi-quantitatively regulated in response to varying concentrations of doxycycline. A cell proliferation assay showed that a high expression of FTH1 (cells grown in the absence of doxycycline) reduced cell growth, while moderate FTH1 overexpression (cells grown in 0.1 ng/ml doxycycline) had no adverse effect on cell growth. In conclusion, the S18 Tet-Off cell line provides a proven genetic background for convenient access to controllable gene expression in NPC.
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1 

Wei WI and Sham JS: Nasopharyngeal carcinoma. Lancet. 365:2041–2054. 2005. View Article : Google Scholar : PubMed/NCBI

2 

Lu H, Peng LX, Yuan XB, et al: Concurrent chemoradiotherapy in locally advanced nasopharyngeal carcinoma: a treatment paradigm also applicable to patients in Southeast Asia. Cancer Treat Rev. 35:345–353. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Agulnik M and Epstein JB: Nasopharyngeal carcinoma: current management, future directions and dental implications. Oral Oncol. 44:617–627. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Qian CN, Berghuis B, Tsarfaty G, et al: Preparing the ‘soil’: the primary tumor induces vasculature reorganization in the sentinel lymph node before the arrival of metastatic cancer cells. Cancer Res. 66:10365–10376. 2006.

5 

Gossen M and Bujard H: Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci USA. 89:5547–5551. 1992. View Article : Google Scholar : PubMed/NCBI

6 

Urlinger S, Baron U, Thellmann M, Hasan MT, Bujard H and Hillen W: Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity. Proc Natl Acad Sci USA. 97:7963–7968. 2000. View Article : Google Scholar

7 

Gossen M and Bujard H: Efficacy of tetracycline-controlled gene expression is influenced by cell type: commentary. Biotechniques. 19:213–217. 1995.PubMed/NCBI

8 

Ackland-Berglund CE and Leib DA: Efficacy of tetracycline- controlled gene expression is influenced by cell type. Biotechniques. 18:196–200. 1995.PubMed/NCBI

9 

Howe JR, Skryabin BV, Belcher SM, Zerillo CA and Schmauss C: The responsiveness of a tetracycline-sensitive expression system differs in different cell lines. J Biol Chem. 270:14168–14174. 1995. View Article : Google Scholar : PubMed/NCBI

10 

Marks JE, Phillips JL and Menck HR: The National Cancer Data Base report on the relationship of race and national origin to the histology of nasopharyngeal carcinoma. Cancer. 83:582–588. 1998. View Article : Google Scholar : PubMed/NCBI

11 

Reddy SP, Raslan WF, Gooneratne S, Kathuria S and Marks JE: Prognostic significance of keratinization in nasopharyngeal carcinoma. Am J Otolaryngol. 16:103–108. 1995. View Article : Google Scholar : PubMed/NCBI

12 

Wang XS, Hu CS, Ying HM, Zhou ZR, Ding JH and Feng Y: Patterns of retropharyngeal node metastasis in nasopharyngeal carcinoma. Int J Radiat Oncol Biol Phys. 73:194–201. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Freundlieb S, Schirra-Muller C and Bujard H: A tetracycline controlled activation/repression system with increased potential for gene transfer into mammalian cells. J Gene Med. 1:4–12. 1999. View Article : Google Scholar : PubMed/NCBI

14 

Rennel E and Gerwins P: How to make tetracycline-regulated transgene expression go on and off. Anal Biochem. 309:79–84. 2002. View Article : Google Scholar : PubMed/NCBI

15 

Cohen B, Ziv K, Plaks V, et al: MRI detection of transcriptional regulation of gene expression in transgenic mice. Nat Med. 13:498–503. 2007. View Article : Google Scholar : PubMed/NCBI

16 

Genove G, DeMarco U, Xu H, Goins WF and Ahrens ET: A new transgene reporter for in vivo magnetic resonance imaging. Nat Med. 11:450–454. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Zurkiya O, Chan AWS and Hu XP: MagA is sufficient for producing magnetic nanoparticles in mammalian cells, making it an MRI reporter. Magn Reson Med. 59:1225–1231. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Cozzi A, Corsi B, Levi S, Santambrogio P, Albertini A and Arosio P: Overexpression of wild type and mutated human ferritin H-chain in HeLa cells: in vivo role of ferritin ferroxidase activity. J Biol Chem. 275:25122–25129. 2000. View Article : Google Scholar : PubMed/NCBI

19 

Cohen B, Dafni H, Meir G, Harmelin A and Neeman M: Ferritin as an endogenous MRI reporter for noninvasive imaging of gene expression in C6 glioma tumors. Neoplasia. 7:109–117. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Naumova AV, Reinecke H, Yarnykh V, Deem J, Yuan C and Murry CE: Ferritin overexpression for noninvasive magnetic resonance imaging-based tracking of stem cells transplanted into the heart. Mol Imaging. 9:201–210. 2010.PubMed/NCBI

21 

Liu J, Cheng ECH, Long RC, et al: Noninvasive monitoring of embryonic stem cells in vivo with MRI transgene reporter. Tissue Engineering Part C Methods. 15:739–747. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Feng Y, Liu Q, Zhu J, Xie F and Li L: Development and applications of a nasopharyngeal carcinoma Tet-Off cell line. Oncol Lett 2: 525-530, 2011.
APA
Feng, Y., Liu, Q., Zhu, J., Xie, F., & Li, L. (2011). Development and applications of a nasopharyngeal carcinoma Tet-Off cell line. Oncology Letters, 2, 525-530. https://doi.org/10.3892/ol.2011.262
MLA
Feng, Y., Liu, Q., Zhu, J., Xie, F., Li, L."Development and applications of a nasopharyngeal carcinoma Tet-Off cell line". Oncology Letters 2.3 (2011): 525-530.
Chicago
Feng, Y., Liu, Q., Zhu, J., Xie, F., Li, L."Development and applications of a nasopharyngeal carcinoma Tet-Off cell line". Oncology Letters 2, no. 3 (2011): 525-530. https://doi.org/10.3892/ol.2011.262
Copy and paste a formatted citation
x
Spandidos Publications style
Feng Y, Liu Q, Zhu J, Xie F and Li L: Development and applications of a nasopharyngeal carcinoma Tet-Off cell line. Oncol Lett 2: 525-530, 2011.
APA
Feng, Y., Liu, Q., Zhu, J., Xie, F., & Li, L. (2011). Development and applications of a nasopharyngeal carcinoma Tet-Off cell line. Oncology Letters, 2, 525-530. https://doi.org/10.3892/ol.2011.262
MLA
Feng, Y., Liu, Q., Zhu, J., Xie, F., Li, L."Development and applications of a nasopharyngeal carcinoma Tet-Off cell line". Oncology Letters 2.3 (2011): 525-530.
Chicago
Feng, Y., Liu, Q., Zhu, J., Xie, F., Li, L."Development and applications of a nasopharyngeal carcinoma Tet-Off cell line". Oncology Letters 2, no. 3 (2011): 525-530. https://doi.org/10.3892/ol.2011.262
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