Neurotransmitter γ-aminobutyric acid-mediated inhibition of the invasive ability of cholangiocarcinoma cells

  • Authors:
    • Qiang Huang
    • Chenhai Liu
    • Cheng Wang
    • Yuanguo Hu
    • Lujun Qiu
    • Peng Xu
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  • Published online on: March 4, 2011     https://doi.org/10.3892/ol.2011.263
  • Pages: 519-523
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Abstract

The present study aimed to investigate the effect and mechanism of γ-aminobutyric acid (GABA) on the bio-nomics of cholangiocarcinoma QBC939 cells. A transwell cell culture chamber assay in vitro was used to detect the ability of invasion and metastasis of cholangiocarcinoma QBC939 cells. The expression of matrix metalloproteinase (MMP) mRNA and the enzymatic activity in QBC939 cells were detected by reverse transcription-polymerase chain reaction and gelatin zymography, respectively. Different concentrations of GABA inhibited the ability of the tumor cells in trans-Matrigel migration (100 µmol/l GABA, 60±9.54 vs. 42.67±4.04, P<0.05), and inhibited the activity and expression of MMP-2 and MMP-9. GABA inhibited the invasion and metastasis of cholangiocarcinoma QBC939 cells, and the mechanism may involve the down-regulation of the activity of MMPs.
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May-June 2011
Volume 2 Issue 3

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Online ISSN:1792-1082

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Spandidos Publications style
Huang Q, Liu C, Wang C, Hu Y, Qiu L and Xu P: Neurotransmitter γ-aminobutyric acid-mediated inhibition of the invasive ability of cholangiocarcinoma cells. Oncol Lett 2: 519-523, 2011
APA
Huang, Q., Liu, C., Wang, C., Hu, Y., Qiu, L., & Xu, P. (2011). Neurotransmitter γ-aminobutyric acid-mediated inhibition of the invasive ability of cholangiocarcinoma cells. Oncology Letters, 2, 519-523. https://doi.org/10.3892/ol.2011.263
MLA
Huang, Q., Liu, C., Wang, C., Hu, Y., Qiu, L., Xu, P."Neurotransmitter γ-aminobutyric acid-mediated inhibition of the invasive ability of cholangiocarcinoma cells". Oncology Letters 2.3 (2011): 519-523.
Chicago
Huang, Q., Liu, C., Wang, C., Hu, Y., Qiu, L., Xu, P."Neurotransmitter γ-aminobutyric acid-mediated inhibition of the invasive ability of cholangiocarcinoma cells". Oncology Letters 2, no. 3 (2011): 519-523. https://doi.org/10.3892/ol.2011.263