Neurotransmitter γ-aminobutyric acid-mediated inhibition of the invasive ability of cholangiocarcinoma cells

Huang,Qiang; Liu,Chenhai; Wang,Cheng; Hu,Yuanguo; Qiu,Lujun; Xu,Peng

doi:10.3892/ol.2011.263

May-June 2011 Volume 2 Issue 3

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

May-June 2011 Volume 2 Issue 3

Full Size Image

Article

Neurotransmitter γ-aminobutyric acid-mediated inhibition of the invasive ability of cholangiocarcinoma cells

Authors:
- Qiang Huang
- Chenhai Liu
- Cheng Wang
- Yuanguo Hu
- Lujun Qiu
- Peng Xu
View Affiliations / Copyright

Affiliations: Department of General Surgery, Affiliated Provincial Hospital of Anhui Medical University, Hefei 230001, P.R. China
Pages: 519-523
|
Published online on: March 4, 2011

https://doi.org/10.3892/ol.2011.263
Expand metrics +

Abstract

The present study aimed to investigate the effect and mechanism of γ-aminobutyric acid (GABA) on the bio-nomics of cholangiocarcinoma QBC939 cells. A transwell cell culture chamber assay in vitro was used to detect the ability of invasion and metastasis of cholangiocarcinoma QBC939 cells. The expression of matrix metalloproteinase (MMP) mRNA and the enzymatic activity in QBC939 cells were detected by reverse transcription-polymerase chain reaction and gelatin zymography, respectively. Different concentrations of GABA inhibited the ability of the tumor cells in trans-Matrigel migration (100 µmol/l GABA, 60±9.54 vs. 42.67±4.04, P<0.05), and inhibited the activity and expression of MMP-2 and MMP-9. GABA inhibited the invasion and metastasis of cholangiocarcinoma QBC939 cells, and the mechanism may involve the down-regulation of the activity of MMPs.

View Figures

View References

1	Entschladen F, Lang K, Drell TL, Joseph J and Zanker KS: Neurotransmitters are regulators for the migration of tumor cells and leukocytes. Cancer Immunol Immunother. 51:467–482. 2002. View Article : Google Scholar : PubMed/NCBI
2	Li L, Gang C and Zhang GY: Effects of neurotransmitters on the proliferation of human hepatocytes. Chin J Bases Clin General Surg. 9:238–241. 2002.
3	Wu Fy, Ou ZL and Shao ZM: Classical neurotransmitter and cancer metastasis. Foreign Medical Sciences (Oncology section). 32:678–680. 2005.
4	Watanabe M, Maemura K, Kanbara K, Tamayama T and Hayasaki H: GABA and GABA receptors in the central nervous system and other organs. Int Rev Cytol. 213:1–47. 2002. View Article : Google Scholar : PubMed/NCBI
5	Roberts E: Adventures with GABA: fifty years on. GABA in the Nervous System. The View at Fifty Years. Martin D and Olsen R: Lippincott Williams and Wilkins; Philadelphia: pp. 1–24. 2000
6	Saito N, Taniyama K and Tanaka C: Uptake and release of gamma-aminobutyric acid in guinea pig gallbladder. Am J Physiol Gastrointest Liver Physiol. 249:G192–G196. 1985.PubMed/NCBI
7	Maemura K, Yamauchi H, Hayasaki H, et al: Gamma-amino-butyric acid immunoreactivity in intramucosal colonic tumors. J Gastroenterol Hepatol. 18:1089–1094. 2003. View Article : Google Scholar : PubMed/NCBI
8	Kleinrok Z, Matuszek M, Jesipowicz J, Matuszek B, Opolski A and Radzikowski C: GABA content and GAD activity in colon tumors taken from patients with colon cancer or from xenografted human colon cancer cells growing as s. c tumors in athymic nu/nu mice. J Physiol Pharmacol. 49:303–310. 1998.PubMed/NCBI
9	Matuszek M, Jesipowicz M and Kleinrok Z: GABA content and GAD activity in gastric cancer. Med Sci Monit. 7:377–381. 2001.PubMed/NCBI
10	Ortega A: A new role for GABA: inhibition of tumor cell migration. Trends Pharmacol Sci. 24:151–154. 2003. View Article : Google Scholar : PubMed/NCBI
11	Azuma H, Inamoto T, Sakamoto T, et al: Gamma-aminobutyric acid as a promoting factor of cancer metastasis; induction of matrix metalloproteinase production is potentially its underlying mechanism. Cancer Res. 63:8090–8096. 2003.
12	Joseph J, Niggemarm B, Zaenker KS, et al: The neurotransmitter gamma-aminobutyric acid is an inhibitory regulator for the migration of SW 480 colon carcinoma cells. Cancer Res. 62:6467–6469. 2002.PubMed/NCBI
13	Liu CH, Huang Q and Wang C: Gamma-aminobutyric acid-induced apoptosis and effect of telomerase activity of cholangiocarcinoma cell line QBC939. Chin J Hepatobiliary Surg. 15:41–44. 2009.
14	Wang SG, Han BL, Duan HC, et al: Establishment of the characteristics of a cell line of extrahepatic cholangiocarcinoma. Chin J Exp Surg. 14:67–70. 1997.
15	Ries C, Loher F, Zang C, Ismair MG and Petrides PE: Matrix metalloproteinase production by bone marrow mononuclear cells from normal individuals and patients with acute and chronic myeloid leukemia or myelodysplastic syndromes. Clin Cancer Res. 5:1115–1124. 1999.
16	Stetler-Stevenson WG and Kleiner: Molecular biology of cancer: invasion and metastases. Cancer Principles and Practice of Oncology. 6th edition. Devita VT, Hellman S and Rosenberg SA: Lippincott Williams Willkins; Philadelphia: pp. 123–136. 2001
17	Hanahan D and Weinberg RA: The hallmarks of cancer. Cell. 100:57–70. 2000. View Article : Google Scholar
18	Chanda R and Ganguly AK: Diamine-oxidase activity and tissue di- and poly-amine contents of human ovarian, cervical and endometrial carcinoma. Cancer Lett. 89:23–28. 1995. View Article : Google Scholar : PubMed/NCBI
19	Nicholson-Guthrie CS, Guthrie GD, Sutton GP and Baenziger JC: Urine GABA levels in ovarian cancer patients: elevated GABA in malignancy. Cancer Lett. 162:27–30. 2001. View Article : Google Scholar : PubMed/NCBI
20	Coussens LM, Fingleton B and Matrisian LM: Matrix metalloproteinase inhibitors and cancer: trials and tribulations. Science. 295:2387–2392. 2002. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Neurotransmitter γ-aminobutyric acid-mediated inhibition of the invasive ability of cholangiocarcinoma cells

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Related Articles