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Article

Induction of apoptosis in the SW620 colon carcinoma cell line by triterpene-enriched extracts from Ganoderma lucidum through activation of caspase-3

  • Authors:
    • Zhe Ji
    • Qingjiu Tang
    • Ruixia Hao
    • Jinsong Zhang
    • Yingjie Pan
  • View Affiliations / Copyright

    Affiliations: Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201106, P.R. China, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201106, P.R. China, Shanghai Fisheries University, Shanghai 200090, P.R. China
  • Pages: 565-570
    |
    Published online on: March 21, 2011
       https://doi.org/10.3892/ol.2011.275
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Abstract

The medicinal mushroom Ganoderma lucidum (G. lucidum) has been used for the treatment of various diseases, and is known for the immune-enhancing activity of its polysaccharide. However, little is known about another of its major constituents, triterpene. This study investigated the anticancer mechanism of a triterpene-enriched extract from G. lucidum. The triterpene-enriched extract, GLAI, was prepared from fruiting bodies of G. lucidum by sequential hot water extraction, removal of ethanol-insoluble polysaccharides and gel-filtration chromatography. The mechanisms of GLAI-induced apoptosis on SW620 human colorectal adenocarcinoma cells were investigated. Tumor cell lines in vitro were treated with different concentrations of GLAI. Cell proliferation was measured by the Alamar blue assay, morphology of cell apoptosis was observed, cell apoptosis was detected by flow cytometry (FCM) and caspase-3 activity was detected by Caspase-3 cellular activity assay. The results showed that GLAI inhibited the growth of different tumor cells and caused significant apoptosis in a dose-dependent manner. Marked morphological changes of cell apoptosis were observed after the cells had been exposed to GLAI for 24 h. The Caspase-3 assay results showed that the activity of the caspase-3 enzyme increased in both a time- and dose-dependent manner, whereas GLAI resulted in the down-regulation of Bcl-2 gene expression at the mRNA level and XIAP protein production at the protein level. Conversely, GLAI up-regulates the expression of the apoptosis enhancer Bax gene and p53 protein. These findings suggest that the triterpenes contained in G. lucidum are potential anticancer agents.
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1 

Wasson RG: Soma-Divine Mushroom of Immortality. Harcourt, Brace & World; New York: pp. 3811968

2 

Chang ST and Buswell JA: Ganoderma lucidum (Curt.:Fr) P. Karst. (Aphyllophoromycetideae) – a mushrooming medicinal mushroom. International Journal of Medicinal Mushrooms. 1:139–146. 1999. View Article : Google Scholar

3 

Wachtel-Galor S, Benzie IFF, Tomlinson B and Buswell JA: Lingzhi (Ganoderma lucidum): molecular aspects of health effects. Herbal Medicines. Packer L, Halliwell B and Ong CN: Marcel Dekker Inc; New York: pp. 179–228. 2004

4 

Furusawa E, Chou SC, Furusawa S, Hirazumi A and Dang Y: Antitumor activity of Ganoderma lucidum, an edible mushroom, on intraperitoneal implanted Lewis lung carcinoma in syngeneic mice. Phytotherapy Research. 6:300–304. 1992.

5 

Lieu CW, Lee SS and Wang SY: The effect of Ganoderma lucidum on induction of differentiation in leukemic U937 cells. Anticancer Res. 12:1211–1215. 1992.

6 

Wang SY, Hsu ML, Hsu HC, et al: The anti-tumor effect of Ganoderma lucidum is mediated by cytokines released from activated macrophages and T lymphocytes. Int J Cancer. 70:699–705. 1997.

7 

Zhu M, Chang Q, Wong LK, Chong FS and Li RC: Triterpene antioxidants from ganoderma lucidum. Phytother Res. 13:529–531. 1999. View Article : Google Scholar

8 

Kim DH, Shim SB, Kim NJ and Jang IS: Beta-glucuronidase-inhibitory activity and hepatoprotective effect of Ganoderma lucidum. Biol Pharm Bull. 22:162–164. 1999. View Article : Google Scholar : PubMed/NCBI

9 

Komoda Y, Shimizu M, Sonoda Y and Sato Y: Ganoderic acid and its derivatives as cholesterol synthesis inhibitors. Chem Pharma Bull. 37:531–533. 1989. View Article : Google Scholar : PubMed/NCBI

10 

Kabir Y, Kimura S and Tamura T: Dietary effect of Ganoderma lucidum mushroom on blood pressure and lipid levels in spontaneously hypertensive rats (SHR). J Nutr Sci Vitaminol. 34:433–438. 1988.

11 

Lee SY and Rhee HM: Cardiovascular effects of mycelium extract of Ganoderma lucidum: inhibition of sympathetic outflow as a mechanism of its hypotensive action. Chem Pharma Bull. 38:1359–1364. 1990.PubMed/NCBI

12 

Su CY, Shiao MS and Wang CT: Differential effects of ganodermic acid S on the thromboxane A2-signaling pathways in human platelets. Biochem Pharmacol. 58:587–595. 1999. View Article : Google Scholar : PubMed/NCBI

13 

Gan KH, Fann YF, Hsu SH, Kuo KW and Lin CN: Mediation of the cytotoxicity of lanostanoids and steroids of Ganoderma tsugae through apoptosis and cell cycle. J Nat Prod. 61:485–487. 1998. View Article : Google Scholar : PubMed/NCBI

14 

Min BS, Gao JJ, Nakamura N and Hattori M: Triterpenes from the spores of Ganoderma lucidum and their cytotoxicity against meth-A and LLC tumor cells. Chem Pharma Bull. 48:1026–1033. 2000.PubMed/NCBI

15 

Noda Y, Kaiya T, Kohda K and Kawazoe Y: Enhanced cytotoxicity of some triterpenes toward leukemia L1210 cells cultured in low pH media: possibility of a new mode of cell killing. Chem Pharma Bull. 45:1665–1670. 1997. View Article : Google Scholar : PubMed/NCBI

16 

Wu TS, Shi LS and Kuo SC: Cytotoxicity of Ganoderma lucidum triterpenes. J Nat Prod. 64:1121–1122. 2001.

17 

Beauparlant P and Shore GC: Therapeutic activation of caspases in cancer: a question of selectivity. Curr Opin Drug Discov Devel. 6:179–187. 2003.PubMed/NCBI

18 

Thorburn A: Death receptor-induced cell killing. Cell Signal. 16:139–144. 2004. View Article : Google Scholar

19 

Qi SN, Yoshida A, Wang ZR and Ueda T: GP7 can induce apoptotic DNA fragmentation of human leukemia cells through caspase-3-dependent and -independent pathways. Int J Mol Med. 13:163–167. 2004.PubMed/NCBI

20 

Zhang JF, Liu JJ, Liu PQ, Lin DJ, Li XD and Chen GH: Oridonin inhibits cell growth by induction of apoptosis on human hepatocelluar carcinoma BEL-7402 cells. Hepatol Res. 35:104–110. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Thornberry NA and Lazebnik Y: Caspases: enemies within. Science. 281:1312–1316. 1998. View Article : Google Scholar : PubMed/NCBI

22 

Subramaniam V, Vincent IR and Jothy S: Upregulation and dephosphorylation of cofilin: modulation by CD44 variant isoform in human colon cancer cells. Exp Mol Pathol. 79:187–193. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Martin SJ, Reutelingsperger CP, McGahon AJ, et al: Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl. J Exp Med. 182:1545–1556. 1995. View Article : Google Scholar

24 

Donepudi M and Grutter MG: Structure and zymogen activation of caspases. Biophys Chem. 101–102:145–153. 2002.PubMed/NCBI

25 

Denault JB and Salvesen GS: Caspases: keys in the ignition of cell death. Chem Rev. 102:4489–4500. 2002. View Article : Google Scholar : PubMed/NCBI

26 

Boatright KM and Salvesen GS: Caspase activation. Biochem Soc Symp. 233–242. 2003.

27 

Philchenkov AA: Caspases as regulators of apoptosis and other cell functions. Biochemistry. 68:365–376. 2003.PubMed/NCBI

28 

Huether A, Hopfner M, Sutter AP, Schuppan D and Scherubl H: Erlotinib induces cell cycle arrest and apoptosis in hepatocellular cancer cells and enhances chemosensitivity towards cytostatics. J Hepatol. 43:661–669. 2005. View Article : Google Scholar : PubMed/NCBI

29 

Nagata S: Apoptosis by death factor. Cell. 88:355–365. 1997. View Article : Google Scholar : PubMed/NCBI

30 

Srinivasan A, Roth KA, Sayers RO, et al: In situ immunodetection of activated caspase-3 in apoptotic neurons in the developing nervous system. Cell Death Differ. 5:1004–1016. 1998. View Article : Google Scholar : PubMed/NCBI

31 

Tanabe H, Eguchi Y, Shimizu S, Martinou JC and Tsujimoto Y: Death-signalling cascade in mouse cerebellar granule neurons. Eur J Neurosci. 10:1403–1411. 1998. View Article : Google Scholar : PubMed/NCBI

32 

Bhathena SJ and Velasquez MT: Beneficial role of dietary phytoestrogens in obesity and diabetes. Am J Clin Nutr. 76:1191–1201. 2002.PubMed/NCBI

33 

Gross A, McDonnell JM and Korsmeyer SJ: BCL-2 family members and the mitochondria in apoptosis. Genes Dev. 13:1899–1911. 1999. View Article : Google Scholar : PubMed/NCBI

34 

Oltvai ZN, Milliman CL and Korsmeyer SJ: Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell. 74:609–619. 1993. View Article : Google Scholar : PubMed/NCBI

35 

Choi YH, Kong KR, Kim YA, et al: Induction of Bax and activation of caspases during β-sitosterol-mediated apoptosis in human colon cancer cells. Int J Oncol. 23:1657–1662. 2003.

36 

Levy P, Robin H, Bertrand F, Kornprobst M and Capeau J: Butyrate-treated colonic Caco-2 cells exhibit defective integrin-mediated signaling together with increased apoptosis and differentiation. J Cell Physiol. 197:336–347. 2003. View Article : Google Scholar

37 

White E: Life, death, and the pursuit of apoptosis. Genes Dev. 10:1–15. 1996. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Ji Z, Tang Q, Hao R, Zhang J and Pan Y: Induction of apoptosis in the SW620 colon carcinoma cell line by triterpene-enriched extracts from Ganoderma lucidum through activation of caspase-3. Oncol Lett 2: 565-570, 2011.
APA
Ji, Z., Tang, Q., Hao, R., Zhang, J., & Pan, Y. (2011). Induction of apoptosis in the SW620 colon carcinoma cell line by triterpene-enriched extracts from Ganoderma lucidum through activation of caspase-3. Oncology Letters, 2, 565-570. https://doi.org/10.3892/ol.2011.275
MLA
Ji, Z., Tang, Q., Hao, R., Zhang, J., Pan, Y."Induction of apoptosis in the SW620 colon carcinoma cell line by triterpene-enriched extracts from Ganoderma lucidum through activation of caspase-3". Oncology Letters 2.3 (2011): 565-570.
Chicago
Ji, Z., Tang, Q., Hao, R., Zhang, J., Pan, Y."Induction of apoptosis in the SW620 colon carcinoma cell line by triterpene-enriched extracts from Ganoderma lucidum through activation of caspase-3". Oncology Letters 2, no. 3 (2011): 565-570. https://doi.org/10.3892/ol.2011.275
Copy and paste a formatted citation
x
Spandidos Publications style
Ji Z, Tang Q, Hao R, Zhang J and Pan Y: Induction of apoptosis in the SW620 colon carcinoma cell line by triterpene-enriched extracts from Ganoderma lucidum through activation of caspase-3. Oncol Lett 2: 565-570, 2011.
APA
Ji, Z., Tang, Q., Hao, R., Zhang, J., & Pan, Y. (2011). Induction of apoptosis in the SW620 colon carcinoma cell line by triterpene-enriched extracts from Ganoderma lucidum through activation of caspase-3. Oncology Letters, 2, 565-570. https://doi.org/10.3892/ol.2011.275
MLA
Ji, Z., Tang, Q., Hao, R., Zhang, J., Pan, Y."Induction of apoptosis in the SW620 colon carcinoma cell line by triterpene-enriched extracts from Ganoderma lucidum through activation of caspase-3". Oncology Letters 2.3 (2011): 565-570.
Chicago
Ji, Z., Tang, Q., Hao, R., Zhang, J., Pan, Y."Induction of apoptosis in the SW620 colon carcinoma cell line by triterpene-enriched extracts from Ganoderma lucidum through activation of caspase-3". Oncology Letters 2, no. 3 (2011): 565-570. https://doi.org/10.3892/ol.2011.275
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