Establishment of primary mouse lung adenocarcinoma cell culture

Luo,Shuli; Sun,Mei; Jiang,Rui; Wang,Guan; Zhang,Xinyi

doi:10.3892/ol.2011.301

Establishment of primary mouse lung adenocarcinoma cell culture

Authors:
- Shuli Luo
- Mei Sun
- Rui Jiang
- Guan Wang
- Xinyi Zhang
View Affiliations

Affiliations: Department of Thoracic Surgery, The Second Hospital of Jilin University, Changchun 130041, P.R. China, Department of Pathology, The Second Hospital of Jilin University, Changchun 130041, P.R. China, Department of Orthopedic Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, P.R. China, Department of Cardiac Medicine, The Second Hospital of Jilin University, Changchun 130041, P.R. China
Published online on: May 9, 2011 https://doi.org/10.3892/ol.2011.301
Pages: 629-632

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Lung cancer is the most common malignant disease worldwide and is the leading cause of death from cancer. Primary cultures derived from lung cancer are essential for understanding abnormal growth function in lung epithelia. In this study, 2 out of 5 primary lung adenocarcinoma cultures derived from DNA repair-deficient mice were established and characterised using electron microscopy and immunostaining. Results of the tumourigenicity tests confirmed that these primary cells are tumourigenic. In conclusion, an effective primary culture method provides a tool for clinical antitumour drug testing.

View Figures

View References

1	Stewart BW and Kleihues P: World Cancer Report. IARC Press; Lyon: pp. 1822003
2	Butnor KJ and Beasley MB: Resolving dilemmas in lung cancer staging and histologic typing. Arch Pathol Lab Med. 131:1014–1015. 2007.PubMed/NCBI
3	Mulshine JL and Henschke CI: Prospects for lung-cancer screening. Lancet. 355:592–593. 2000. View Article : Google Scholar : PubMed/NCBI
4	Wright GS and Gruidl ME: Early detection and prevention of lung cancer. Curr Opin Oncol. 12:143–148. 2000. View Article : Google Scholar : PubMed/NCBI
5	Zhang X, Miao X, Liang G, et al: Polymorphisms in DNA base excision repair genes ADPRT and XRCC1 and risk of lung cancer. Cancer Res. 65:722–726. 2005.PubMed/NCBI
6	Shao M, Ma H, Wang Y, et al: Polymorphisms in excision repair cross-complementing group 4 (ERCC4) and susceptibility to primary lung cancer in a Chinese Han population. Lung Cancer. 60:332–339. 2008. View Article : Google Scholar : PubMed/NCBI
7	Bartek J, Lukas J and Bartkova J: DNA damage response as an anti-cancer barrier: damage threshold and the concept of ‘conditional haploinsufficiency’. Cell Cycle. 6:2344–2347. 2007.PubMed/NCBI
8	Tong WM, Cortes U, Hande MP, et al: Synergistic role of Ku80 and poly(ADP-ribose) polymerase in suppressing chromosomal aberrations and liver cancer formation. Cancer Res. 62:6990–6996. 2002.PubMed/NCBI
9	Tong WM, Yang YG, Cao WH, et al: Poly(ADP-ribose) polymerase-1 plays a role in suppressing mammary tumourigenesis in mice. Oncogene. 26:3857–3867. 2007. View Article : Google Scholar : PubMed/NCBI
10	Tong W-M, Hande MP, Lansdorp PM and Wang ZQ: DNA-strand break-sensing molecule PARP cooperates with p53 in telomere function, chromosomal stability and tumour suppression. Mol Cell Biol. 21:4046–4054. 2001. View Article : Google Scholar : PubMed/NCBI
11	Wang CG, Sun M, Zhao XJ and Zhang XY: Effect of STAT3 siRNA-induced inhibition of STAT3 gene expression on the growth and apoptosis of Lewis lung cancer cells. Chin J Clin Oncol. 3:392–399. 2006. View Article : Google Scholar
12	Wang CG, Wang RY, Sun M, et al: In vivo antitumour effect of siRNA against STAT3 on transplanted Lewis lung cancer in mice. Chem Res Chin Univ. 24:322–329. 2008.
13	Tong WM, Ohgaki H, Huang H, Granier C, Kleihues P and Wang ZQ: Null mutation of DNA strand break-binding molecule poly(ADP-ribose) polymerase causes medulloblastomas in p53(−/−) mice. Am J Pathol. 162:343–352. 2003.PubMed/NCBI
14	Yang S, Su J, Cao J, Zhang P, Lu J and Xie W: Establishment of a novel Chinese human lung adenocarcinoma cell line CPA-Yang1 which produces highly bone metastases in immunodeficient mice. Zhongguo Fei Ai Za Zhi. 12:753–759. 2009.PubMed/NCBI
15	Yaghi A, Zaman A and Dolovich M: Primary human bronchial epithelial cells grown from explants. J Vis Exp. 26:pii1789. View Article : Google Scholar
16	Kalinina T, Gungor C, Thieltges S, et al: Establishment and characterization of a new human pancreatic adenocarcinoma cell line with high metastatic potential to the lung. BMC Cancer. 10:2952010. View Article : Google Scholar : PubMed/NCBI
17	Higashiyama M, Oda K, Okami J, et al: Prediction of chemotherapeutic effect on postoperative recurrence by in vitro anticancer drug sensitivity testing in non-small cell lung cancer patients. Lung Cancer. 68:472–477. 2010. View Article : Google Scholar : PubMed/NCBI
18	Camps C, Sirera R, Iranzo V, Taron M and Rosell R: Gene expression and polymorphisms of DNA repair enzymes: cancer susceptibility and response to chemotherapy. Clin Lung Cancer. 8:369–375. 2007. View Article : Google Scholar : PubMed/NCBI
19	Hardwicke MA, Oleykowski CA, Plant R, et al: GSK1070916, a potent Aurora B/C kinase inhibitor with broad antitumour activity in tissue culture cells and human tumour xenograft models. Mol Cancer Ther. 8:1808–1817. 2009. View Article : Google Scholar
20	Powell C, Mikropoulos C, Kaye SB, et al: Pre-clinical and clinical evaluation of PARP inhibitors as tumour-specific radiosensitisers. Cancer Treat Rev. 36:566–575. 2010. View Article : Google Scholar : PubMed/NCBI