Inhibitory effect of vitamin D-binding protein‑derived macrophage activating factor on DMBA-induced hamster cheek pouch carcinogenesis and its derived carcinoma cell line

  • Authors:
    • Yukiyo Toyohara
    • Susumu Hashitani
    • Hiromitsu Kishimoto
    • Kazuma Noguchi
    • Nobuto Yamamoto
    • Masahiro Urade
  • View Affiliations

  • Published online on: May 13, 2011     https://doi.org/10.3892/ol.2011.306
  • Pages: 685-691
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

This study investigated the inhibitory effect of vitamin D-binding protein-derived macrophage-activating factor (GcMAF) on carcinogenesis and tumor growth, using a 9,10-dimethyl-1,2-benzanthracene (DMBA)-induced hamster cheek pouch carcinogenesis model, as well as the cytocidal effect of activated macrophages against HCPC-1, a cell line established from DMBA-induced cheek pouch carcinoma. DMBA application induced squamous cell carcinoma in all 15 hamsters of the control group at approximately 10 weeks, and all 15 hamsters died of tumor burden within 20 weeks. By contrast, 2 out of the 14 hamsters with GcMAF administration did not develop tumors and the remaining 12 hamsters showed a significant delay of tumor development for approximately 3.5 weeks. The growth of tumors formed was significantly suppressed and none of the hamsters died within the 20 weeks during which they were observed. When GcMAF administration was stopped at the 13th week of the experiment in 4 out of the 14 hamsters in the GcMAF-treated group, tumor growth was promoted, but none of the mice died within the 20-week period. On the other hand, when GcMAF administration was commenced after the 13th week in 5 out of the 15 hamsters in the control group, tumor growth was slightly suppressed and all 15 hamsters died of tumor burden. However, the mean survival time was significantly extended. GcMAF treatment activated peritoneal macrophages in vitro and in vivo, and these activated macrophages exhibited a marked cytocidal effect on HCPC-1 cells. Furthermore, the cytocidal effect of activated macrophages was enhanced by the addition of tumor-bearing hamster serum. These findings indicated that GcMAF possesses an inhibitory effect on tumor development and growth in a DMBA-induced hamster cheek pouch carcinogenesis model.
View Figures
View References

Related Articles

Journal Cover

July-August 2011
Volume 2 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Toyohara Y, Hashitani S, Kishimoto H, Noguchi K, Yamamoto N and Urade M: Inhibitory effect of vitamin D-binding protein‑derived macrophage activating factor on DMBA-induced hamster cheek pouch carcinogenesis and its derived carcinoma cell line. Oncol Lett 2: 685-691, 2011
APA
Toyohara, Y., Hashitani, S., Kishimoto, H., Noguchi, K., Yamamoto, N., & Urade, M. (2011). Inhibitory effect of vitamin D-binding protein‑derived macrophage activating factor on DMBA-induced hamster cheek pouch carcinogenesis and its derived carcinoma cell line. Oncology Letters, 2, 685-691. https://doi.org/10.3892/ol.2011.306
MLA
Toyohara, Y., Hashitani, S., Kishimoto, H., Noguchi, K., Yamamoto, N., Urade, M."Inhibitory effect of vitamin D-binding protein‑derived macrophage activating factor on DMBA-induced hamster cheek pouch carcinogenesis and its derived carcinoma cell line". Oncology Letters 2.4 (2011): 685-691.
Chicago
Toyohara, Y., Hashitani, S., Kishimoto, H., Noguchi, K., Yamamoto, N., Urade, M."Inhibitory effect of vitamin D-binding protein‑derived macrophage activating factor on DMBA-induced hamster cheek pouch carcinogenesis and its derived carcinoma cell line". Oncology Letters 2, no. 4 (2011): 685-691. https://doi.org/10.3892/ol.2011.306