Basic fibroblast growth factor induces cholangiocarcinoma cell migration via activation of the MEK1/2 pathway

  • Authors:
    • Siriluck Narong
    • Kawin Leelawat
  • View Affiliations

  • Published online on: June 29, 2011     https://doi.org/10.3892/ol.2011.333
  • Pages: 821-825
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The purpose of this study was to investigate the roles played by basic fibroblast growth factor (bFGF) in the induction of cholangiocarcinoma cell progression and to identify the signal transduction molecules that are activated by bFGF in cholangiocarcinoma cells. FGF receptor-2 (FGFR2) was shown to be expressed in two cholangiocarcinoma cell lines (RMCCA1 and KKU-100). Samples from RMCCA1 and KKU-100 were assayed for the mRNA. Phosphorylation levls were determined by Western blotting. Treatment of the cholangiocarcinoma cells with bFGF enhanced signaling via the phosphorylation of MEK1/2, induced cholangiocarcinoma cell migration and resulted in high levels of actin polymerization. Moreover, treatment with a MEK1/2 inhibitor (U0126) attenuated the effect of bFGF-induced cholangiocarcinoma cell migration. Taken together, these observations indicate that bFGF enhances the migration of cholangiocarcinoma cells and that this enhancement is regulated by the phosphorylation of MEK1/2.

Related Articles

Journal Cover

September-October 2011
Volume 2 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Narong S and Leelawat K: Basic fibroblast growth factor induces cholangiocarcinoma cell migration via activation of the MEK1/2 pathway. Oncol Lett 2: 821-825, 2011.
APA
Narong, S., & Leelawat, K. (2011). Basic fibroblast growth factor induces cholangiocarcinoma cell migration via activation of the MEK1/2 pathway. Oncology Letters, 2, 821-825. https://doi.org/10.3892/ol.2011.333
MLA
Narong, S., Leelawat, K."Basic fibroblast growth factor induces cholangiocarcinoma cell migration via activation of the MEK1/2 pathway". Oncology Letters 2.5 (2011): 821-825.
Chicago
Narong, S., Leelawat, K."Basic fibroblast growth factor induces cholangiocarcinoma cell migration via activation of the MEK1/2 pathway". Oncology Letters 2, no. 5 (2011): 821-825. https://doi.org/10.3892/ol.2011.333