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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Article

Expression of ERα36 in gastric cancer samples and their matched normal tissues

  • Authors:
    • Jianjun Wang
    • Jiajia Li
    • Rengui Fang
    • Shuduo Xie
    • Linbo Wang
    • Chaoyang Xu
  • View Affiliations / Copyright

    Affiliations: Department of Surgical Oncology, The Chunan County First Hospital, Zhejiang 312030, P.R. China, Department of Breast and Thyroid Surgery, Shaoxing People's Hospital, The First Affiliated Hospital of Shaoxing University, Zhejiang 312000, P.R. China, Department of Surgical Oncology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Zhejiang 310000, P.R. China, Department of Surgical Oncology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Zhejiang 310000, P.R. China
  • Pages: 172-175
    |
    Published online on: October 12, 2011
       https://doi.org/10.3892/ol.2011.437
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Abstract

Estrogen receptor α36 (ERα36) is believed to mediate membrane-initiated effects of estrogen signaling, and promote cell growth and resistance to tamoxifen treatment. However, few studies are available regarding ERα36 expression in gastric cancer. In the present study, we evaluated the expression of ERα36, as well as estrogen receptor α66 (ERα66), in gastric cancer and its correlation with clinicopathological parameters. Real-time polymerase chain reaction (PCR) was applied to detect the expression of ERα66 and ERα36 mRNA in 45 pairs of samples of gastric cancer tissues and matched normal tissues. The ΔΔCT method was used to evaluate the relative quantity of target mRNA expression. Among the 45 pairs of samples of gastric cancer tissues and matched normal tissues adjacent to the tumor, the ERα36 mRNA levels in normal tissues were significantly higher than those observed in gastric cancer tissues (p=0.040). Additionally, the expression of ERα66 mRNA levels between gastric cancer tissues and matched normal tissues had no statistically significant difference. We confirmed that ERα36 mRNA was expressed in the four gastric cancer cell lines, and ERα66 mRNA was expressed in two of the four gastric cancer cell lines. According to the tissue and cell findings, it was suggested that the expression level of ERα36 is greater than that of ERα66 in gastric cancer. In conclusion, the expression of ERα66 and ERα36 in gastric cancer tissues and cells was confirmed in this study. A decreased expression of ERα36 mRNA in gastric cancer tissues may be one of the factors affecting tumorigenesis in gastric cancer patients.
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Copy and paste a formatted citation
Spandidos Publications style
Wang J, Li J, Fang R, Xie S, Wang L and Xu C: Expression of ERα36 in gastric cancer samples and their matched normal tissues. Oncol Lett 3: 172-175, 2012.
APA
Wang, J., Li, J., Fang, R., Xie, S., Wang, L., & Xu, C. (2012). Expression of ERα36 in gastric cancer samples and their matched normal tissues. Oncology Letters, 3, 172-175. https://doi.org/10.3892/ol.2011.437
MLA
Wang, J., Li, J., Fang, R., Xie, S., Wang, L., Xu, C."Expression of ERα36 in gastric cancer samples and their matched normal tissues". Oncology Letters 3.1 (2012): 172-175.
Chicago
Wang, J., Li, J., Fang, R., Xie, S., Wang, L., Xu, C."Expression of ERα36 in gastric cancer samples and their matched normal tissues". Oncology Letters 3, no. 1 (2012): 172-175. https://doi.org/10.3892/ol.2011.437
Copy and paste a formatted citation
x
Spandidos Publications style
Wang J, Li J, Fang R, Xie S, Wang L and Xu C: Expression of ERα36 in gastric cancer samples and their matched normal tissues. Oncol Lett 3: 172-175, 2012.
APA
Wang, J., Li, J., Fang, R., Xie, S., Wang, L., & Xu, C. (2012). Expression of ERα36 in gastric cancer samples and their matched normal tissues. Oncology Letters, 3, 172-175. https://doi.org/10.3892/ol.2011.437
MLA
Wang, J., Li, J., Fang, R., Xie, S., Wang, L., Xu, C."Expression of ERα36 in gastric cancer samples and their matched normal tissues". Oncology Letters 3.1 (2012): 172-175.
Chicago
Wang, J., Li, J., Fang, R., Xie, S., Wang, L., Xu, C."Expression of ERα36 in gastric cancer samples and their matched normal tissues". Oncology Letters 3, no. 1 (2012): 172-175. https://doi.org/10.3892/ol.2011.437
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