Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
January 2012 Volume 3 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January 2012 Volume 3 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Isolation of hematopoietic stem cells and the effect of CD38 expression during the early erythroid progenitor cell development process

  • Authors:
    • Işıl Albeniz
    • Leyla Türker-Şener
    • Aycan Baş
    • İbrahim Kalelioğlu
    • Rüstem Nurten
  • View Affiliations / Copyright

    Affiliations: Istanbul University, Istanbul Faculty of Medicine, Department of Biophysics, Capa‑Istanbul 34093, Turkey, Department of Obstetrics and Gynecology, Istanbul University, Istanbul Faculty of Medicine, Capa-Istanbul 34093, Turkey
  • Pages: 55-60
    |
    Published online on: October 21, 2011
       https://doi.org/10.3892/ol.2011.455
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of this study was to investigate changes in primitive hematopoietic cells through CD38 expression, identify the stage at which erythrocyte differentiation CD38 gains activity and the effects of serum factors on this expression by establishing a hematopoietic stem cell system in the erythroid development process. Using an immunomagnetic labeling and separation technique, CD34+ cells were selected from cord blood. The CD34+ cells were cultured in a 2 mM L-glutamine‑enriched medium containing erythropoietin (Epo), penicillin‑streptomycin and stem cell factor (SCF), and were incubated in 5% CO2 at 37˚C. In erythroid development pathways following CD38 expression, primitive/progenitor human hematopoietic cells obtained from cord blood were assessed through the erythroid development process in a serum‑free medium in the presence of proper SCF and Epo. At the end of the 26‑day process, using staining with a Megacult-c staining kit, it was determined that progenitor cells nucleate and differentiate into erythroid cell lines of 8-10 µm. During the course of this process, we analyzed increases over time in NAD glycohydrolase activity rates using the supernatant liquid samples. Results of co-culture experiments in cell culture studies showed that the stimulating effects of CD38 expression originate from specific serum factors. CD38 expression has been shown to occur at hematopoietic cell sources as well as at a number of differentiation levels. In the proliferation process the possible induction of CD38 through specific serum factors leads us to conclude that it may be involved in proliferation with a physiological task or that it may be involved in an event, such as an apoptotic process.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Verfaillie CM, Pera MF and Lansdorp PM: Stem cells: hype and reality. Hematology. 1:369–391. 2002. View Article : Google Scholar

2 

Mitchell KE, Weiss ML, Mitchell BM and Martin P: Matrix cells from Wharton’s jelly from neurons and glia. Stem cells. 21:50–60. 2003.

3 

Czyz J, Wiese C, Rolletschek A, Blyszczuk P, Cross M and Wobus AM: Potential of embryonic and adult stem cells in vitro. Biol Chem. 384:1391–1409. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Malik P, Fisher TC, Barsky LW, Zeng L, Izadi P, Hiti AL, Weinberg KI, Coates TD, Meiselman HJ and Kohn DB: An in vitro model of human red blood cell production from hematopoietic progenitor cells. Blood. 91:2664–2671. 1998.PubMed/NCBI

5 

Bickenbach JR and Stem MM: Plasticity of epidermal stem cell: survival in various environments. Stem Cell Rev. 1:71–77. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Sato N, Sanjuan IM, Heke M, Naef F and Brivaniou AH: Molecular signature of human embryonic stem cells and its comporation with the mouse. Dev Biol. 260:404–413. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Audet J, Miller CL, Rose-John S, Piret JM and Eaves CJ: Distinct role of gp130 activation in promoting self-renewal divisions by mitogenically stimulated murine hematopoietic stem cells. Proc Natl Acad Sci USA. 98:1757–1762. 2001. View Article : Google Scholar : PubMed/NCBI

8 

Forsberg EC, Prohaska SS, Katzman S, Heffner GC, Stuart JM and Weissman IL: Differential expression of novel potential regulators in hematopoietic stem cells. PLoS Genet. 1:281–294. 2005. View Article : Google Scholar

9 

Venezia TA, Merchant AA, Ramos CA, Whitehouse NL, Young AS, Shaw CA and Goodell MA: Molecular signatures of proliferation and quiescence in hematopoietic stem cells. PLoS Biol. 2:1640–1651. 2004. View Article : Google Scholar : PubMed/NCBI

10 

Steidl U, Kronenwett R, Rohr UP, Fenk R, Kliszewski S, Maercker C, Neubert P, Aivado M, Koch J, Modlich O, et al: Gene expression profiling identifies significant differences between the molecular phenotypes of bone marrow-derived and circulating human CD34+ hematopoietic stem cells. Blood. 99:2037–44. 2002.PubMed/NCBI

11 

Wognum WA, Eaves CA and Thomas ET: Identification and isolation of hematopoietic stem cells. Arch Med Res. 34:461–475. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Albeniz I, Demir Ö, Türker-Şener L, Yalçıntepe L, Nurten R and Bermek E: Erythrocyte CD38 as a prognostic marker in cancer. Hematology. 12:409–414. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Funaro A, Reinis M, Trubiani O, Santi S, Di Primio R and Malavasi F: CD38 functions are regulated through an internalization step. J Immunol. 160:2238–2247. 1998.PubMed/NCBI

14 

Mainou-Fowler T, Dignum HM, Proctor SJ and Summerfield GP: The prognostic value of CD38 expression and its quantification in B cell chronic lymphocytic leukemia (B-CLL). Leuk Lymphoma. 45:455–462. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Matrai Z: CD38 as a prognostic marker in CLL. Hematology. 10:39–46. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Akel S, Petrow-Sadowski C, Laughlin MJ and Ruscett FW: Neutralization of autocrine transforming growth factor-β in human cord blood CD34+CD38−Lin− cell promotes stem cell factor-mediated erythropoetin-independent early erythroid progenitor development and reduces terminal differentiation. Stem Cells. 21:557–567. 2003.

17 

Kim H, Jacobson EL and Jacobson MK: Synthesis and degradation of cyclic ADP-ribose by NAD glycohydrolase. Science. 261:1330–1333. 1993. View Article : Google Scholar : PubMed/NCBI

18 

Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:680–685. 1970. View Article : Google Scholar : PubMed/NCBI

19 

Gershoni JM and Palade GE: Protein blotting: Principles and applications. Anal Biochem. 131:1–15. 1983. View Article : Google Scholar : PubMed/NCBI

20 

Storms RW, Green PD, Safford KM, Niedzwiecki D, Cogle CR, Colvin OM, Chao NJ, Rice HE and Smith CA: Distinct hematopoietic progenitor compartments are delineated by the expression of aldehyde dehydrogenase and CD34. Blood. 106:95–102. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Hao QL, Smogorzewska EM, Barsky LW and Crooks GM: In vitro identification of single CD34+CD38− cells with both lymphoid and myeloid potential. Blood. 91:4145–4151. 1998.

22 

Neildez-Nguyen TM, Wajcman H, Marden MC, Bensidhoum M, Moncollin V, Giarratana MC, Kobari L, Thierry D and Douay L: Human erythroid cells produced ex vivo at large scale differentiate into red blood cells in vivo. Nature Biotechnology. 20:467–472. 2002. View Article : Google Scholar : PubMed/NCBI

23 

Dorn I, Lazar-Karsten P, Boie S, Ribbat J, Hartwig D, Driller B, Kirchner H and Schlenke P: In vitro proliferation and differentiation of human CD34+ cells from peripheral blood into mature red blood cells with two different cell culture systems. Transplant Cell Eng. 48:1122–1132. 2008.PubMed/NCBI

24 

Jacobson MK, Laurean DC, Strohm MS and Jacobson EL: NAD glycohydrolase and the metabolism of cyclic ADP-ribose. Biochimie. 77:341–344. 1995. View Article : Google Scholar : PubMed/NCBI

25 

Zocchi E, Franco L, Guida L, Benatti U, Bargellesi A, Malavasi F, Lee HC and De Flora A: A single protein immunologically identified as CD38 displays NAD+ glycohydrolase, ADP-ribosylcyclase and ADP-ribose hydrolase activities at the outer surface of erythrocytes. Biochem Biophys Res Commun. 196:1459–1465. 1993.PubMed/NCBI

26 

Albeniz I, Demir Ö, Nurten R and Bermek E: NAD glycohydrolase activities and ADP-ribose uptake in erythrocytes from normal subject and cancer patients. Bioscience Rep. 24:41–53. 2004. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Albeniz I, Türker-Şener L, Baş A, Kalelioğlu İ and Nurten R: Isolation of hematopoietic stem cells and the effect of CD38 expression during the early erythroid progenitor cell development process. Oncol Lett 3: 55-60, 2012.
APA
Albeniz, I., Türker-Şener, L., Baş, A., Kalelioğlu, İ., & Nurten, R. (2012). Isolation of hematopoietic stem cells and the effect of CD38 expression during the early erythroid progenitor cell development process. Oncology Letters, 3, 55-60. https://doi.org/10.3892/ol.2011.455
MLA
Albeniz, I., Türker-Şener, L., Baş, A., Kalelioğlu, İ., Nurten, R."Isolation of hematopoietic stem cells and the effect of CD38 expression during the early erythroid progenitor cell development process". Oncology Letters 3.1 (2012): 55-60.
Chicago
Albeniz, I., Türker-Şener, L., Baş, A., Kalelioğlu, İ., Nurten, R."Isolation of hematopoietic stem cells and the effect of CD38 expression during the early erythroid progenitor cell development process". Oncology Letters 3, no. 1 (2012): 55-60. https://doi.org/10.3892/ol.2011.455
Copy and paste a formatted citation
x
Spandidos Publications style
Albeniz I, Türker-Şener L, Baş A, Kalelioğlu İ and Nurten R: Isolation of hematopoietic stem cells and the effect of CD38 expression during the early erythroid progenitor cell development process. Oncol Lett 3: 55-60, 2012.
APA
Albeniz, I., Türker-Şener, L., Baş, A., Kalelioğlu, İ., & Nurten, R. (2012). Isolation of hematopoietic stem cells and the effect of CD38 expression during the early erythroid progenitor cell development process. Oncology Letters, 3, 55-60. https://doi.org/10.3892/ol.2011.455
MLA
Albeniz, I., Türker-Şener, L., Baş, A., Kalelioğlu, İ., Nurten, R."Isolation of hematopoietic stem cells and the effect of CD38 expression during the early erythroid progenitor cell development process". Oncology Letters 3.1 (2012): 55-60.
Chicago
Albeniz, I., Türker-Şener, L., Baş, A., Kalelioğlu, İ., Nurten, R."Isolation of hematopoietic stem cells and the effect of CD38 expression during the early erythroid progenitor cell development process". Oncology Letters 3, no. 1 (2012): 55-60. https://doi.org/10.3892/ol.2011.455
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team