Single nucleotide polymorphisms in DNA repair genes and risk of cervical cancer: A case-control study

Zhang,Lihua; Ruan,Zhenchao; Hong,Qingya; Gong,Xiangzhen; Hu,Zhengguang; Huang,Yan; Xu,Aidi

doi:10.3892/ol.2011.463

Single nucleotide polymorphisms in DNA repair genes and risk of cervical cancer: A case-control study

Authors:
- Lihua Zhang
- Zhenchao Ruan
- Qingya Hong
- Xiangzhen Gong
- Zhengguang Hu
- Yan Huang
- Aidi Xu
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Affiliations: Department of Radiation Oncology, Cancer Hospital, Fudan University, Shanghai 200032, P.R. China, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, P.R. China, Hospital of Obstetrics and Gynecology, Attached Fudan University, Shanghai 200011, P.R. China, Hongkou Maternal and Child Health Care Institute, Shanghai 200437, P.R. China, Shanghai Hongkou District Health Bureau, Shanghai 200086, P.R. China
Published online on: October 26, 2011 https://doi.org/10.3892/ol.2011.463
Pages: 351-362

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Abstract

In this report, we describe a case control study in a Chinese population aimed at identifying possible associations between susceptibility to cervical cancer and single nucleotide polymorphisms in XRCC1 194C>T, XRCC1 280G>A, XRCC1 399G>A, ERCC2 751A>C , ERCC2 156C>A, ERCC1 118C>T, PARP1 762T>C , RAD51 135G>C and HER2 655A>G. The cases comprised 154 patients: 80 cervical squamous cell carcinomas (SCCs), 2 adenocarcinomas and 72 cervical intraepithelial neoplasias (CINs). A total of 177 healthy women were recruited as the controls. A significant association was found between ERCC1 118C>T and SCC in the additive genetic model [odds ratio (OR)=1.711; 95% confidence interval (CI), 1.089-2.880; p=0.021] and the dominant genetic model (OR=1.947; 95% CI, 1.056-3.590; p=0.033). Among women with a smoking family member, ERCC1 118C>T increased SCC risk in the additive model (OR=2.800; 95% CI, 1.314‑5.968; p=0.008). For women who had first intercourse before 22 years of age, XRCC1 280G>A was found to act as a protective factor for SCC under the additive model (OR=0.228; 95% CI, 0.058‑0.900; p=0.035), while RAD51 135G>C was a risk factor for CIN (OR=4.246; 95% CI, 1.335-13.502; p=0.014). For women who had first intercourse after 22 years of age, the additive genetic model showed RAD51 135G>C (OR=0.359; 95% CI, 0.138-0.934; p=0.036) and HER2 655A>G (OR=0.309; 95% CI, 0.098-0.972; p=0.045) to be protective factors for SCC. XRCC1 399G>A increased CIN risk among women who first gave birth before the age of 22 in the additive genetic model (OR=4.459; 95% CI, 1.139‑17.453; p=0.032). For those who first gave birth after age 22, ERCC1 118C>T was found to be a risk factor for SCC in the additive genetic model (OR=1.884; 95% CI, 1.088-3.264; p=0.024). A significant interaction was observed between RAD51 135G>C and age at first intercourse (pinteraction=0.033 for SCC, pinteraction=0.021 for CIN), as well with sexual partner number (pinteraction=0.001 for SCC). The interaction between HER2 655A>G and age at first intercourse, ERCC2 156C>A and family smoking status and XRCC1 280G>A and alcohol consumption were significant, with pinteraction=0.023 for SCC, pinteraction=0.021 for CIN and pinteraction=0.025 for SCC, respectively.

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