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Article

C-Src-mediated RANKL-induced breast cancer cell migration by activation of the ERK and Akt pathway

  • Authors:
    • Lingyun Zhang
    • Yuee Teng
    • Ye Zhang
    • Jing Liu
    • Ling Xu
    • Jinglei  Qu
    • Kezuo Hou
    • Xianghong Yang
    • Yunpeng Liu
    • Xiujuan Qu
  • View Affiliations / Copyright

    Affiliations: Department of Medical Oncology, the First Hospital of China Medical University, Shenyang 110001, P.R. China, Department of Pathology, Shengjing Hospital of China Medical University, Shenyang 110001, P.R. China
  • Pages: 395-400
    |
    Published online on: November 16, 2011
       https://doi.org/10.3892/ol.2011.487
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Abstract

The receptor activator for nuclear factor κB ligand/receptor activator for nuclear factor κB (RANKL/RANK) pathway is critical for RANK-expressing cancer cells to home to bones, and c-Src is critical for cancer progression. The objective of this study was to explore the effect of c-Src in the RANKL/RANK pathway and migration activity in human breast cancer cells. Breast cancer cell lines MCF-7, MDA-MB‑231 and BT-474 were obtained and cultured. Flow cytometry was used to examine RANK expression. The results showed that RANK was expressed in breast cancer cell lines MCF-7, MDA-MB‑231 and BT-474, and soluble RANKL (sRANKL)-triggered migration of breast cancer cells by activating ERK1/2, Akt and c-Src. The sRANKL-induced migration was blocked with RANKL inhibitor osteoprotegerin (OPG), MEK inhibitor PD98059, PI3K inhibitor LY294002 and Src inhibitor PP2. Inhibition of c-Src function with PP2 blocked the activation of Akt and ERK1/2, resulting in the inhibition of RANKL-induced migration. In conclusion, RANKL was found to increase the migration of breast cancer cells by activating the c-Src-Akt and c-Src-ERK signaling pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang L, Teng Y, Zhang Y, Liu J, Xu L, Qu J, Hou K, Yang X, Liu Y, Qu X, Qu X, et al: C-Src-mediated RANKL-induced breast cancer cell migration by activation of the ERK and Akt pathway. Oncol Lett 3: 395-400, 2012.
APA
Zhang, L., Teng, Y., Zhang, Y., Liu, J., Xu, L., Qu, J. ... Qu, X. (2012). C-Src-mediated RANKL-induced breast cancer cell migration by activation of the ERK and Akt pathway. Oncology Letters, 3, 395-400. https://doi.org/10.3892/ol.2011.487
MLA
Zhang, L., Teng, Y., Zhang, Y., Liu, J., Xu, L., Qu, J., Hou, K., Yang, X., Liu, Y., Qu, X."C-Src-mediated RANKL-induced breast cancer cell migration by activation of the ERK and Akt pathway". Oncology Letters 3.2 (2012): 395-400.
Chicago
Zhang, L., Teng, Y., Zhang, Y., Liu, J., Xu, L., Qu, J., Hou, K., Yang, X., Liu, Y., Qu, X."C-Src-mediated RANKL-induced breast cancer cell migration by activation of the ERK and Akt pathway". Oncology Letters 3, no. 2 (2012): 395-400. https://doi.org/10.3892/ol.2011.487
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang L, Teng Y, Zhang Y, Liu J, Xu L, Qu J, Hou K, Yang X, Liu Y, Qu X, Qu X, et al: C-Src-mediated RANKL-induced breast cancer cell migration by activation of the ERK and Akt pathway. Oncol Lett 3: 395-400, 2012.
APA
Zhang, L., Teng, Y., Zhang, Y., Liu, J., Xu, L., Qu, J. ... Qu, X. (2012). C-Src-mediated RANKL-induced breast cancer cell migration by activation of the ERK and Akt pathway. Oncology Letters, 3, 395-400. https://doi.org/10.3892/ol.2011.487
MLA
Zhang, L., Teng, Y., Zhang, Y., Liu, J., Xu, L., Qu, J., Hou, K., Yang, X., Liu, Y., Qu, X."C-Src-mediated RANKL-induced breast cancer cell migration by activation of the ERK and Akt pathway". Oncology Letters 3.2 (2012): 395-400.
Chicago
Zhang, L., Teng, Y., Zhang, Y., Liu, J., Xu, L., Qu, J., Hou, K., Yang, X., Liu, Y., Qu, X."C-Src-mediated RANKL-induced breast cancer cell migration by activation of the ERK and Akt pathway". Oncology Letters 3, no. 2 (2012): 395-400. https://doi.org/10.3892/ol.2011.487
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